Probe-Based Fluorescence Melting Curve Analysis for Differentiating Larimichthys polyactis and Larimichthys crocea

2016 ◽  
Vol 9 (7) ◽  
pp. 2036-2041 ◽  
Author(s):  
Jeong Jin Ahn ◽  
Youngjoo Kim ◽  
Ji Young Hong ◽  
Gi Won Kim ◽  
Seol Young Kim ◽  
...  
Keyword(s):  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Larimichthys Crocea ◽  
Larimichthys Polyactis ◽  
Fluorescence Melting Curve Analysis

scholarly journals Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Liuyang Hu ◽  
Bing Han ◽  
Qin Tong ◽  
Hui xiao ◽  
Donglin Cao
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Bacterial Pathogens ◽  
Melting Curve ◽  
Cost Effective ◽  
Culture Method ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Fluorescence Melting Curve Analysis

Background and Objective. Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Pseudomonas aeruginosa, and Mycobacterium tuberculosis are primary respiratory bacterial pathogens contributing to morbidity and mortality in developing countries. This study evaluated the diagnostic performance of multiplex real-time PCR with fluorescence melting curve analysis (MCA) assay, which was used to detect eight respiratory bacterial pathogens simultaneously. Methods. A total of 157 sputum specimens were examined by multiplex real-time with fluorescence MCA, and the results were compared with the conventional culture method. Results. Multiplex real-time PCR with fluorescence MCA specifically detected and differentiated eight respiratory bacterial pathogens by different melting curve peaks for each amplification product within 2 hours and exhibited high repeatability. The limit of detection ranged from 64 to 102 CFU/mL in the multiplex PCR system. Multiplex real-time PCR with fluorescence MCA showed a sensitivity greater than 80% and a 100% specificity for each pathogen. The kappa correlation of eight bacteria ranged from 0.89 to 1.00, and the coefficient of variation ranged from 0.05% to 0.80%. Conclusions. Multiplex real-time PCR with fluorescence MCA assay is a sensitive, specific, high-throughput, and cost-effective method to detect multiple bacterial pathogens simultaneously.

A new method for ABO genotyping using fluorescence melting curve analysis based on peptide nucleic acid probes

2015 ◽  
Vol 17 (5) ◽  
pp. 334-339 ◽  
Author(s):  
Kyungmyung Lee ◽  
Hyun-Chul Park ◽  
Sanghyun An ◽  
Eu-Ree Ahn ◽  
Yang-Han Lee ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Melting Curve ◽  
Curve Analysis ◽  
New Method ◽  
Melting Curve Analysis ◽  
Nucleic Acid Probes ◽  
Fluorescence Melting Curve Analysis

Detection of vaccinia virus DNA on the LightCycler by fluorescence melting curve analysis

2005 ◽  
Vol 126 (1-2) ◽  
pp. 187-195 ◽  
Author(s):  
Andreas Nitsche ◽  
Brigitte Steger ◽  
Heinz Ellerbrok ◽  
Georg Pauli
Keyword(s):  
Vaccinia Virus ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Fluorescence Melting Curve Analysis

Rapid Differentiation of Seven Species of Anguilla Using PNA Clamping-based Asymmetric PCR with Fluorescence Melting Curve Analysis

2018 ◽  
Vol 12 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Eun Soo Noh ◽  
Hyun Sook Kang ◽  
Eun Mi Kim ◽  
Jae Koo Noh ◽  
Jung Youn Park ◽  
...  
Keyword(s):  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Asymmetric Pcr ◽  
Fluorescence Melting Curve Analysis

scholarly journals Detection of IDH1 and IDH2 Mutations by Fluorescence Melting Curve Analysis as a Diagnostic Tool for Brain Biopsies

2010 ◽  
Vol 12 (4) ◽  
pp. 487-492 ◽  
Author(s):  
Craig Horbinski ◽  
Lindsey Kelly ◽  
Yuri E. Nikiforov ◽  
Mary Beth Durso ◽  
Marina N. Nikiforova
Keyword(s):  
Diagnostic Tool ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Fluorescence Melting Curve Analysis ◽  
Idh1 And Idh2 Mutations
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