Effects of short-term fasting on the rhythmic expression of core circadian clock and functional genes in skeletal muscle of goldfish (Carassius auratus)

2018 ◽  
Vol 226 ◽  
pp. 91-98 ◽  
Author(s):  
Lingsheng Bao ◽  
Jianhua Wang ◽  
Shiyu Bin ◽  
Yulong Li ◽  
Wuying Chu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Circadian Clock ◽  
Carassius Auratus ◽  
Functional Genes ◽  
Short Term ◽  
Rhythmic Expression ◽  
Goldfish Carassius Auratus

Effects of short-term starvation on the rhythmic expression of microRNAs in skeletal muscle of goldfish (Carassius auratus )

2017 ◽  
Vol 49 (2) ◽  
pp. 726-737 ◽  
Author(s):  
Ping Wu ◽  
Jun Shi ◽  
Chengyong Yang ◽  
Fangliang Zhang ◽  
Yulong Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Carassius Auratus ◽  
Short Term ◽  
Rhythmic Expression ◽  
Goldfish Carassius Auratus

scholarly journals Impact of Short-Term Fasting on The Rhythmic Expression of the Core Circadian Clock and Clock-Controlled Genes in Skeletal Muscle of Crucian Carp (Carassius auratus)

Genes ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 526 ◽  
Author(s):  
Ping Wu ◽  
Lingsheng Bao ◽  
Ruiyong Zhang ◽  
Yulong Li ◽  
Li Liu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Circadian Rhythms ◽  
Crucian Carp ◽  
Clock Genes ◽  
Functional Genes ◽  
Short Term ◽  
Peripheral Tissues ◽  
Circadian Genes ◽  
Rhythmic Expression ◽  
The Core

The peripheral tissue pacemaker is responsive to light and other zeitgebers, especially food availability. Generally, the pacemaker can be reset and entrained independently of the central circadian structures. Studies involving clock-gene expressional patterns in fish peripheral tissues have attracted considerable attention. However, the rhythmic expression of clock genes in skeletal muscle has only scarcely been investigated. The present study was designed to investigate the core clock and functional gene expression rhythms in crucian carp. Meanwhile, the synchronized effect of food restrictions (short-term fasting) on these rhythms in skeletal muscle was carefully examined. In fed crucian carp, three core clock genes (Clock, Bmal1a, and Per1) and five functional genes (Epo, Fas, IGF1R2, Jnk1, and MyoG) showed circadian rhythms. By comparison, four core clock genes (Clock, Bmal1a, Cry3, and Per2) and six functional genes (Epo, GH, IGF2, Mstn, Pnp5a, and Ucp1) showed circadian rhythms in crucian carp muscle after 7-day fasting. In addition, three core clock genes (Clock, Per1, and Per3) and six functional genes (Ampk1a, Lpl, MyoG, Pnp5a, PPARα, and Ucp1) showed circadian rhythms in crucian carp muscle after 15-day fasting. However, all gene rhythmic expression patterns differed from each other. Furthermore, it was found that the circadian genes could be altered by feed deprivation in crucian carp muscle through the rhythms correlation analysis of the circadian genes and functional genes. Hence, food-anticipatory activity of fish could be adjusted through the food delivery restriction under a light–dark cycle. These results provide a potential application in promoting fish growth by adjusting feeding conditions and nutritional state.

Brain lesions and short-term endocrine effects of monosodium L-glutamate in goldfish, Carassius auratus

1980 ◽  
Vol 212 (3) ◽  
Author(s):  
RichardE. Peter ◽  
Olivier Kah ◽  
ChristineR. Paulencu ◽  
Harry Cook ◽  
AnnL. Kyle
Keyword(s):  
Carassius Auratus ◽  
Brain Lesions ◽  
Endocrine Effects ◽  
Short Term ◽  
Goldfish Carassius Auratus

Anaerobic metabolism in goldfish (Carassius auratus)

1977 ◽  
Vol 55 (8) ◽  
pp. 1304-1311 ◽  
Author(s):  
R. M. Walker ◽  
P. H. Johansen
Keyword(s):  
Carassius Auratus ◽  
Glycogen Phosphorylase ◽  
Anaerobic Metabolism ◽  
Liver Glycogen ◽  
Energy Yield ◽  
Low Oxygen ◽  
Short Term ◽  
Liver Size ◽  
Goldfish Carassius Auratus ◽  
Glycogen Stores

