Evaluation of guanidine-based multimodal anion exchangers for protein selectivity and orthogonality

2021 ◽  
pp. 462398
Author(s):  
Sushmita Koley ◽  
Scott H. Altern ◽  
Mayank Vats ◽  
Xuan Han ◽  
Dongyoun Jang ◽  
...  
Keyword(s):  
Anion Exchangers

Cr(VI) RETENTION CAPACITY AND SELECTIVITY OF SOME STRONG AND WEAK BASE ANION EXCHANGERS

2004 ◽  
Vol 3 (3) ◽  
pp. 221-230 ◽  
Author(s):  
Ion Untea ◽  
Cristina Orbeci ◽  
Elena Tudorache ◽  
Violeta Neagu
Keyword(s):  
Weak Base ◽  
Anion Exchangers ◽  
Retention Capacity

scholarly journals Purification of rabbit polyclonal immunoglobulin G using anion exchangers

2011 ◽  
Vol 46 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Rattana Wongchuphan ◽  
Beng Ti Tey ◽  
Wen Siang Tan ◽  
Senthil Kumar Subramanian ◽  
Farah Saleena Taip ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Anion Exchangers ◽  
Polyclonal Immunoglobulin

scholarly journals AE anion exchangers in atrial tumor cells

2001 ◽  
Vol 280 (3) ◽  
pp. H937-H945 ◽  
Author(s):  
Panos Papageorgiou ◽  
Boris E. Shmukler ◽  
Alan K. Stuart-Tilley ◽  
Lianwei Jiang ◽  
Seth L. Alper
Keyword(s):  
Tumor Cell Line ◽  
Disulfonic Acid ◽  
Cell Type ◽  
Intracellular Acidification ◽  
Ph Homeostasis ◽  
Rt Pcr ◽  
Anion Exchangers ◽  
Atrial Tumor ◽  
Atrial Cell ◽  
Exchange Activity

Intracellular pH homeostasis and intracellular Cl−concentration in cardiac myocytes are regulated by anion exchange mechanisms. In physiological extracellular Cl−concentrations, Cl−/HCO[Formula: see text] exchange promotes intracellular acidification and Cl−loading sensitive to inhibition by stilbene disulfonates. We investigated the expression of AE anion exchangers in the AT-1 mouse atrial tumor cell line. Cultured AT-1 cells exhibited a substantial basal Na+-independent Cl−/HCO[Formula: see text] (but not Cl−/OH−) exchange activity that was inhibited by DIDS but not by dibenzamidostilbene disulfonic acid (DBDS). AT-1 cell Cl−/HCO[Formula: see text] activity was stimulated two- to threefold by extracellular ATP and ANG II. AE mRNAs detected by RT-PCR in AT-1 cells included brain AE3 (bAE3), cardiac AE3 (cAE3), AE2a, AE2b, AE2c1, AE2c2, and erythroid AE1 (eAE1), but not kidney AE1 (kAE1). Cultured AT-1 cells expressed AE2, cAE3, and bAE3 polypeptides, which were detected by immunoblot and immunocytochemistry. An AE1-like epitope was detected by immunocytochemistry but not by immunoblot. Both bAE3 and cAE3 were present in intact AT-1 tumors. Cultured AT-1 cells provide a useful system for the study of mediators and regulators of Cl−/HCO[Formula: see text] exchange activity in an atrial cell type.

Comparative study of strong anion exchangers: Structure-related chromatographic performances

2009 ◽  
Vol 877 (24) ◽  
pp. 2443-2450 ◽  
Author(s):  
Jerome Pezzini ◽  
Charlotte Cabanne ◽  
Xavier Santarelli
Keyword(s):  
Comparative Study ◽  
Anion Exchangers

Regulation of ion transport by pH and [HCO3−] in isolated gills of the crab Neohelice (Chasmagnathus) granulata

2008 ◽  
Vol 294 (3) ◽  
pp. R1033-R1043 ◽  
Author(s):  
Martin Tresguerres ◽  
Scott K. Parks ◽  
Sebastian E. Sabatini ◽  
Greg G. Goss ◽  
Carlos M. Luquet
Keyword(s):  
Carbonic Anhydrase ◽  
Ion Transport ◽  
Acid Secretion ◽  
Cellular Response ◽  
Low Ph ◽  
Potential Difference ◽  
Anion Exchangers ◽  
Transepithelial Potential Difference ◽  
Chasmagnathus Granulata ◽  
First Time

Posterior isolated gills of Neohelice ( Chasmagnathus) granulatus were symmetrically perfused with hemolymph-like saline of varying [HCO3−] and pH. Elevating [HCO3−] in the saline from 2.5 to 12.5 mmol/l (pH 7.75 in both cases) induced a significant increase in the transepithelial potential difference ( Vte), a measure of ion transport. The elevation in [HCO3−] also induced a switch from acid secretion (−43.7 ± 22.5 μequiv·kg−1·h−1) in controls to base secretion (84.7 ± 14.4 μequiv·kg−1·h−1). The HCO3−-induced Vte increase was inhibited by basolateral acetazolamide (200 μmol/l), amiloride (1 mmol/l), and ouabain (5 mmol/l) but not by bafilomycin (100 nmol/l). The Vte response to HCO3− did not take place in Cl−-free conditions; however, it was unaffected by apical SITS (2 mmol/l) or DIDS (1 mmol/l). A decrease in pH from 7.75 to 7.45 pH units in the perfusate also induced a significant increase in Vte, which was matched by a net increase in acid secretion of 67.8 ± 18.4 μequiv kg−1 h−1. This stimulation was sensitive to basolateral acetazolamide, bafilomycin, DIDS, and Na+-free conditions, but it still took place in Cl−-free saline. Therefore, the cellular response to low pH is different from the HCO3−-stimulated response. We also report V-H+-ATPase- and Na+-K+-ATPase-like immunoreactivity in gill sections for the first time in this crab. Our results suggest that carbonic anhydrase (CA), basolateral Na+/H+ exchangers and Na+-K+-ATPase and apical anion exchangers participate in the HCO3−-stimulated response, while CA, apical V-H+-ATPase and basolateral HCO3−-dependent cotransporters mediate the response to low pH.

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