Analysis of endocrine disrupters and related compounds in sediments and sewage sludge using on-line turbulent flow chromatography–liquid chromatography–tandem mass spectrometry

2014 ◽  
Vol 1352 ◽  
pp. 29-37 ◽  
Author(s):  
Marina Gorga ◽  
Sara Insa ◽  
Mira Petrovic ◽  
Damià Barceló
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Sewage Sludge ◽  
Tandem Mass Spectrometry ◽  
Turbulent Flow ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
On Line ◽  
Related Compounds

scholarly journals A Comprehensive Analysis Using Colorimetry, Liquid Chromatography-Tandem Mass Spectrometry and Bioassays for the Assessment of Indole Related Compounds Produced by Endophytes of Selected Wheat Cultivars

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1394
Author(s):  
Agnieszka Kuźniar ◽  
Kinga Włodarczyk ◽  
Ilona Sadok ◽  
Magdalena Staniszewska ◽  
Małgorzata Woźniak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Endophytic Bacteria ◽  
Culture Medium ◽  
Tandem Mass ◽  
Wheat Cultivars ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Related Compounds

Liquid chromatography–tandem mass spectrometry (LC–MS/MS), colorimetry, and bioassays were employed for the evaluation of the ability of endophytic bacterial strains to synthesize indole-related compounds (IRCs) and in particular indole-3-acetic acid (IAA). A total of 54 endophytic strains belonging to seven bacterial genera isolated from tissues of common and spelt wheat cultivars were studied. The endophytic bacteria isolated from different tissues of the tested wheat types were capable of IRCs production, including IAA, which constituted from 1.75% to 52.68% of all IRCs, in in vitro conditions via the tryptophan dependent pathway. The selected post-culture medium was also examined using a plant bioassay. Substantial growth of wheat coleoptile segments treated with the bacterial post-culture medium was observed in several cases. Our data suggest that the studied endophytic bacteria produce auxin-type compounds to support plant development. Summarizing, our approach to use three complementary methods for estimation of IRCs in different endophytic strains provides a comprehensive picture of their effect on wheat growth.

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