The effects of putative DNA repair inhibitors on DNA adduct levels and unscheduled DNA synthesis in rat hepatocytes exposed to 2-acetylaminofluorene

1989 ◽  
Vol 216 (1) ◽  
pp. 35-42 ◽  
Author(s):  
D. Jutras ◽  
M. Marion ◽  
F. Denizeau
Keyword(s):  
Dna Repair ◽  
Dna Synthesis ◽  
Rat Hepatocytes ◽  
Dna Adduct ◽  
Unscheduled Dna Synthesis ◽  
Dna Repair Inhibitors

An in vitro and an in vivo unscheduled DNA synthesis assay with a zinc oxide/ hexachloroethane (Zn/HCE) smoke

1996 ◽  
Vol 15 (1) ◽  
pp. 38-44 ◽  
Author(s):  
D. Andersonl ◽  
SD Blowers ◽  
TC Marrs ◽  
P. Rice
Keyword(s):  
Dna Repair ◽  
Dna Synthesis ◽  
Health Hazard ◽  
Micronucleus Assay ◽  
Zinc Content ◽  
Rat Hepatocytes ◽  
Unscheduled Dna Synthesis ◽  
In Vitro Investigation

1 Since Zn/HCE smoke has been shown previously to be weakly positive in the Ames test, and negative in the bone marrow micronucleus assay, other assays including a second in vivo assay examining unscheduled DNA synthesis (UDS) in rat hepatocytes has been carried out, as recommended by the UK Department of Health guide lines. 2 Zn/HCE smoke was assessed for its ability to induce DNA repair in an UDS assay both in vitro in cultured rat hepatocytes and in rat hepatocytes after in vivo treatment by inhalation. 3 For the in vitro investigation, two studies were carried out assessing media exposed to Zn/HCE smoke using at least seven concentrations up to a toxic level. At the highest concentration of Zn/HCE smoke, where some viable cells were seen, an increase in UDS was observed in both experiments. However this was not statistcally significant, was only seen at a level where toxicity was observed and was therefore considered not to be biologically significant. 4 In the in vivo investigation, one study was carried out in three separate parts, assessing two doses of Zn/HCE smoke characterised by their zinc content as approxi mately 20 and 56 μg l -1 air. A dose-related increase in UDS was observed which was not statistically sig nificant. The positive control behaved as anticipated, showing a highly statistically significant response. 5 It was concluded that Zn/HCE smoke did not induce unscheduled DNA repair in the in vitro or in vivo UDS assays under the conditions used in the studies. The overall lack of genotoxic effect of this smoke in this and previous studies in this laboratory would not suggest a major health hazard.

Reduction of nitromin to nitrogen mustard: unscheduled DNA synthesis in aerobic or anaerobic rat hepatocytes, JB1, BL8 and Walker carcinoma cell lines

1989 ◽  
Vol 10 (11) ◽  
pp. 2113-2118 ◽  
Author(s):  
Ian N. H. White ◽  
Mohammad Suzanger ◽  
A. Robin Mattocks ◽  
Eric Bailey ◽  
Peter B. Farmer ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Cell Lines ◽  
Nitrogen Mustard ◽  
Carcinoma Cell ◽  
Rat Hepatocytes ◽  
Unscheduled Dna Synthesis ◽  
Carcinoma Cell Lines ◽  
Walker Carcinoma

Modulation of chemical carcinogen-induced unscheduled DNA synthesis by dehydroepiandrosterone (DHEA) in the primary rat hepatocytes

1999 ◽  
Vol 22 (5) ◽  
pp. 474-478 ◽  
Author(s):  
Seung Hee Kim ◽  
Hyung-Mee Han ◽  
Seog Youn Kang ◽  
Ki Kyung Jung ◽  
Tae Gyun Kim ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Rat Hepatocytes ◽  
Unscheduled Dna Synthesis ◽  
Chemical Carcinogen ◽  
Primary Rat Hepatocytes
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