At 20 °C goldfish survive anaerobic conditions for only a few hours while at 4 °C survival is extended to several days. During the course of low-temperature anaerobiosis there was a rise in blood glucose and lactate, a decline in liver glycogen concentration, and an increase in liver water content, while liver size remained constant.The better cold anaerobic survival of winter and hypophysectomized goldfish compared with spring and sham-operated animals was correlated with greater glycogen stores in the livers of the former. It is concluded that liver glycogen is a necessary energy source during cold anaerobiosis, and it is suggested that the resulting hyperglycemia may represent a mechanism to increase glycolytic energy yield. Cold anaerobiosis also resulted in elevated liver glucose-6-phosphatase (EC 3.1.3.9) activity, suggesting an increase in glycogenolysis, but no change in glycogen phosphorylase (EC 2.4.1.1) activity. While cold anaerobic survival is short term it is possible that liver glycogen may sustain goldfish for longer periods at low oxygen levels through a mixed aerobic–anaerobic metabolism.

Feeding Time Synchronizes Clock Gene Rhythmic Expression in Brain and Liver of Goldfish (Carassius auratus)

2011 ◽  
Vol 26 (1) ◽  
pp. 24-33 ◽  
Author(s):  
Andrea Feliciano ◽  
Yurena Vivas ◽  
Nuria de Pedro ◽  
María J. Delgado ◽  
Elena Velarde ◽  
...  
Keyword(s):  
Carassius Auratus ◽  
Clock Gene ◽  
Feeding Time ◽  
Rhythmic Expression ◽  
Goldfish Carassius Auratus

Short-Term Serum Prolactin Concentrations in Goldfish (Carassius auratus) Subjected to Serial Sampling and Restraint

1976 ◽  
Vol 33 (1) ◽  
pp. 183-186 ◽  
Author(s):  
Richard E. Spieler ◽  
Albert H. Meier
Keyword(s):  
Carassius Auratus ◽  
Marked Decrease ◽  
Circadian Variation ◽  
Serum Prolactin ◽  
Short Term ◽  
Goldfish Carassius Auratus ◽  
Serial Sampling ◽  
The Times ◽  
Initial Capture

Serum prolactin concentrations in serially sampled goldfish (Carassius auratus) did not vary significantly between 30 s and 3 min after initial capture. A marked decrease in prolactin concentration was noted 9–17 min after initial capture followed by a recovery at 30–48 min. Although there was a circadian variation in prolactin concentration, the pattern of response to capture and sampling did not vary significantly among the times tested.

scholarly journals Targeted Disruption of the mPer3 Gene: Subtle Effects on Circadian Clock Function

2000 ◽  
Vol 20 (17) ◽  
pp. 6269-6275 ◽  
Author(s):  
Lauren P. Shearman ◽  
Xiaowei Jin ◽  
Choogon Lee ◽  
Steven M. Reppert ◽  
David R. Weaver
Keyword(s):  
Skeletal Muscle ◽  
Circadian Clock ◽  
Wild Type ◽  
Targeted Disruption ◽  
Rhythmic Expression ◽  
Helix Loop Helix ◽  
Mutant Transcript ◽  
Circadian Timing System ◽  
Positive Regulators ◽  
A Cell

ABSTRACT Neurons in the mammalian suprachiasmatic nucleus (SCN) contain a cell-autonomous circadian clock that is based on a transcriptional-translational feedback loop. The basic helix-loop-helix–PAS proteins CLOCK and BMAL1 are positive regulators and drive the expression of the negative regulators CRY1 and CRY2, as well as PER1, PER2, and PER3. To assess the role of mouse PER3 (mPER3) in the circadian timing system, we generated mice with a targeted disruption of the mPer3 gene. Western blot analysis confirmed the absence of mPER3-immunoreactive proteins in mice homozygous for the targeted allele. mPer1,mPer2, mCry1, and Bmal1 RNA rhythms in the SCN did not differ between mPER3-deficient and wild-type mice. Rhythmic expression of mPer1 and mPer2 RNAs in skeletal muscle also did not differ between mPER3-deficient and wild-type mice. mPer3 transcripts were rhythmically expressed in the SCN and skeletal muscle of mice homozygous for the targeted allele, but the level of expression of the mutant transcript was lower than that in wild-type controls. Locomotor activity rhythms in mPER3-deficient mice were grossly normal, but the circadian cycle length was significantly (0.5 h) shorter than that in controls. The results demonstrate that mPer3 is not necessary for circadian rhythms in mice.

Sign in / Sign up

Export Citation Format

Share Document