The utility of immunohistochemical testing for mismatch repair proteins in fine needle aspiration specimens of pancreatic adenocarcinoma

2020 ◽  
Vol 47 ◽  
pp. 151552
Author(s):  
Daniel Mettman ◽  
Erin Haer ◽  
Mojtaba Olyaee ◽  
Amit Rastogi ◽  
Rashna Madan ◽  
...  
Keyword(s):  
Mismatch Repair ◽  
Pancreatic Adenocarcinoma ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Fine Needle ◽  
Mismatch Repair Proteins ◽  
Immunohistochemical Testing

The Utility of UroVysion Fluorescence In Situ Hybridization in Pancreatic Fine-Needle Aspiration Samples Directed and Obtained by Endoscopic Ultrasonography

2013 ◽  
Vol 137 (1) ◽  
pp. 64-71 ◽  
Author(s):  
David N. Henkes ◽  
Sandeep N. Patel ◽  
Laura A. Rosenkranz ◽  
Jose L. Escobedo
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Pancreatic Adenocarcinoma ◽  
Fine Needle Aspiration ◽  
False Negative ◽  
High Specificity ◽  
Needle Aspiration ◽  
Fine Needle

Context.—The diagnosis of pancreatic adenocarcinoma can be challenging for the pathologist. Endoscopic ultrasound-guided, fine-needle aspiration (EUS-FNA) can be used to obtain samples of pancreatic masses. UroVysion fluorescence in situ hybridization (UFISH) has been reported to increase the sensitivity and to be very specific for the diagnosis of adenocarcinoma when combined with cytology in the diagnosis of biliary brushings and washings. Objectives.—To determine the sensitivity and specificity of UFISH on tissues obtained from pancreatic lesions suggestive of adenocarcinoma obtained by EUS-FNA, compared against fine-needle aspiration (FNA) results. Additionally, to use patient follow-up data to evaluate UFISH results in FNA samples that showed significant atypia but did not meet the criteria for malignancy. Design.—Sixty consecutive cases of pancreatic EUS-FNA from our institution submitted for UFISH testing. Results.—Polysomic UFISH has a sensitivity of 93% and a specificity of 100% when compared against FNA results. Follow-up studies showed that adding UFISH to FNA increased the sensitivity for patients with true-positive results from 83% to 94% and increased specificity from 85% to 100%. For 7 patients with suspicious FNA results who had sufficient follow-up, UFISH was 100% sensitive and 100% specific. Conclusions.—UFISH can be used to confirm the diagnosis of malignancy in pancreatic adenocarcinoma. Because of the high specificity, polysomic UFISH may help establish a diagnosis of malignancy when the FNA features are suggestive of, but not conclusive for, malignancy. The most common cause for a false-negative UFISH result was insufficient numbers of malignant cells.

Sa1458 Synchronous Primary Pancreatic Adenocarcinoma, a Poorly Understood Phenomenon, Diagnosed by Endoscopic Ultrasound-Guided Fine Needle Aspiration

2016 ◽  
Vol 150 (4) ◽  
pp. S321
Author(s):  
Tarun Rustagi ◽  
Ferga Gleeson ◽  
Suresh Chari ◽  
Barham K. Abu Dayyeh ◽  
Michael B. Farnell ◽  
...  
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Endoscopic Ultrasound ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrasound Guided ◽  
Fine Needle

scholarly journals Hemorrhagic gastric ulcer after endoscopic ultrasound-guided fine needle aspiration of a pancreatic adenocarcinoma

Endoscopy ◽  
2015 ◽  
Vol 47 (S 01) ◽  
pp. E635-E636
Author(s):  
Tomoya Iida ◽  
Takeya Adachi ◽  
Suguru Nakagaki ◽  
Takashi Yabana ◽  
Akira Goto ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Pancreatic Adenocarcinoma ◽  
Endoscopic Ultrasound ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrasound Guided ◽  
Fine Needle

scholarly journals EUS – Fine- Needle Aspiration Biopsy (FNAB) in the Diagnosis of Pancreatic Adenocarcinoma: A Review

2016 ◽  
Vol 54 (1) ◽  
pp. 24-30 ◽  
Author(s):  
Alexandra Kalogeraki ◽  
Georgios Z. Papadakis ◽  
Dimitrios Tamiolakis ◽  
Iliana Karvela-Kalogeraki ◽  
Mihailos Karvelas-Kalogerakis ◽  
...  
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Malignant Neoplasms ◽  
Fine Needle ◽  
Tissue Diagnosis ◽  
Highly Sensitive ◽  
Needle Aspiration Biopsy

Solid masses of the pancreas represent a variety of benign and malignant neoplasms of the exocrine and endocrine tissues of the pancreas. A tissue diagnosis is often required to direct therapy in the face of uncertain diagnosis or if the patient is not a surgical candidate either due to advanced disease or comorbidities. Endoscopic ultrasound (EUS) is a relatively new technology that employs endoscopy and high-frequency ultrasound (US). EUS involves imaging of the pancreatic head and the uncinate from the duodenum and imaging of the body and tail from the stomach. It has been shown to be a highly sensitive method for the detection of pancreatic masses. It is superior to extracorporeal US and computed tomographic (CT) scans, especially when the pancreatic tumor is smaller than 2-3 cm. Although EUS is highly sensitive in detecting pancreatic solid masses, its ability to differentiate between inflammatory masses and malignant disease is limited. Endoscopic retrograde cholangiopancreatography (ERCP) brushing, CT-guided biopsies, and transabdominal ultrasound (US) have been the standard nonsurgical methods for obtaining a tissue diagnosis of pancreatic lesions, but a substantial false-negative rate has been reported. Transabdominal US-guided fine-needle aspiration biopsy (US-FNAB) has been used for tissue diagnosis in patients with suspected pancreatic carcinoma. It has been shown to be highly specific, with no false-positive diagnoses. With the advent of curvilinear echoendoscopes, transgastric and transduodenal EUS-FNAB of the pancreas have become a reality EUS with FNAB has revolutionized the ability to diagnose and stage cancers of the gastrointestinal tract and assess the pancreas. Gastrointestinal cancers can be looked at with EUS and their depth of penetration into the intestinal wall can be determined. Any suspicious appearing lymph nodes can be biopsied using EUS/FNAB. The pancreas is another organ that is well visualized with EUS. Abnormalities such as tumors and cysts of the pancreas can be carefully evaluated using EUS and then biopsied with FNAB. There are many new applications of EUS using FNAB. Researchers are looking to deliver chemotherapeutics into small pancreatic cancers and cysts. Nerve blocks using EUS/FNAB to inject numbing medicines into the celiac ganglia, a major nerve cluster, are now routinely performed in patients with pain due to pancreatic cancer. The aim of this study is to perform a review of the literature regarding the usefulness of EUS/FNAB in the diagnosis of pancreatic adenocarcinoma.

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