Electrical activity and insulin release in pancreatic beta cells

1988 ◽  
Vol 90 (1-2) ◽  
pp. 127-138 ◽  
Author(s):  
Joel Keizer
Keyword(s):  
Electrical Activity ◽  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells

Membrane and intracellular effects of adenosine in mouse pancreatic beta-cells

1989 ◽  
Vol 257 (4) ◽  
pp. E473-E478 ◽  
Author(s):  
G. Bertrand ◽  
P. Petit ◽  
M. Bozem ◽  
J. C. Henquin
Keyword(s):  
Electrical Activity ◽  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells ◽  
Cell Function ◽  
Islet Cells ◽  
Pancreatic Beta Cell ◽  
Nucleoside Transport ◽  
High Concentration ◽  
Pancreatic Beta Cell Function

Mouse islets were used to study the effects of adenosine and its stable analogue L-N6-phenylisopropyladenosine (L-PIA) on pancreatic beta-cell function. At a high concentration (500 microM), adenosine augmented glucose-induced electrical activity in beta-cells and potentiated insulin release. These effects were prevented by the inhibitor of nucleoside transport nitrobenzylthioguanosine. They probably result from the metabolism of adenosine by beta-cells. At a lower concentration (50 microM), adenosine caused a small and transient inhibition of glucose-induced electrical activity and insulin release. L-PIA (10 microM) slightly and transiently inhibited insulin release, 45Ca efflux and 86Rb efflux from islet cells, and decreased electrical activity in beta-cells. When adenylate cyclase was stimulated by forskolin in the presence of 15 mM glucose, insulin release was strongly augmented. Under these conditions, L-PIA and adenosine (with nitrobenzylthioguanosine) caused a sustained inhibition. No such inhibition was observed when insulin release was potentiated by dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP). These data are consistent with the existence of A1 purinergic receptors on mouse beta-cells. They could mainly serve to attenuate the amplification of insulin release brought about by agents acting via cAMP.

Tetraethylammonium modifies gap junctions between pancreatic beta-cells

1981 ◽  
Vol 240 (3) ◽  
pp. C116-C120 ◽  
Author(s):  
M. S. Sheppard ◽  
P. Meda
Keyword(s):  
Beta Cell ◽  
Gap Junctions ◽  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells ◽  
Freeze Fracture ◽  
Potassium Conductance ◽  
Median Number ◽  
Rat Islets Of Langerhans ◽  
Gap Junctional

Gap junctions between pancreatic beta-cells were quantitatively assessed in freeze-fracture replicas of isolated rat islets of Langerhans incubated for 90 min with or without the potassium conductance blocker tetraethylammonium (TEA). The results show that TEA increases the median number of particles per beta-cell gap junction but not the frequency of gap junctions at both nonstimulating and threshold-stimulating concentrations of glucose. TEA increased the relative gap junctional area at both concentrations of glucose. TEA had no effect on insulin release at a basal concentration of glucose but potentiated that release at the threshold glucose level. Thus TEA modifies beta-cell gap junctions independently of its effect on insulin release. However, the junctional changes observed were greater when insulin release was also elevated.

2089268 Ultrasound Stimulation of Insulin Release From Pancreatic Beta Cells

2015 ◽  
Vol 41 (4) ◽  
pp. S65 ◽  
Author(s):  
Ivan Mauricio Suarez Castellanos ◽  
Aleksandar Jeremic ◽  
Vesna Zderic
Keyword(s):  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells ◽  
Ultrasound Stimulation ◽  
Stimulation Of

scholarly journals Ablation of AMP-activated protein kinase α1 and α2 from mouse pancreatic beta cells and RIP2.Cre neurons suppresses insulin release in vivo

Diabetologia ◽  
2010 ◽  
Vol 53 (5) ◽  
pp. 924-936 ◽  
Author(s):  
G. Sun ◽  
A. I. Tarasov ◽  
J. McGinty ◽  
A. McDonald ◽  
G. da Silva Xavier ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells ◽  
Amp Activated Protein Kinase

scholarly journals Glucose induces oscillatory Ca2+ signalling and insulin release in human pancreatic beta cells

Diabetologia ◽  
1994 ◽  
Vol 37 (S2) ◽  
pp. S11-S20 ◽  
Author(s):  
B. Hellman ◽  
E. Gylfe ◽  
P. Bergsten ◽  
E. Grapengiesser ◽  
P. E. Lund ◽  
...  
Keyword(s):  
Beta Cells ◽  
Insulin Release ◽  
Pancreatic Beta Cells
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