The effect of demineralized bone powder on osteoblast proliferation and alkaline phosphatase incorporation in tissue culture

1987 ◽  
Vol 45 (11) ◽  
pp. M8-M9
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Osteoblast Proliferation ◽  
Bone Powder ◽  
Demineralized Bone

Studies on the Mechanism of Substrate Induction and L-Cyst(e)ine Repression of Alkaline Phosphatase in Mammalian Cell Cultures

1967 ◽  
Vol 2 (4) ◽  
pp. 545-555
Author(s):  
M. J. GRIFFIN ◽  
R. P. COX
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Cell Cultures ◽  
Phosphate Esters ◽  
Kidney Cell Line ◽  
Monkey Kidney Cell ◽  
African Green Monkey Kidney ◽  
Mammalian Cell Cultures ◽  
Green Monkey ◽  
Substrate Induction

The mechanisms of substrate induction and L-cyst(e)ine repression of alkaline phosphatase were studied in tissue culture using an established African green monkey kidney cell line (BS-C-I). L-Cyst(e)ine repression and substrate induction are mutually antagonistic. Evidence is presented which suggests that the increase in alkaline phosphatase levels induced by mono-phosphate esters may in part be due to protection of the enzyme from cellular degradation, while L-cyst(e)ine is believed to act either by repressing the synthesis of the enzyme or by selectively increasing its catabolism.

Distribution of Alkaline Phosphatase Variants in a Heteroploid Strain of Human Cells in Tissue Culture

Nature ◽  
1962 ◽  
Vol 196 (4854) ◽  
pp. 600-601 ◽  
Author(s):  
JOSEPH J. MAIO ◽  
LUIGI L. DE CARLI
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Human Cells

USING DEMINERALIZED BONE POWDER TO REPLACE FUNCTIONAL DEFICITS OF BONE

1993 ◽  
Vol 86 (Supplement) ◽  
pp. 109
Author(s):  
Matthew Concannon ◽  
Mark Boschert ◽  
Charles Puckett
Keyword(s):  
Functional Deficits ◽  
Bone Powder ◽  
Demineralized Bone

scholarly journals ON THE MECHANISMS OF BONE RESORPTION

1968 ◽  
Vol 39 (3) ◽  
pp. 676-697 ◽  
Author(s):  
Gilbert Vaes
Keyword(s):  
Tissue Culture ◽  
Alkaline Phosphatase ◽  
Protein Synthesis ◽  
Bone Resorption ◽  
Bone Mineral ◽  
Lysosomal Enzymes ◽  
Organic Matrix ◽  
Acid Hydrolases ◽  
New Synthesis ◽  
Stimulation Of

Bone resorption, characterized by the solubilization of both the mineral and the organic components of the osseous matrix, was obtained in tissue culture under the action of parathyroid hormone (PTH). It was accompanied by the excretion of six lysosomal acid hydrolases, which was in good correlation with the progress of the resorption evaluated by the release of phosphate, calcium 45 or hydroxyproline from the explants; there was no increased excretion of two nonlysosomal enzymes, alkaline phosphatase, and catalase. Balance studies and experiments with inhibitors of protein synthesis indicated that the intracellular stores of the acid hydrolases excreted were maintained by new synthesis. The release was not due to a direct disruption of the lysosomal membrane by PTH; it is presumed to result from an exocytosis of the whole lysosomal content and to involve mechanisms similar to those controlling the secretion of this content into digestive vacuoles. The resorbing explants acidified their culture fluids at a faster rate and released more lactate and citrate than the controls; this release was in good correlation, in the PTH-treated cultures, with the resorption of the bone mineral, but the amount of citrate released was considerably smaller than that of lactate. The acid released could account for the resorption of the mineral. It is proposed, as a working hypothesis, that the acid hydrolases of the lysosomes are active in the resorption of the organic matrix of bone and that acid, originating possibly from the stimulation of glycolysis, cares for the concomitant solubilization of bone mineral while also favoring the hydrolytic action of the lysosomal enzymes.

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