Molecular identification and pathogenicity study of virulent Vibrio cholerae non O1/O139 serotype associated with mortality of farmed Labeo rohita (Hamilton, 1822), in India

Aquaculture ◽  
2022 ◽  
Vol 547 ◽  
pp. 737529
Author(s):  
Manoharmayum Shaya Devi ◽  
Prasenjit Paria ◽  
Vikash Kumar ◽  
Pranaya Kumar Parida ◽  
Praveen Maurye ◽  
...  
1998 ◽  
Vol 93 (5) ◽  
pp. 601-607 ◽  
Author(s):  
MAS Baptista ◽  
JRC Andrade ◽  
ACP Vicente ◽  
CA Salles ◽  
A Coelho

2001 ◽  
Vol 127 (01) ◽  
Author(s):  
E. FOLGOSA ◽  
S. MASTRANDREA ◽  
P. CAPPUCCINELLI ◽  
S. UZZAU ◽  
P. RAPPELLI ◽  
...  

Author(s):  
S. E. Miller

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.


1970 ◽  
Vol 24 (1) ◽  
pp. 38-41
Author(s):  
Taslima Taher Lina ◽  
Mohammad Ilias

The in vivo production of soluble inorganic pyrophosphatases (PPases) was investigated in two strains, namely, Vibrio cholerae EM 004 (environmental strain) and Vibrio cholerae O1 757 (ATCC strain). V. cholerae is known to contain both family I and family II PPase coding sequences. The production of family I and family II PPases were determined by measuring the enzyme activity in cell extracts. The effects of pH, temperature, salinity of the growth medium on the production of soluble PPases were studied. In case of family I PPase, V. cholerae EM 004 gave the highest specific activity at pH 9.0, with 2% NaCl + 0.011% NaF and at 37°C. The strain V. cholerae O1 757 gave the highest specific activity at pH 9.0, with media containing 0% NaCl and at 37°C. On the other hand, under all the conditions family II PPase did not give any significant specific activity, suggesting that the family II PPase was not produced in vivo in either strains of V. cholerae under different experimental conditions. Keywords: Vibrio cholerae, Pyrophosphatases (PPases), Specific activityDOI: http://dx.doi.org/10.3329/bjm.v24i1.1235 Bangladesh J Microbiol, Volume 24, Number 1, June 2007, pp 38-41


2019 ◽  
Vol 45 (5) ◽  
pp. 525-532
Author(s):  
Ting Li ◽  
Hao Wu ◽  
Caiwen Wu ◽  
Guang Yang ◽  
Bingyao Chen

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