HPLC determination of serum ganciclovir using ultrafiltration, ultraviolet and fluorescence detection

1994 ◽  
Vol 12 (3) ◽  
pp. 429-432 ◽  
Author(s):  
Marlies Koel ◽  
Peter Nebinger
Keyword(s):  
Fluorescence Detection ◽  
Hplc Determination

A new method for the HPLC determination of gamma-hydroxybutyric acid (GHB) following derivatization with a coumarin analogue and fluorescence detection

Talanta ◽  
2008 ◽  
Vol 75 (2) ◽  
pp. 356-361 ◽  
Author(s):  
Constantinos K. Zacharis ◽  
Nikolaos Raikos ◽  
Nikolaos Giouvalakis ◽  
Helen Tsoukali-Papadopoulou ◽  
Georgios A. Theodoridis
Keyword(s):  
Fluorescence Detection ◽  
New Method ◽  
Hplc Determination ◽  
Hydroxybutyric Acid

scholarly journals HPLC determination of perfluorinated carboxylic acids with fluorescence detection

2010 ◽  
Vol 172 (3-4) ◽  
pp. 409-417 ◽  
Author(s):  
Ewa Poboży ◽  
Edyta Król ◽  
Lena Wójcik ◽  
Mariusz Wachowicz ◽  
Marek Trojanowicz
Keyword(s):  
Carboxylic Acids ◽  
Fluorescence Detection ◽  
Hplc Determination ◽  
Perfluorinated Carboxylic Acids

scholarly journals High-Performance Liquid Chromatographic Determination of Dihydroergocristine in a Pharmaceutical Formulation with Fluorescence Detection

2010 ◽  
Vol 93 (1) ◽  
pp. 97-101
Author(s):  
Michal Douša ◽  
Michaela Dubovská
Keyword(s):  
Fluorescence Detection ◽  
Mobile Phase ◽  
High Performance ◽  
Pharmaceutical Preparation ◽  
Potassium Dihydrogen Phosphate ◽  
Sample Pretreatment ◽  
Chromatographic Determination ◽  
Hplc Determination ◽  
Dihydrogen Phosphate

Abstract A rapid procedure based on a direct extraction and HPLC determination of dihydroergocristine in a pharmaceutical preparation with fluorescence detection has been developed and validated. The optimized chromatographic conditions included a Purospher RP18e column, 5 µm particle size, 250 4.0 mm, and 25 mM potassium dihydrogen phosphate buffer (pH 2.8)acetonitrile (60 + 40, v/v) mobile phase at a flow rate of 1 mL/min. The separation was carried out at 50C, and the injection volume was 5 L. Fluorescence detection was performed at an excitation and emission wavelength of 224 and 344 nm, respectively. The mobile phase parameters such as organic solvent composition, temperature, and pH were studied. The proposed method has the advantages of a very simple sample pretreatment and fast HPLC determination.

A new selective pre-column ninhydrin-based derivatization for a RP-HPLC determination of plasma asymmetric dimethyl-l-arginine (ADMA) by fluorescence detection

Amino Acids ◽  
2007 ◽  
Vol 34 (4) ◽  
pp. 677-682 ◽  
Author(s):  
Salvatore Sotgia ◽  
Angelo Zinellu ◽  
Gerard Aime Pinna ◽  
Luca Deiana ◽  
Ciriaco Carru
Keyword(s):  
Fluorescence Detection ◽  
Hplc Determination ◽  
Rp Hplc
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