An experimental verification of the theory of diffusion limitation of immobilized enzymes

1982 ◽  
Vol 705 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Joachim Müller ◽  
Thomas Zwing
Keyword(s):  
Experimental Verification ◽  
Immobilized Enzymes ◽  
Diffusion Limitation

Modeling Trienzyme Biosensor at Internal Diffusion Limitation

2004 ◽  
Vol 9 (2) ◽  
pp. 139-144 ◽  
Author(s):  
J. Kulys
Keyword(s):  
Immobilized Enzymes ◽  
Internal Diffusion ◽  
Significant Response ◽  
Diffusion Limitation ◽  
Enzyme Reactions ◽  
Substrate Conversion ◽  
Apparent Stability ◽  
Biosensor Response

A model of biosensor containing three immobilized enzymes utilizing consecutive substrate conversion in the chain was developed. The modeling was performed at an internal diffusion limitation and a steadystate condition. The calculations showed that significant response of biosensors was produced if diffusion modules were larger than 1 for all enzyme reactions. Due to diffusion limitation the apparent stability of biosensor response increased many times in comparison to stability of the most labile enzyme of the chain.

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