Effects of monoclonal antibody against phospholamban on calcium pump ATPase of cardiac sarcoplasmic reticulum

1991 ◽  
Vol 23 (11) ◽  
pp. 1223-1230 ◽  
Author(s):  
Y Kimura
Keyword(s):  
Monoclonal Antibody ◽  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Cardiac Sarcoplasmic Reticulum

Comparison of the Kinetic Effects of Phospholamban Phosphorylation and Anti-phospholamban Monoclonal Antibody on the Calcium Pump in Purified Cardiac Sarcoplasmic Reticulum Membranes†

Biochemistry ◽  
1997 ◽  
Vol 36 (42) ◽  
pp. 12903-12910 ◽  
Author(s):  
Alexander Y. Antipenko ◽  
Andrew I. Spielman ◽  
Massimo Sassaroli ◽  
Madeleine A. Kirchberger
Keyword(s):  
Monoclonal Antibody ◽  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Kinetic Effects ◽  
Phospholamban Phosphorylation

scholarly journals Phospholamban regulation of cardiac sarcoplasmic reticulum (Ca(2+)-Mg2+)-ATPase. Mechanism of regulation and site of monoclonal antibody interaction

1991 ◽  
Vol 266 (17) ◽  
pp. 11270-11275
Author(s):  
G.L. Morris ◽  
H.C. Cheng ◽  
J. Colyer ◽  
J.H. Wang
Keyword(s):  
Monoclonal Antibody ◽  
Sarcoplasmic Reticulum ◽  
Cardiac Sarcoplasmic Reticulum

Inhibitory and stimulatory effects of fluoride on the calcium pump of cardiac sarcoplasmic reticulum

1991 ◽  
Vol 1070 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Njanoor Narayanan ◽  
Na Su ◽  
Philip Bedard
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Cardiac Sarcoplasmic Reticulum

The reversal of the calcium pump of cardiac sarcoplasmic reticulum

1977 ◽  
Vol 72 (2-3) ◽  
pp. 147-152 ◽  
Author(s):  
J. Suko ◽  
G. Hellmann ◽  
F. Winkler
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Cardiac Sarcoplasmic Reticulum

Phospholamban, activator of the cardiac sarcoplasmic reticulum calcium pump. Physicochemical properties and diagonal purification

Biochemistry ◽  
1980 ◽  
Vol 19 (14) ◽  
pp. 3368-3373 ◽  
Author(s):  
Christian J. Le Peuch ◽  
Danielle A. M. Le Peuch ◽  
Jacques G. Demaille
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Physicochemical Properties ◽  
Calcium Pump ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Sarcoplasmic Reticulum Calcium

Inhibition of β-adrenergic stimulated calcium pump of rat cardiac sarcoplasmic reticulum by tricyclohexyltin hydroxide

1987 ◽  
Vol 5 (2) ◽  
pp. 149-154 ◽  
Author(s):  
I. Kabeer Ahammed Sahib ◽  
D. Desaiah
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Calcium Pump ◽  
Cardiac Sarcoplasmic Reticulum

scholarly journals Dwarf open reading frame (DWORF) peptide is a direct activator of the sarcoplasmic reticulum calcium pump SERCA

2020 ◽  
Author(s):  
M’Lynn E. Fisher ◽  
Elisa Bovo ◽  
Ellen E. Cho ◽  
Marsha P. Pribadi ◽  
Michael P. Dalton ◽  
...  
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Cardiac Muscle ◽  
Cytosolic Calcium ◽  
Open Reading Frame ◽  
Calcium Pump ◽  
Calcium Handling ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Membrane Reconstitution ◽  
Reading Frame ◽  
Sarcoplasmic Reticulum Calcium

ABSTRACTThe cardiac sarcoplasmic reticulum calcium pump, SERCA, sequesters calcium in the sarco-endoplasmic reticulum (SR/ER) and plays a critical role in the contraction-relaxation cycle of the heart. A well-known regulator of SERCA in cardiac muscle is phospholamban (PLN), which interacts with the pump and reduces its apparent calcium affinity. A newly discovered SERCA regulatory subunit in cardiac muscle, dwarf open reading frame (DWORF), has added a new level of SERCA regulation. In this report, we modeled the structure of DWORF and evaluated it using molecular dynamics simulations. DWORF structure was modeled as a discontinuous helix with an unwound region at Pro15. This model orients an N-terminal amphipathic helix along the membrane surface and leaves a relatively short C-terminal transmembrane helix. We determined the functional regulation of SERCA by DWORF using a membrane reconstitution system. Surprisingly, we observed that DWORF directly activated SERCA by increasing its turnover rate. Furthermore, in-cell imaging of calcium dynamics demonstrated that DWORF increased SERCA-dependent ER calcium load, calcium reuptake rate, and spontaneous calcium release. Together, these functional assays suggest opposing effects of DWORF and PLN on SERCA function. The results agree with fluorescence resonance energy transfer experiments, which revealed changes in the affinity of DWORF for SERCA at low versus high cytosolic calcium concentrations. We found that DWORF has a higher affinity for SERCA in the presence of calcium, while PLN had the opposite behavior, a higher affinity for SERCA in low calcium. We propose a new mechanism for DWORF regulation of cardiac calcium handling in which DWORF directly enhances SERCA turnover by stabilizing the conformations of SERCA that predominate during elevated cytosolic calcium.

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