scholarly journals Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweed Eucheuma denticulatum

2022 ◽  
Vol 62 ◽  
pp. 102619
Author(s):  
Simon Gregersen ◽  
Margarita Pertseva ◽  
Paolo Marcatili ◽  
Susan Løvstad Holdt ◽  
Charlotte Jacobsen ◽  
...  
Keyword(s):  
Pilot Scale ◽  
Hot Water ◽  
Red Seaweed ◽  
Eucheuma Denticulatum ◽  
Water Extracts

scholarly journals Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweed Eucheuma denticulatum

2020 ◽  
Author(s):  
Simon Gregersen ◽  
Margarita Pertseva ◽  
Paolo Marcatili ◽  
Susan Løvstad Holdt ◽  
Charlotte Jacobsen ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
De Novo ◽  
Protein Extraction ◽  
Transcriptome Assembly ◽  
Protein Composition ◽  
Pilot Scale ◽  
Extracellular Proteins ◽  
Hot Water ◽  
Label Free

AbstractSeaweeds have a long history as a resource for polysaccharides/hydrocolloids extraction for use in the food industry due to their functionality as stabilizing agents. In addition to the carbohydrate content, seaweeds also contains a significant amount of protein, which may find application in food and feed. Here, we present a novel combination of transcriptomics, proteomics, and bioinformatics to determine the protein composition in two pilot-scale extracts from Eucheuma denticilatum (Spinosum) obtained via hot-water extraction. The extracts were characterized by qualitative and quantitative proteomics using LC-MS/MS and a de-novo transcriptome assembly for construction of a novel proteome. Using label-free, relative quantification, we were able to identify the most abundant proteins in the extracts and determined that the majority of quantified protein in the extracts (>75%) is constituted by merely three previously uncharacterized proteins. Putative subcellular localization for the quantified proteins was determined by bioinformatic prediction, and by correlating with the expected copy number from the transcriptome analysis, we determined that the extracts were highly enriched in extracellular proteins. This implies that the method predominantly extracts extracellular proteins, and thus appear ineffective for cellular disruption and subsequent release of intracellular proteins. Ultimately, this study highlight the power of quantitative proteomics as a novel tool for characterization of alternative protein sources intended for use in foods. Additionally, the study showcases the potential of proteomics for evaluation of protein extraction methods and as powerful tool in the development of an efficient extraction process.

Characterization of pressurized hot water extracts of grape pomace: Chemical and biological antioxidant activity

2015 ◽  
Vol 171 ◽  
pp. 62-69 ◽  
Author(s):  
J.R. Vergara-Salinas ◽  
Mauricio Vergara ◽  
Claudia Altamirano ◽  
Álvaro Gonzalez ◽  
J.R. Pérez-Correa
Keyword(s):  
Antioxidant Activity ◽  
Grape Pomace ◽  
Hot Water ◽  
Water Extracts

scholarly journals Characterization of Mitogenic Substances in the Hot Water Extracts of Bupleuri Radix.

1995 ◽  
Vol 18 (5) ◽  
pp. 757-765 ◽  
Author(s):  
Hideki OKA ◽  
Naohito OHNO ◽  
Shogo IWANAGA ◽  
Shigeru IZUMI ◽  
Takuya KAWAKITA ◽  
...  
Keyword(s):  
Hot Water ◽  
Water Extracts ◽  
Bupleuri Radix

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

Evaluation on the Muscular Strength Activity of Medicinal Herb Hot-Water Extracts

2007 ◽  
Vol 36 (6) ◽  
pp. 678-682 ◽  
Author(s):  
Chang-Ho Lee ◽  
Young-Eon Kim ◽  
In-Ho Kim ◽  
Dae-Seok Han ◽  
Ki-Seung Seong ◽  
...  
Keyword(s):  
Muscular Strength ◽  
Medicinal Herb ◽  
Hot Water ◽  
Water Extracts
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