Corrigendum to “Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Inês ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin” [Anim. Reprod. Sci. 203 (2019) 25–32]

Antral follicle count (AFC) performed after an oestrus synchronization protocol has been studied as a tool to select ewes with high potential for invivo embryo production (Pinto et al. 2018 Theriogenology 113, 146-152). However, it would be interesting to know whether AFC assessed on a random day of the oestrous cycle correlates with the superovulatory response. The present study was conducted to evaluate the correlation between AFC at the beginning of progesterone (P4)-based oestrus synchronization protocol used as basis of superovulatory treatment and the number of corpora lutea (CL) 12h before recovery of embryos in Santa Inês ewes. The study was conducted during September and October in northeast Brazil (03°40′26″S and 40°14′20″W) using 8 adult Santa Inês ewes. On a random day of oestrous cycle (Day 0) all ewes received an intravaginal device (CIDR) of progesterone (0.3g, Eazi-breed, Zoetis), which remained for 9 days. On Day 7, the pFSH (133mg, Folltropin V, Vetoquinol) treatment began, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) injected IM at 12-h intervals. On Day 9, 2 equal doses of D-cloprostenol were injected at a 12-h interval (37.5µg, Prolise, Agener União). All ewes showed oestrus and were mated by fertile rams. Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was administered IM on Days 12, 13, and 15. On Day 16, non-surgical embryo recovery (NSER) was performed after cervical dilation using D-cloprostenol and oestradiol benzoate at 16h and oxytocin 20min before. Transrectal B-mode ultrasound evaluations (Z5 Vet, Mindray), frequency 7.5MHz, were performed on Day 0 and 7 and 12h before NSER to evaluate the ovarian population present. Pearson correlation analysis (P<0.05) was performed using Bioestat 5.3 software. The number of AFC per ewe at the beginning of the protocol and on Day 7 were 9.9±2.7 and 11±3.2, respectively. The numbers of CL, recovered embryos, and viable embryos were 14.0±3.5, 8.2±10.9, and 6.0±11.0, respectively. There was no correlation of AFC on a random day of oestrous cycle with the number of AFC on Day 7 (P=0.42), number of corpora lutea (P=0.44), number of recovered embryos (P=0.18), or number of viable embryos (P=0.11) in superovulated ewes. In conclusion, we did not find significant correlations between AFC on a random day of oestrous cycle and the superovulatory/embryos response in Santa Inês ewes. Financial support for this study was provided by Embrapa (02.13.06.026.00.02 and 02.13.06.026.00.04) and FAPEMIG (PPM 00201-17).

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Embryo recovery rate in Santa Inês ewes subjected to successive superovulatory treatments with pFSH

Small Ruminant Research ◽

10.1016/s0921-4488(03)00059-2 ◽

2003 ◽

Vol 49 (1) ◽

pp. 19-23 ◽

Cited By ~ 12

Author(s):

M.F. Cordeiro ◽

J.B. Lima-Verde ◽

E.S. Lopes-Júnior ◽

D.I.A. Teixeira ◽

L.N. Farias ◽

...

Keyword(s):

Recovery Rate ◽

Embryo Recovery ◽

Santa Inês

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225 Evaluation of superovulatory response in Brazilian native sheep by B-mode ultrasonography

Reproduction Fertility and Development ◽

10.1071/rdv32n2ab225 ◽

2020 ◽

Vol 32 (2) ◽

pp. 240

Author(s):

G. B. Vergani ◽

M. S. D. Lima ◽

K. M. Silva ◽

A. W. U. Monteiro ◽

A. F. Ramos ◽

...

Keyword(s):

