scholarly journals Construction of a genetic linkage map, QTLs mapping for low salinity and growth-related traits and identification of the candidate genes in Pacific white shrimp (Litopenaeus vannamei)

2022 ◽  
Vol 22 ◽  
pp. 100978
Author(s):  
Bing Chen ◽  
Ping Zhong ◽  
Xiaopeng Wu ◽  
Kai Peng ◽  
Yuping Sun ◽  
...  
2019 ◽  
Vol 10 ◽  
Author(s):  
Maoxian Huang ◽  
Yangfan Dong ◽  
Yan Zhang ◽  
Qinsheng Chen ◽  
Jia Xie ◽  
...  

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Guosong Zhang ◽  
Jie Li ◽  
Jiajia Zhang ◽  
Xia Liang ◽  
Tao Wang ◽  
...  

Abstract Background A high-density genetic linkage map is essential for QTL fine mapping, comparative genome analysis, identification of candidate genes and marker-assisted selection in aquaculture species. Pelteobagrus vachelli is a very popular commercial species in Asia. However, some specific characters hindered achievement of the traditional selective breeding based on phenotypes, such as lack of large-scale genomic resource and short of markers tightly associated with growth, sex determination and hypoxia tolerance related traits. Results By making use of 5059 ddRAD markers in P. vachelli, a high-resolution genetic linkage map was successfully constructed. The map’ length was 4047.01 cM by using an interval of 0.11 cm, which is an average marker standard. Comparative genome mapping revealed that a high proportion (83.2%) of markers with a one-to-one correspondence were observed between P. vachelli and P. fulvidraco. Based on the genetic map, 8 significant genome-wide QTLs for 4 weight, 1 body proportion, 2 sex determination, and 1 hypoxia tolerance related traits were detected on 4 LGs. Some SNPs from these significant genome-wide QTLs were observably associated with these phenotypic traits in other individuals by Kompetitive Allele Specific PCR. In addition, two candidate genes for weight, Sipa1 and HSD11B2, were differentially expressed between fast-, medium- and slow-growing P. vachelli. Sema7a, associated with hypoxia tolerance, was induced after hypoxia exposure and reoxygenation. Conclusions We mapped a set of suggestive and significant QTLs as well as candidate genes for 12 growth, 1 sex determination and 1 hypoxia tolerance related traits based on a high-density genetic linkage map by making use of SNP markers for P. fulvidraco. Our results have offered a valuable method about the much more efficient production of all-male, fast growth and hypoxia tolerance P. vachelli for the aquaculture industry.


2009 ◽  
Vol 43 (4) ◽  
pp. 345-350 ◽  
Author(s):  
Vidya JAYASANKAR ◽  
Safiah JASMANI ◽  
Takeshi NOMURA ◽  
Setsuo NOHARA ◽  
Do Thi Thanh HUONG ◽  
...  

2010 ◽  
Vol 41 (3) ◽  
pp. 286-294 ◽  
Author(s):  
Z.-Q. Du ◽  
D. C. Ciobanu ◽  
S. K. Onteru ◽  
D. Gorbach ◽  
A. J. Mileham ◽  
...  

2011 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Ferdinand Hukama Taqwa ◽  
Daniel Djokosetiyanto ◽  
Ridwan Affandi

<p>This research was conducted to determine natural food substitution time by artificial diet   after salinity acclimatization from 20 ppt until 2 ppt, which can increase survival and growth of (<em>Litopenaeus vannamei</em>) postlarvae during rearing period. Design experiment was completely randomized design with five treatments and three replications of natural food <em>Chironomus</em> sp.  (60% of crude protein) substitution time by artificial diet (40% of crude protein) at day: 1 (A), 7 (B), 14 (C), 21 (D) and full natural food without artificial diet (E) during 28 days rearing period. White shrimp postlarvae and rearing media in this experiment based from best result of earlier research that is PL<sub>25 </sub>from acclimatization in media 2 ppt with addition of potassium 25 ppm to freshwater media.  The densities of PL<sub>25</sub> white shrimp were 20 PLs/50 liters of 2 ppt media. The result of this experiment showed that the use of artificial diet as soon as after salinity acclimatization (PL<sub>25</sub>) gave best performance production compared to which only that was given natural food <em>Chironomus</em> sp. during experiment or with treatment by artificial diet substitution at day-7, day-14 or day-21, shown with the highest value of food consumption level, protein retention, energy retention, daily growth rate and food efficiency. Survival rate of PL<sub>54</sub> was above 80% and not significant different between treatment. That is supported by chemical-physical value of water quality still in range appropriate to survival rate of white shrimp post larvae during a rearing period. The result of this experiment indicated that requirement nutrient of PL<sub>25</sub> in low salinity did not fulfilled if only rely on natural food, so that require artificial diet with nutrition content to support growth and survival rate of white shrimp post larvae more maximal.</p> <p>Key words: salinity, natural food, artificial diet, Pacific white shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tujuan penelitian ini adalah untuk menentukan waktu penggantian pakan alami oleh pakan buatan yang tepat selama masa pemeliharaan postlarva udang vaname di media bersalinitas rendah setelah melalui masa aklimatisasi penurunan salinitas dari 20 ppt hingga 2 ppt, sehingga dapat meningkatkan pertumbuhan dan kelangsungan hidup. Rancangan percobaan berupa rancangan acak lengkap dengan perlakuan yang diterapkan berupa waktu penggantian pakan alami <em>Chironomus </em>sp. (kadar protein 62%) oleh pakan buatan (kadar protein 40%) pada hari ke-1 (A), ke-7 (B), ke-14 (C), ke-21 (D) dan pakan alami (E) selama masa pemeliharaan. Postlarva udang vaname dan media pemeliharaan yang dipergunakan selama percobaan mengacu pada hasil terbaik yang didapatkan dari penelitian pendahuluan yaitu berupa PL<sub>25 </sub>hasil aklimatisasi di media bersalinitas 2 ppt  dengan penambahan kalium 25 ppm ke media air tawar pengencer. Padat tebar sebanyak 20 ekor/50 liter/wadah. Hasil percobaan menunjukkan bahwa pemberian pakan buatan yang diberikan segera setelah masa aklimatisasi salinitas (pada awal pemeliharaan PL<sub>25</sub>) memberikan performa produksi budidaya terbaik bila dibandingkan dengan yang hanya diberi pakan alami selama masa pemeliharaan maupun waktu penggantian pakan alami oleh pakan buatan pada hari ke-7, ke-14 dan hari ke-21 yang ditunjukkan dengan tingkat konsumsi pakan, retensi protein, retensi energi, laju pertumbuhan harian dan efisiensi pakan yang tertinggi.  Kelangsungan hidup di akhir pemeliharaan (PL<sub>54</sub>)  di atas 80% dan tidak berbeda nyata antar perlakuan. Hal ini ditunjang oleh nilai fisika kimia air yang berada dalam kisaran yang layak selama masa pemeliharaan. Hasil percobaan ini menunjukkan bahwa kebutuhan nutrisi pada stadia PL<sub>25</sub> di media bersalinitas rendah tidak terpenuhi jika hanya mengandalkan pakan alami sehingga perlu ditunjang dari pakan buatan dengankandungan nutrisi yang dapat mendukung pertumbuhan dan kelangsungan hidup yang lebih maksimal.</p> <p>Kata kunci: salinitas, pakan alami, pakan buatan, udang vaname.</p>


Sign in / Sign up

Export Citation Format

Share Document