In vitro selection of drug resistant Schistosoma mansoni

1987 ◽  
Vol 17 (3) ◽  
pp. 767-771 ◽  
Author(s):  
G.C. Coles ◽  
J.I. Bruce
Keyword(s):  
Schistosoma Mansoni ◽  
In Vitro Selection ◽  
Drug Resistant ◽  
Selection Of

scholarly journals In vitro selection of Remdesivir resistance suggests evolutionary predictability of SARS-CoV-2

2021 ◽  
Vol 17 (9) ◽  
pp. e1009929
Author(s):  
Agnieszka M. Szemiel ◽  
Andres Merits ◽  
Richard J. Orton ◽  
Oscar A. MacLean ◽  
Rute Maria Pinto ◽  
...  
Keyword(s):  
Nucleoside Analogue ◽  
Reverse Genetics ◽  
High Throughput Sequencing ◽  
In Vitro Selection ◽  
Single Mutation ◽  
Drug Resistant ◽  
Immune Selection ◽  
Virus Surveillance ◽  
Selection Of

Remdesivir (RDV), a broadly acting nucleoside analogue, is the only FDA approved small molecule antiviral for the treatment of COVID-19 patients. To date, there are no reports identifying SARS-CoV-2 RDV resistance in patients, animal models or in vitro. Here, we selected drug-resistant viral populations by serially passaging SARS-CoV-2 in vitro in the presence of RDV. Using high throughput sequencing, we identified a single mutation in RNA-dependent RNA polymerase (NSP12) at a residue conserved among all coronaviruses in two independently evolved populations displaying decreased RDV sensitivity. Introduction of the NSP12 E802D mutation into our SARS-CoV-2 reverse genetics backbone confirmed its role in decreasing RDV sensitivity in vitro. Substitution of E802 did not affect viral replication or activity of an alternate nucleoside analogue (EIDD2801) but did affect virus fitness in a competition assay. Analysis of the globally circulating SARS-CoV-2 variants (>800,000 sequences) showed no evidence of widespread transmission of RDV-resistant mutants. Surprisingly, we observed an excess of substitutions in spike at corresponding sites identified in the emerging SARS-CoV-2 variants of concern (i.e., H69, E484, N501, H655) indicating that they can arise in vitro in the absence of immune selection. The identification and characterisation of a drug resistant signature within the SARS-CoV-2 genome has implications for clinical management and virus surveillance.

In vitro selection of DNA aptamers recognizing drug-resistant ovarian cancer by cell-SELEX

Talanta ◽  
2019 ◽  
Vol 194 ◽  
pp. 437-445 ◽  
Author(s):  
Junqing He ◽  
Junyan Wang ◽  
Nan Zhang ◽  
Luyao Shen ◽  
Linlin Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
In Vitro Selection ◽  
Dna Aptamers ◽  
Drug Resistant ◽  
Selection Of

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups

2000 ◽  
Vol 15 (4) ◽  
pp. 297-308 ◽  
Author(s):  
NAOZUMI TERAMOTO ◽  
YUKIO IMANISHI ◽  
YOSHIHIRO ITO
Keyword(s):  
In Vitro Selection ◽  
Amino Groups ◽  
Selection Of

scholarly journals Back Cover: In Vitro Selection of a DNA Aptamer Targeting Degraded Protein Fragments for Biosensing (Angew. Chem. Int. Ed. 20/2020)

2020 ◽  
Vol 59 (20) ◽  
pp. 7968-7968
Author(s):  
Meng Liu ◽  
Jiayi Wang ◽  
Yangyang Chang ◽  
Qiang Zhang ◽  
Dingran Chang ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Dna Aptamer ◽  
Protein Fragments ◽  
Back Cover ◽  
Selection Of
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