Distribution and localization of Streptococcus agalactiae in different tissues of artificially infected tilapia (Oreochromis niloticus)

Aquaculture ◽  
2022 ◽  
Vol 546 ◽  
pp. 737370
Author(s):  
Jianmeng Cao ◽  
Zhigang Liu ◽  
Defeng Zhang ◽  
Fuqiang Guo ◽  
Fengying Gao ◽  
...  
2019 ◽  
Vol 15 ◽  
pp. 100232 ◽  
Author(s):  
Mengmeng Yi ◽  
Miao Wang ◽  
Zhonghui Li ◽  
Zhigang Liu ◽  
Changbin Song ◽  
...  

Aquaculture ◽  
2016 ◽  
Vol 454 ◽  
pp. 237-242 ◽  
Author(s):  
Guilherme Campos Tavares ◽  
Frederico Augusto de Alcântara Costa ◽  
Raquel Ribeiro Dias Santos ◽  
Gustavo Morais Barony ◽  
Carlos Augusto Gomes Leal ◽  
...  

2022 ◽  
Vol 10 (1) ◽  
pp. 100
Author(s):  
Po-Tsang Lee ◽  
Yu-Sheng Wu ◽  
Chung-Chih Tseng ◽  
Jia-Yu Lu ◽  
Meng-Chou Lee

This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.


2018 ◽  
Vol 1 (1) ◽  
pp. 40
Author(s):  
Sri Wulandari ◽  
Rahmad Jumadi ◽  
Firma Fika Rahmawati

The main problem in the cultivation of tilapia is the attack of bacterial disease Streptococcosiscaused by Streptococcus agalactiae bacteria. Alternative measures to prevent the disease ofStreptocococis can be through the use of phytopharmaca materials. One of the ingredients ofphytopharmaca can be used is cinnamon plant. Cinnamon leaves contain several types of activesubstances such as tannins, eugenol, safrole, calcium oxalate, resin, saponins, tanners, andsinamaldehid. The objective of the study of effectiveness of leaf powder of cinnamon plant toleukocyte differential and phagocytic activity in tilapia (Oreochromis niloticus) infected byStreptococcus agalactiae is to know the influence and dosage of cinnamon leaf powder in feedtoLeukocyte Differentiation and Phagocytosis Activity Postcainfection Streptococcus agalactiaein tilapia (Oreochromis niloticus). This research used 3treatment 4 replication and control withdose K- = challenge test, K+ = without test challenge, A = dose 0,25%, B = dose 0,5%, C = 1%.The parameters observed are Leukocyte Differential and Phagocytosis Activity. Dosage 0.5%addition of leaf powder cinnamon plant on feed is the best dose. Giving of cinnamon leaf powderin feed influenced to increase of leukocyte difference especially on monocyte cell and neutrophilcell was significantly different (P>0,05) than K+ without addition of cinnamon leaf powderwhile phagocytic activity had an effect on Streptococcus agacatiae.


2018 ◽  
Vol 18 (4) ◽  
pp. 634 ◽  
Author(s):  
Amalia Putri Firdausi ◽  
Sukenda Sukenda ◽  
Sri Nuryati

The objective of this study was to evaluate the effectiveness of vaccination on tilapia seedlings resulted from the vaccinated parent by hyperosmotic infiltration method at four different salinity to prevent Streptococcus agalactiae bacterial infection. A total of 100 seeds aged 20 days from the vaccinated mother were immersed in four different salinity: 0 ppt (control), 10 ppt, 20 ppt, and 30 ppt for five minutes, then removed and transferred into vaccine-containing containers for 30 minutes. Further maintenance was performed in freshwater aquariums and at days 10, 20, and 30 post vaccination, the immune system performance: Relative Percent Survival (RPS) after the challenge test, specific antibody level, and lysozyme was observed. The study was conducted using a complete randomized design with three replications. The results showed that the 10 ppt salinity treatment gave the best results compared to the others and control. The final RPS (10 ppt) value was 84.72%, 66.49%, and 47.06%, on the 10th, 20th, and 30thh days of vaccination, respectively. Specific antibody levels (10 ppt) were 0.077, 0.078, and 0.077 and lysozyme 0.092, 0.084, and 0.032 at days 10, 20, and 30 post vaccination, respectively. The value of RPS and specific antibody level at 10 ppt salinity was significantly different (P <0.05) compared to the other treatments, while the lysozyme treatment activity of 10 ppt was not significantly different compared to the 20 ppt treatment. Vaccination using 10 ppt saline hyperosmotic infiltration can improve the performance of the immune system by improving maternal immune protection against Streptococcus agalactiae infection.


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