Effects of sub-lethal levels of dichlorodiphenyltrichloroethane and dichlorodiphenyldichloroethylene on in vitro steroid biosynthesis by ovarian follicles or steroid metabolism by embryos of rainbow trout (Oncorhynchus mykiss)

2005 ◽  
Vol 73 (3) ◽  
pp. 288-298
Author(s):  
Rakpong Petkam ◽  
Rick Renaud ◽  
Lucy Lin ◽  
Herman Boermans ◽  
John Leatherland
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Ovarian Follicles ◽  
Steroid Metabolism ◽  
Steroid Biosynthesis ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals The in vitro metabolism of cortisol by ovarian follicles of rainbow trout (Oncorhynchus mykiss): comparison with ovulated oocytes and pre-hatch embryos

Reproduction ◽  
2012 ◽  
Vol 144 (6) ◽  
pp. 713-722 ◽  
Author(s):  
Mao Li ◽  
Heather L Christie ◽  
John F Leatherland
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Ovarian Follicles ◽  
Tissue Samples ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Early Embryos ◽  
Vitellogenic Stage ◽  
Gst Activity

Mid-vitellogenic stage rainbow trout (Oncorhynchus mykiss) ovarian follicles (both intact and yolk free (YF)), ovulated oocytes and embryos were co-incubated with [2,4,6,7-3H]cortisol for 18 h to determine the degree and nature of the metabolism and biotransformation of the glucocorticoid. There was evidence of the conversion of cortisol to the less biologically potent glucocorticoid, cortisone, and the formation of glucocorticoid sulphates (both cortisol and cortisone) for all cell and tissue samples, suggesting the presence of 11β-hydroxysteroid dehydrogenase (11β-HSD) and glucocorticoid sulphotransferase (GST) activity at all stages; however, GST activity was particularly marked in both intact and YF ovarian follicles, suggesting an important role of follicles in limiting the exposure of oocyte to maternal cortisol. As there was no evidence of 11β-HSD or GST activity in ovarian fluid, the findings affirm that ovarian follicles (probably the thecal and granulosa cells) provide a barrier against the transfer of cortisol to the oocytes by forming sulphated steroids, whereas ovulated oocytes and early embryos have a more limited capacity to either metabolize or conjugate cortisol and are therefore more vulnerable at the post-ovulatory and early embryonic stages to increases in exposure to the glucocorticoid.

Estrogenic activity of multicyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss) in vitro assays

2019 ◽  
Vol 207 ◽  
pp. 43-51 ◽  
Author(s):  
Richard C. Kolanczyk ◽  
Jeffrey S. Denny ◽  
Barbara R. Sheedy ◽  
Patricia K. Schmieder ◽  
Mark A. Tapper
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Aromatic Hydrocarbons ◽  
Estrogenic Activity ◽  
In Vitro Assays ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals Gill amoebae from freshwater rainbow trout ( Oncorhynchus mykiss ): In vitro evaluation of antiparasitic compounds against Vannella sp.

2020 ◽  
Vol 43 (6) ◽  
pp. 665-672
Author(s):  
Hannah Malene Jensen ◽  
Asma Mohammad Karami ◽  
Heidi Mathiessen ◽  
Azmi Al‐Jubury ◽  
Per Walter Kania ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
In Vitro Evaluation ◽  
Rainbow Trout Oncorhynchus Mykiss
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