The Role of Mineral Salts

Author(s):  
J.B. Finean
Keyword(s):  
1958 ◽  
pp. 285-303
Author(s):  
ARNE ENGSTRÖM ◽  
J.B. FINEAN
Keyword(s):  

1966 ◽  
Vol 12 (5) ◽  
pp. 915-931 ◽  
Author(s):  
L. C. Vining ◽  
P. M. Nair

The role of major nutrients in a glucose – mannitol – ammonium succinate – mineral salts medium which supports high production of clavine alkaloids was examined. Mannitol was found to be superior to other carbohydrates as a source of carbon but, under certain conditions, could be partially replaced with glucose. Ammonia provided the most suitable source of nitrogen, and was best supplied as ammonium succinate. In media with glucose and mannitol as principal carbon source, ammonium and succinate ions were utilized at parallel rates, maintaining the pH of the culture within the favorable range of 5.5–6.5 during alkaloid formation. For maximum yields there were optimum values for the initial concentrations of ammonia and phosphate in the medium. The concentration of phosphate required was growth-limiting. A high carbon:nitrogen ratio was necessary to prolong the phase of alkaloid synthesis. Production was increased by the "addition of L-tryptophan but not DL-mevalonic acid. The advantages of this medium for promoting alkaloid formation appear to be limited to clavine-producing strains isolated from Pennisetum typhoideum.


1927 ◽  
Vol 4 (10) ◽  
pp. 1269
Author(s):  
Henry A. Mattill
Keyword(s):  

2019 ◽  
Author(s):  
Ramin Fallahzadeh ◽  
Bijan Bambai ◽  
Nasrin Kamali ◽  
Kasra Esfahani ◽  
Abbas Akhavan Sepahi

AbstractThe sulfur contents of fossil fuels has negative impacts on the environment and human health. The bio-catalytic desulfurization strategies and the biological refinement of the fossil fuels are a cost-effective process compared to chemical desulfurization. Rhodococcus erythropolis IGTS8 is able to extract the organic sulfur of oil as mineral salts by 4S metabolic pathway (i.e. DszA,B,C and D genes). dszD gene codes a NADH:FMN oxidoreductase delivering reducing equivalent to DszA, DszB, and DszC to remove sulfur from heterocyclic molecules. In this study, we sought to improve DszD specificity by side-direct mutagenesis based on prediction of the DszD structure, molecular docking, and molecular dynamic simulation. Accordingly, the cloning, expression and activity assay of best candidates (A79I and A79N) were performed. Our results demonstrated the important role of position 79 in enzyme activity, the A79I and A79N mutants are able to increase the enzyme activity 3.4 and 5.2 fold compared to wild-type.


2009 ◽  
Vol 192 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Fumiko Taguchi ◽  
Tomoko Suzuki ◽  
Yoshishige Inagaki ◽  
Kazuhiro Toyoda ◽  
Tomonori Shiraishi ◽  
...  

ABSTRACT To investigate the role of iron uptake mediated by the siderophore pyoverdine in the virulence of the plant pathogen Pseudomonas syringae pv. tabaci 6605, three predicted pyoverdine synthesis-related genes, pvdJ, pvdL, and fpvA, were mutated. The pvdJ, pvdL, and fpvA genes encode the pyoverdine side chain peptide synthetase III l-Thr-l-Ser component, the pyoverdine chromophore synthetase, and the TonB-dependent ferripyoverdine receptor, respectively. The ΔpvdJ and ΔpvdL mutants were unable to produce pyoverdine in mineral salts-glucose medium, which was used for the iron-depleted condition. Furthermore, the ΔpvdJ and ΔpvdL mutants showed lower abilities to produce tabtoxin, extracellular polysaccharide, and acyl homoserine lactones (AHLs), which are quorum-sensing molecules, and consequently had reduced virulence on host tobacco plants. In contrast, all of the mutants had accelerated swarming ability and increased biosurfactant production, suggesting that swarming motility and biosurfactant production might be negatively controlled by pyoverdine. Scanning electron micrographs of the surfaces of tobacco leaves inoculated with the mutant strains revealed only small amounts of extracellular polymeric matrix around these mutants, indicating disruption of the mature biofilm. Tolerance to antibiotics was drastically increased for the ΔpvdL mutant, as for the ΔpsyI mutant, which is defective in AHL production. These results demonstrated that pyoverdine synthesis and the quorum-sensing system of Pseudomonas syringae pv. tabaci 6605 are indispensable for virulence in host tobacco infection and that AHL may negatively regulate tolerance to antibiotics.


JAMA ◽  
1966 ◽  
Vol 195 (12) ◽  
pp. 1005-1009 ◽  
Author(s):  
D. J. Fernbach
Keyword(s):  

2018 ◽  
Vol 41 ◽  
Author(s):  
Winnifred R. Louis ◽  
Craig McGarty ◽  
Emma F. Thomas ◽  
Catherine E. Amiot ◽  
Fathali M. Moghaddam

AbstractWhitehouse adapts insights from evolutionary anthropology to interpret extreme self-sacrifice through the concept of identity fusion. The model neglects the role of normative systems in shaping behaviors, especially in relation to violent extremism. In peaceful groups, increasing fusion will actually decrease extremism. Groups collectively appraise threats and opportunities, actively debate action options, and rarely choose violence toward self or others.


2018 ◽  
Vol 41 ◽  
Author(s):  
Kevin Arceneaux

AbstractIntuitions guide decision-making, and looking to the evolutionary history of humans illuminates why some behavioral responses are more intuitive than others. Yet a place remains for cognitive processes to second-guess intuitive responses – that is, to be reflective – and individual differences abound in automatic, intuitive processing as well.


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