Seed Coating with Mycorrhizal Fungal Spores and LEIFSONIA Bacteria: A Tool for Microbiological Fertilization and a Seed Protection Strategy From Insect Damage

Background: User behavior models have been widely used to simulate attack behaviors in the security domain. We revised all patents related to response to attack behavior models. How to decide the protected target against multiple models of attack behaviors is studied. Methods: We utilize one perfect rational and three bounded rational behavior models to simulate attack behaviors in cloud computing, and then investigate cloud provider’s response based on Stackelberg game. The cloud provider plays the role of defender and it is assumed to be intelligent enough to predict the attack behavior model. Based on the prediction accuracy, two schemes are built in two situations. Results: If the defender can predict the attack behavior model accurately, a single-objective game model is built to find the optimal protection strategy; otherwise, a multi-objective game model is built to find the optimal protection strategy. Conclusion: The numerical results prove that the game theoretic model performs better in the corresponding situation.

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Development and Application of Seed Coating Agent for the Control of Major Soil-Borne Diseases Infecting Wheat

Agronomy ◽

10.3390/agronomy9080413 ◽

2019 ◽

Vol 9 (8) ◽

pp. 413 ◽

Cited By ~ 3

Author(s):

Ren ◽

Chen ◽

Ye ◽

Su ◽

Xiao ◽

...

Keyword(s):

Inhibitory Effect ◽

Seed Coating ◽

Practical Application ◽

Active Constituent ◽

Coating Agent ◽

Grinding Method ◽

Fungal Inhibition ◽

Sharp Eyespot ◽

Coating Agents ◽

Seed Germination Test

In order to reduce the usage amount of pesticide fertilizers and protect the natural environment, seed coating agents are receiving increased wide concern. In this study, the active constituent (pesticide) and inactive components (surfactants and film former) of the seed coating agents were screened and optimized by the wet sand processing superfine grinding method. The fungal inhibition test of pesticides showed that thifluzamide, fludioxonil, pyraclostrobin, and difenoconazole have an obvious fungal inhibitory effect on wheat sharp eyespot, take-all, and root rot. LAE-9 and polyacrylamide + carboxymethyl cellulose (CMC) is recommended for the safe surfactant and film former, respectively, based on the seed germination test. Moreover, 6% difenoconazole · fludioxonil flowable concentrate for seed coating (FSC) stimulates the seedling growth of wheat, advances the growth of root, and improves biomass in the field trial, meanwhile, the control efficiency reached above 80%. Thus, we suggested it can be used as an effective seed coating agent for the control of soil-borne diseases in wheat. The seed coating agent has the characteristics of disease prevention, increasing crop yield, and safety of environment, which is of significance in practical application.

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Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

10.5194/acp-2018-1073 ◽

2018 ◽

Author(s):

Arnaldo Negron ◽

Natasha DeLeon-Rodriguez ◽

Samantha M. Waters ◽

Luke D. Ziemba ◽

Bruce Anderson ◽

...

Keyword(s):

Flow Cytometry ◽

Nucleic Acid ◽

Acid Content ◽

Fungal Spores ◽

Epifluorescence Microscopy ◽

Nucleic Acid Content ◽

Rain Event ◽

Bacterial Cells ◽

Sampling System ◽

Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

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