Recovery Rate ◽

Pearson Correlation ◽

Animal Health ◽

Device Removal ◽

Embryo Recovery ◽

Flunixin Meglumine ◽

Ultrasound Evaluation ◽

Release Device ◽

Linear Probe ◽

Santa Inês

The objective of this study was to evaluate the correlation between the number of corpora lutea (CL), as determined by B-mode ultrasonography, and the total number of structures recovery by non-surgical embryo recovery (NSER) from superovulated ewes. Somali (n=18), Santa Inês (n=18), and Morada Nova (n=16) ewes received an intravaginal device with progesterone (0.33g, controlled internal drug release device, CIDR, Zoetis), which was maintained for 9 days. The superovulatory protocol started 60h before device removal, with 6 decreasing doses (25, 25, 15, 15, 10, and 10%) of p-FSH (IM 133mg, Folltropin V, Vetoquinol) injected every 12h. D-Cloprostenol (IM 37.5μg, Prolise, Agener Union) was injected 12h before and at the moment of device removal. Ewes in oestrus were mated by fertile male rams three times after the onset of oestrus with a 12-h interval. Flunixin meglumine (24.9 mg; Banamine, MSD Animal Health) was injected IM on Days 12, 13, and 15. One day before NSER, B-mode ultrasound evaluations were performed using portable equipment (Z5 Vet, Mindray) with a stiffened multifrequency linear probe to evaluate the number of structures present in the ovaries. Embryo recovery was performed 7 days after progesterone CIDR removal (Day 16). The number of CL and the number of recovered structures were determined and their association evaluated using the Pearson correlation test (P<0.05). Only 17 Somali, 16 Santa Inês, and 15 Morada Nova ewes were submitted to NSER; the other 4 ewes could not be flushed because of low cervical dilation or cervical puncture. After B-mode ultrasound evaluation, 168, 217, and 131 CL were observed, with an average of 9.9 (range: 1 to 12), 13.5 (range: 2 to 47), and 8.7 (range: 3 to 18) CL per donor for Somali, Santa Inês, and Morada Nova ewes, respectively. The recovery rates (i.e. structures recovered by the number of CL counted) were 60.1% (101/168), 96.3% (209/217), and 103.5% (135/131) for the 3 breeds, respectively. There was a positive correlation (r=0.69; P<0.01) between the number of recovered structures and number of CL. Those findings confirm results from other studies, in which the recovery rate varied from 35 to 91%. In some cases, recovery rate can be overestimated because of the difficulty in quantifying CL. In conclusion, B-mode ultrasonography can be used to estimate superovulatory response in ewes and thus as a criterion to decide whether a donor will undergo embryo flushing. Financial support for this study was provided by Embrapa (02.13.06.026.00.04) and Fapemig (CVZ-PPM 00201-17).

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Comparison of the intravenous and intravaginal route of oxytocin administration for cervical dilation protocol and non‐surgical embryo recovery in oestrous‐induced Santa Inês ewes

Reproduction in Domestic Animals ◽

10.1111/rda.13499 ◽

2019 ◽

Vol 54 (9) ◽

pp. 1230-1235 ◽

Cited By ~ 4

Author(s):

Lucia Prellwitz ◽

Fabiana Nunes Zambrini ◽

José Domingos Guimarães ◽

Marco Antonio Paula de Sousa ◽

Maria Emília Franco Oliveira ◽

...

Keyword(s):

Cervical Dilation ◽

Embryo Recovery ◽

Santa Inês

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Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Inês ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin

Animal Reproduction Science ◽

10.1016/j.anireprosci.2019.02.004 ◽

2019 ◽

Vol 203 ◽

pp. 25-32 ◽

Cited By ~ 9

Author(s):

J.F. Fonseca ◽

F.N. Zambrini ◽

J.D. Guimarães ◽

M.R. Silva ◽

M.E.F. Oliveira ◽

...

Keyword(s):

Embryo Recovery ◽

Santa Inês

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Successive in vivo embryo production in Santa Inês sheep

Animal Production Science ◽

10.1071/an18740 ◽

2020 ◽

Vol 60 (4) ◽

pp. 497 ◽

Cited By ~ 4

Author(s):

Pedro Henrique Nicolau Pinto ◽

Mario Felipe Alvarez Balaro ◽

Helena Fabiana Reis de Almeida Saraiva ◽

Viviane Lopes Brair ◽

Vivian Angélico Pereira Alfradique ◽

...

Keyword(s):

