Carbonic anhydrase amyloid fibrils composed of laterally associated protofilaments show reduced cytotoxicity

Author(s):  
Natalya Ryabova ◽  
Liliia Fakhranurova ◽  
Vitaly Balobanov ◽  
Victor Marchenkov ◽  
Anatoly Glukhov ◽  
...  
2008 ◽  
Vol 1784 (6) ◽  
pp. 930-935 ◽  
Author(s):  
Anshul Rana ◽  
Teemish Praveen Gupta ◽  
Saurabh Bansal ◽  
Bishwajit Kundu

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1608
Author(s):  
Victor Marchenkov ◽  
Natalya Ryabova ◽  
Vitaly Balobanov ◽  
Anatoly Glukhov ◽  
Nelly Ilyina ◽  
...  

The development of many severe human diseases is associated with the formation of amyloid fibrils. Most of the available information on the process of amyloid formation has been obtained from studies of small proteins and peptides, wherein the features of complex proteins’ aggregation remain insufficiently investigated. Our work aimed to research the amyloid aggregation of a large model protein, bovine carbonic anhydrase B (BCAB). It has previously been demonstrated that, when exposed to an acidic pH and elevated temperature, this protein forms amyloid fibrils. Here, we show that, under these conditions and before amyloid formation, BCAB undergoes fragmentation by acid hydrolysis to give free individual peptides and associated peptides. Fragments in associates contain a pronounced secondary structure and act as the main precursor of amyloid fibrils, wherein free peptides adopt mostly unstructured conformation and form predominantly irregular globular aggregates. Reduced acidity decreases the extent of acid hydrolysis, causing BCAB to form amorphous aggregates lacking the thioflavin T binding β-structure. The presented results provide new information on BCAB amyloid formation and show the importance of protein integrity control when working even in mildly acidic conditions.


2020 ◽  
Vol 524 (2) ◽  
pp. 453-458
Author(s):  
Liliia Fakhranurova ◽  
Vitaly Balobanov ◽  
Natalya Ryabova ◽  
Anatoly Glukhov ◽  
Nelly Ilyina ◽  
...  

Author(s):  
D.F. Clapin ◽  
V.J.A. Montpetit

Alzheimer's disease is characterized by the accumulation of abnormal filamentous proteins. The most important of these are amyloid fibrils and paired helical filaments (PHF). PHF are located intraneuronally forming bundles called neurofibrillary tangles. The designation of these structures as "tangles" is appropriate at the light microscopic level. However, localized domains within individual tangles appear to demonstrate a regular spacing which may indicate a liquid crystalline phase. The purpose of this paper is to present a statistical geometric analysis of PHF packing.


Author(s):  
Judith A. Murphy ◽  
Anthony Paparo ◽  
Richard Sparks

Fingernail clams (Muscu1ium transversum) are dominant bottom-dwelling animals in some waters of the midwest U.S. These organisms are key links in food chains leading from nutrients in water and mud to fish and ducks which are utilized by man. In the mid-1950’s, fingernail clams disappeared from a 100-mile section of the Illinois R., a tributary of the Mississippi R. Some factor(s) in the river and/or sediment currently prevent clams from recolonizing areas where they were formerly abundant. Recently, clams developed shell deformities and died without reproducing. The greatest mortality and highest incidence of shell deformities appeared in test chambers containing the highest proportion of river water to well water. The molluscan shell consists of CaCO3, and the tissue concerned in its secretion is the mantle. The source of the carbonate is probably from metabolic CO2 and the maintenance of ionized Ca concentration in the mantle is controlled by carbonic anhydrase. The Ca is stored in extracellular concentric spherical granules(0.6-5.5μm) which represent a large amount of inertCa in the mantle. The purpose of this investigation was to examine the role of raw river water and well water on shell formation in the fingernail clam.


1955 ◽  
Vol 29 (2) ◽  
pp. 262-279 ◽  
Author(s):  
David A. Dreiling ◽  
Henry D. Janowitz ◽  
Mark Halpern

2004 ◽  
Vol 171 (4S) ◽  
pp. 206-207 ◽  
Author(s):  
Hideki Mukouyama ◽  
Masahiro Yao ◽  
David B. Seligson ◽  
John S. Lam ◽  
Yoji Nagashima ◽  
...  

2004 ◽  
Vol 171 (4S) ◽  
pp. 296-296
Author(s):  
Michael Straub ◽  
Joséphine Befolo-Elo ◽  
Richard E Hautmann ◽  
Edgar Braendle

Skull Base ◽  
2007 ◽  
Vol 17 (S 2) ◽  
Author(s):  
Heon Yoo ◽  
Gilson Baia ◽  
Justin Smith ◽  
Michael McDermott ◽  
Andrew Bollen ◽  
...  

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