Does glucagon improve the viability of ischemic intestine?

1990 ◽  
Vol 49 (6) ◽  
pp. 524-533 ◽  
Author(s):  
Atsushi Oshima ◽  
Masaki Kitajima ◽  
Noritaka Sakai ◽  
Nobutoshi Ando
Keyword(s):  
Ischemic Intestine

Conversion of xanthine dehydrogenase to oxidase in ischemic rat intestine: a reevaluation

1988 ◽  
Vol 254 (5) ◽  
pp. G768-G774 ◽  
Author(s):  
D. A. Parks ◽  
T. K. Williams ◽  
J. S. Beckman
Keyword(s):  
Small Intestine ◽  
Oxygen Radicals ◽  
Total Activity ◽  
Xanthine Dehydrogenase ◽  
Oxygen Radical ◽  
Morphological Evidence ◽  
Cellular Injury ◽  
Rat Intestine ◽  
Entire Small Intestine ◽  
Ischemic Intestine

Oxygen radicals derived from xanthine oxidase (XO) are important mediators of the cellular injury associated with reperfusion of ischemic intestine, stomach, liver, kidney, and pancreas. XO exists in nonischemic tissue predominantly as xanthine dehydrogenase (XDH) and converts to oxygen radical-producing XO with ischemia. Grinding intestine under liquid nitrogen and placing the powder in phosphate buffer (pH 7.0) containing thiol reductants and protease inhibitors adequately preserved total XDH + XO activity and the percentage in the oxidase form (%XO) for 24 h. Total activity in nonischemic intestine ranged from 374 nmol.min-1.g-1 in duodenum to 138 nmol.min-1.g-1 in ileum, while XO activity was approximately 19% of total activity throughout the entire small intestine. The rate of XDH conversion to XO during normothermic ischemia varied only slightly throughout the intestine, increasing 13% per hour to 34, 46, and 61% XO after 1, 2, and 3 h of ischemia, respectively. Our results contrast with previous reports where XDH conversion to XO occurred within 60 s ischemia but are consistent with physiological and morphological evidence of ischemic injury and provide further support for involvement of XO in intestinal injury associated with ischemia.

scholarly journals EXPERIMENTAL STUDIES ON THE EVALUATION OF THE VIABILITY OF THE ISCHEMIC INTESTINE BY THE FLUOROMETER

1983 ◽  
Vol 16 (4) ◽  
pp. 744-744
Author(s):  
Katsumi SASAKI ◽  
Hiroshi SHIGEMATSZ ◽  
Sigenobu OHASHI ◽  
Yasuhiko MORIOKA
Keyword(s):  
Experimental Studies ◽  
Ischemic Intestine

Biomagnetic Detection of Injury Currents in Rabbit Ischemic Intestine

2005 ◽  
Vol 50 (9) ◽  
pp. 1561-1568 ◽  
Author(s):  
L. Alan Bradshaw ◽  
Ornob P. Roy ◽  
Gavin P. O’Mahony ◽  
Andrew G. Myers ◽  
James G. McDowell ◽  
...  
Keyword(s):  
Ischemic Intestine

Evaluation of ischemic intestine by Doppler ultrasound

1980 ◽  
Vol 139 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Marc Cooperman ◽  
Edward W. Martin ◽  
Larry C. Carey
Keyword(s):  
Doppler Ultrasound ◽  
Ischemic Intestine

Adenine Nucleotides of Ischemic Intestine Do Not Reflect Injury

1993 ◽  
Vol 55 (4) ◽  
pp. 416-421 ◽  
Author(s):  
A.T. Canada ◽  
L.R. Coleman ◽  
M.A. Fabian ◽  
R.R. Bollinger
Keyword(s):  
Adenine Nucleotides ◽  
Ischemic Intestine

Sausage resection of ischemic intestine

1987 ◽  
Vol 22 (11) ◽  
pp. 1011-1012 ◽  
Author(s):  
Peter A. Schneider ◽  
Michael R. Harrison
Keyword(s):  
Ischemic Intestine

scholarly journals Pathomorphological manifestations of ischemic involvement of the small intestine in acute occlusion of the mesenteric artery

2018 ◽  
Vol 11 (4) ◽  
pp. 291-296
Author(s):  
Maksim Georgievich Ryabkov ◽  
Michael Sergeevich Baleev ◽  
Eugeniy Lvivna Bederina ◽  
Ivan Nikolayevich Romanov ◽  
Alexander Vladimirovich Shakhov ◽  
...  
Keyword(s):  
Small Intestine ◽  
Wall Thickness ◽  
Bowel Disease ◽  
Mesenteric Artery ◽  
Mesenteric Ischemia ◽  
Intestinal Wall ◽  
Ischemic Bowel ◽  
Acute Occlusion ◽  
Ischemic Bowel Disease ◽  
Ischemic Intestine

Relevance. Lethality rates (63-95%) among patients with acute intestinal ischemia remain consistently high during the last decades. Despite the high urgency of the problem and numerous works devoted to visualization, early laboratory and instrumental diagnostics of ischemic bowel disease, clinical results have not changed significantly during the last 15-20 years. Pathogenetic mechanisms of various variants of ischemic intestinal lesion and their pathomorphological manifestations should become the basis for the development of surgical tactics and require specification. The aim is to study pathomorphological manifestations and to clarify on their basis the pathogenetic mechanisms of ischemic involvement of the small intestine in acute occlusion of the mesenteric artery in the experiment. Materials and methods. An experimental study was performed on 10 laboratory animals - male rats of the Wistar line. Modeling acute mesenteric ischemia was carried out via dressing of the jejunal artery. The ischemic intestine was collected for a stepwise histological study, the morphometry of the layers of the intestinal wall and the prevalence of necrosis. Results. At the time of the appearance of macroscopic signs of lack of vitality in the central sector of the ischemic intestine, the wall thickness was 47.6% less than normal and in the adjacent peripheral sectors by 40.6%. Thinning of the intestinal wall was due to alteration of the mucosa with a decrease in its share in the total wall thickness from 86 to 82% (p = 0.021) in all sectors. The share of area occupied by necrosis in groups "B" and "C" was not statistically significant (p = 0.872) and amounted to 17.1 [7.3; 64.9]%. The state of the intramural vessels of the intestinal wall had significantly changed in comparison with the intact intestine. Signs of hypoperfusion in the form of sludge in the microvessels of the mucous membrane and the submucosa had been revealed. Conclusions. Pathomorphological manifestations of ischemic bowel disease in acute occlusive mesenteric ischemia resulted in a decrease in the total thickness of the wall and a change in the ratio of serous-muscular and mucosal-submucosal layers due to a decrease in the proportion of the latter. The severity of necrosis in the central and peripheral parts of the ischemic intestine did not differ.

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