Recovery Rate ◽

Doppler Ultrasonography ◽

Corpora Lutea ◽

Colour Doppler ◽

Embryo Production ◽

Previous Response ◽

Embryo Recovery ◽

Colour Doppler Ultrasonography ◽

Santa Inês

Context In vivo embryo production, also called multiple ovulation and embryo transfer, can accelerate genetic gain, and thus improve animal production. However, there are issues limiting a wider use of this biotechnology in sheep livestock. Aims This study aimed to determine (1) whether a previous response to superovulation (SOV) can be used as a criterion to select ewes for in vivo embryo production, (2) whether the intensity of the SOV response (number of corpora lutea, CL) can affect the embryo recovery rate, and (3) whether the number of CL quantified by colour Doppler ultrasonography can be used to calculate the recovery rate. Methods Twenty-five Santa Inês ewes underwent SOV three times (SOV1, SOV2 and SOV3), with 200 mg FSH and natural mating. The number of CL after each SOV was determined by laparoscopy and by colour Doppler ultrasonography. Key results The number of CL significantly decreased (P < 0.05) after SOV1 (7.5 ± 4.8) to 3.0 ± 5.0 at SOV 2 and 2.2 ± 3.5 at SOV3. Strong correlations were observed between SOV2 and SOV3 in terms of numbers of CL (r = 0.86, r2 = 0.74; P < 0.0001) and viable embryos (r = 0.79, r2 = 0.63; P < 00001). However, no correlations were observed between SOV1 and SOV2 or between SOV1 and SOV3. Recovery rate did not differ with the intensity of the SOV response (≤6, 7–10, >10 CL) or between the methods used to quantify CL. Conclusions Ewes did not show the same pattern of response when submitted to successive FSH-based SOV. The intensity of the SOV response did not affect the recovery rate, and the number of CL estimated by colour Doppler ultrasonography can be used to calculate the recovery rate. Implications Selecting sheep embryo donors by a previous SOV response is not always feasible. The recovery rate is homogeneous and it is not affected by the intensity of the SOV response. A nonsurgical technique can be used to assess the recovery rate, improving animal welfare in MOET programs.

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Relação Ômegas 6 e 3 em Ovinos da Raça Santa Inês Alimentados com Diferentes Níveis de Inclusão de Casca de Soja em Substituição ao Milho

Revista Científica de Produção Animal ◽

10.15528/2176-4158/rcpa.v13n1p111-114 ◽

2011 ◽

Vol 13 (1) ◽

pp. 111-114

Author(s):

J.I. Simionatto ◽

L.S. Costa ◽

R.R. Silva ◽

M.S. Macedo ◽

A.P. Lira ◽

...

Keyword(s):

Santa Inês

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Composição Tecidual e Medidas do Lombo de Cordeiros Santa Inês Recebendo Água com Diferentes Teores de Salinidade

Revista Científica de Produção Animal ◽

10.15528/2176-4158/rcpa.v14n2p227-230 ◽

2012 ◽

Vol 14 (2) ◽

pp. 227-230

Author(s):

D.P.V. Castro ◽

S.M. Yamamoto ◽

G.G.L. Araújo ◽

Í.R.R. Albuquerque ◽

J.H.A. Moura ◽

...

Keyword(s):

Santa Inês

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COMPORTAMENTO INGESTIVO DE OVINOS ALIMENTADOS COM TORTA DE GIRASSOL ORIUNDA DA PRODUÇÃO DE BIODIESEL

Revista Científica Rural ◽

10.30945/rcr-v21i1.334 ◽

2019 ◽

Vol 21 (1) ◽

pp. 179-187

Author(s):

Lidiane Fagundes da Silva Monteiro ◽

Elanne De Paiva Fonseca ◽

Aline Moreira Portella de Melo ◽

Allison Ferreira de Lima ◽

Valdi De Lima Júnior ◽

...

Keyword(s):

Santa Inês

O objetivo deste estudo foi avaliar os efeitos da inclusão de diferentes níveis da torta de girassol sobre o comportamento ingestivo de cordeiros. Utilizaram-se 36 cordeiros mestiços das raças Santa Inês x Dorper alojados individualmente, de forma aleatória, sendo 9 por tratamento, correspondentes ao tratamento controle (0%) e os demais com inclusões ao nível de 5, 10 e 15%. A avaliação do comportamento ingestivo consistiu no registro do tempo gasto com as atividades de ruminação, ócio e alimentação, mediante observação durante 24 horas. As observações comportamentais foram efetuadas em 3 períodos com intervalos de 15 dias. As variáveis estudadas foram submetidas à análise de regressão em nível de 5% de probabilidade. Para os tempos despendidos em ruminação, ócio e alimentação, não houve efeito significativo (P>0,05). Quanto ao período do dia, o maior tempo de ingestão de ração foi no período entre 8-14h e 14-20h. As maiores frequências de ruminação foram durante a madrugada (20-2h) e início da manhã (2-8h). Já de ócio foi entre 20-2h. É possível adicionar torta de girassol até 15% em dietas de ovinos sem comprometer o comportamento ingestivo, estando sob as condições climáticas submetidas.

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