Comparative monitoring of temporal and spatial changes in tree water status using the non-invasive leaf patch clamp pressure probe and the pressure bomb

2010 ◽  
Vol 98 (2) ◽  
pp. 283-290 ◽  
Author(s):  
S. Rüger ◽  
W. Ehrenberger ◽  
M. Arend ◽  
P. Geßner ◽  
G. Zimmermann ◽  
...  
Keyword(s):  
Patch Clamp ◽  
Water Status ◽  
Pressure Probe ◽  
Non Invasive ◽  
Spatial Changes ◽  
Pressure Bomb ◽  
Temporal And Spatial

Expression of oncofetal fibronectin in porcine conceptuses and uterus throughout gestation

1996 ◽  
Vol 8 (8) ◽  
pp. 1207 ◽  
Author(s):  
W Tuo ◽  
FW Bazer
Keyword(s):  
Monoclonal Antibody ◽  
Animal Model ◽  
Epithelial Cells ◽  
Mammalian Species ◽  
Normal Pregnancy ◽  
Non Invasive ◽  
Spatial Changes ◽  
Porcine Conceptus ◽  
Oncofetal Fibronectin ◽  
Temporal And Spatial

Oncofetal fibronectin is reportedly expressed specifically by trophoblast tissue and some tumours and speculated to mediate placental attachment. In the present study, a monoclonal antibody (FDC-6) to human oncofetal fibronectin was used to characterize temporal and spatial changes in the expression of oncofetal fibronectin at the fetal-maternal interface of pigs. Conceptus and uterine tissues were collected from gilts throughout normal pregnancy and processed for immunohistochemical characterization. Results indicated that oncofetal fibronectin was constitutively expressed by both porcine conceptus and uterus throughout gestation. The most abundant staining for oncofetal fibronectin was found in the allantochorion and detectable levels of oncofetal fibronectin were also detected in luminal and glandular epithelial cells in the uterus. During the second-half of pregnancy, oncofetal fibronectin was also detected in fibroblast-like cells in the uterine stroma, but not in the stroma of the allantochorion. Owing to the non-invasive nature of the porcine placenta, the abundant expression of oncofetal fibronectin by the trophoblast and uterus may influence attachment between chorion and endometrium during pregnancy. Since attachment is the first step in implantation and placentation in all mammalian species, the pig may represent an excellent animal model to study interactions between trophoblast and endometrium mediated by oncofetal fibronectin.

Sparse Representation for Cardiac Electrical Activity Imaging in Magnetocardiography

2021 ◽  
Vol 11 (7) ◽  
pp. 2025-2032
Author(s):  
Lu Bing ◽  
Wei Wang
Keyword(s):  
Magnetic Field ◽  
Electrical Activity ◽  
Current Source ◽  
Optimization Methods ◽  
Sparse Constraint ◽  
Non Invasive ◽  
Cardiac Electrical Activity ◽  
Underdetermined System ◽  
Spatial Changes ◽  
Temporal And Spatial

Signal sparsity has been widely discussed in communication system, cloud computing, multimedia processing and computational biology. Reconstructing the sparsely distributed current sources of the heart by means of non-invasive magnetocardiography (MCG) measurement and various optimization methods provides a new way to solve the inverse problem of the cardiac magnetic field. The problem of sparse source location of MCG is in the time series of MCG measurement caused by active sparse current source, can the spatiotemporal source be reconstructed accurately and effectively? For the above problem, the scientific contributions of the paper include: (1) A modified focal underdetermined system solver algorithm is proposed for a sparse solution, by combing with dynamic regularization factor and smoothed sparse constraint; (2) Lead field matrix is reduced by prior information of cardiac magnetic field map to reduce under-determination; (3) Spatiotemporal sources are reconstructed for non-invasive cardiac electrical activity imaging. The results of real MCG data demonstrate the effectiveness of this method for cardiac electrical activity imaging. The temporal and spatial changes of the current sources are similar to the depolarization and repolarization process of the ventricle.

scholarly journals Temporal and spatial changes in wall shear stress during atherosclerotic plaque progression in mice

2018 ◽  
Vol 5 (3) ◽  
pp. 171447 ◽  
Author(s):  
R. Xing ◽  
A. M. Moerman ◽  
Y. Ridwan ◽  
M. J. Daemen ◽  
A. F. W. van der Steen ◽  
...  
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Mouse Model ◽  
Atherosclerotic Plaque ◽  
Proximal Part ◽  
Plaque Progression ◽  
Plaque Composition ◽  
Spatial Changes ◽  
Temporal And Spatial ◽  
Wall Shear

Wall shear stress (WSS) is involved in atherosclerotic plaque initiation, yet its role in plaque progression remains unclear. We aimed to study (i) the temporal and spatial changes in WSS over a growing plaque and (ii) the correlation between WSS and plaque composition, using animal-specific data in an atherosclerotic mouse model. Tapered casts were placed around the right common carotid arteries (RCCA) of ApoE −/− mice. At 5, 7 and 9 weeks after cast placement, RCCA geometry was reconstructed using contrast-enhanced micro-CT. Lumen narrowing was observed in all mice, indicating the progression of a lumen intruding plaque. Next, we determined the flow rate in the RCCA of each mouse using Doppler Ultrasound and computed WSS at all time points. Over time, as the plaque developed and further intruded into the lumen, absolute WSS significantly decreased. Finally at week 9, plaque composition was histologically characterized. The proximal part of the plaque was small and eccentric, exposed to relatively lower WSS. Close to the cast a larger and concentric plaque was present, exposed to relatively higher WSS. Lower WSS was significantly correlated to the accumulation of macrophages in the eccentric plaque. When pooling data of all animals, correlation between WSS and plaque composition was weak and no longer statistically significant. In conclusion, our data showed that in our mouse model absolute WSS strikingly decreased during disease progression, which was significantly correlated to plaque area and macrophage content. Besides, our study demonstrates the necessity to analyse individual animals and plaques when studying correlations between WSS and plaque composition.

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