Rifampin resistance mutations in the rpoB gene of Enterococcus faecalis impact host macrophage cytokine production

Cytokine ◽  
2022 ◽  
Vol 151 ◽  
pp. 155788
Author(s):  
Darya V. Urusova ◽  
Joseph A. Merriman ◽  
Ananya Gupta ◽  
Liang Chen ◽  
Barun Mathema ◽  
...  
2002 ◽  
Vol 184 (17) ◽  
pp. 4936-4940 ◽  
Author(s):  
Wayne L. Nicholson ◽  
Heather Maughan

ABSTRACT Mutations causing rifampin resistance in vegetative cells of Bacillus subtilis 168 have thus far been mapped to a rather restricted set of alterations at either Q469 or H482 within cluster I of the rpoB gene encoding the β subunit of RNA polymerase. In this study, we demonstrated that spores of B. subtilis 168 exhibit a spectrum of spontaneous rifampin resistance mutations distinct from that of vegetative cells. In addition to the rpoB mutations Q469K, Q469R, and H482Y previously characterized in vegetative cells, we isolated a new mutation of rpoB, H482R, from vegetative cells. Additional new rifampin resistance mutations arising from spores were detected at A478N and most frequently at S487L. The S487L change is the predominant change found in rpoB mutations sequenced from rifampin-resistant clinical isolates of Mycobacterium tuberculosis. The observations are discussed in terms of the underlying differences of the DNA environment within dormant cells and vegetatively growing cells.


2017 ◽  
Vol 5 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Hemanta Kumari Chaudhary ◽  
Mitesh Shrestha ◽  
Prakash Chaudhary ◽  
Bal Hari Poudel

Multidrug-resistant tuberculosis (MDR-TB) has become a serious worldwide threat including in Nepal. MDR-TB refers to the pathological condition whereby Mycobacterium tuberculosis becomes resistant to the first line of drug treatment i.e. rifampin and isoniazid. Resistance to rifampin (RIF) is mainly caused by the mutations in the rpoB gene which codes for the β-subunit of RNA polymerase. In this study, Amplification Refractory Mutation System – Polymerase Chain Reaction (ARMS – PCR) technique has been used to detect mutations in the rpoB gene of Mycobacterium tuberculosis. Total DNA samples of 34 phenotypic MDR-TB were subjected to ARMS – PCR using three different codon specific primers (516, 526 and 531). These three codons occupy large portion of total mutation responsible for rifampin resistance. Out of the total DNA samples, all were bearing mutation in at least one of the three codons mentioned. Of those bearing mutation, the highest number had mutation in codon 531 (97.05 %) followed by codon 516 (17.64 %) and finally in codon 526 (11.76%) respectively. Hence, ARMS – PCR may be used as an alternative diagnostic technique for detection of rifampin resistance in Mycobacterium tuberculosis strains, especially for a developing country like Nepal.Int. J. Appl. Sci. Biotechnol. Vol 5(1): 81-85


2011 ◽  
Vol 187 (6) ◽  
pp. 3072-3086 ◽  
Author(s):  
Katja Seider ◽  
Sascha Brunke ◽  
Lydia Schild ◽  
Nadja Jablonowski ◽  
Duncan Wilson ◽  
...  

1997 ◽  
Vol 41 (9) ◽  
pp. 2059-2060 ◽  
Author(s):  
C Riesenfeld ◽  
M Everett ◽  
L J Piddock ◽  
B G Hall

Mutation to ciprofloxacin resistance continually occurred in nondividing Escherichia coli cells during a 7-day exposure to ciprofloxacin in agar, while no accumulation of rifampin resistance mutations was detected in those cells. We propose that the resistance mutations result from adaptive mutations, which preferentially produce phenotypes that promote growth in nondividing cells.


ChemBioChem ◽  
2018 ◽  
Vol 19 (14) ◽  
pp. 1476-1481 ◽  
Author(s):  
Tamim Mosaiab ◽  
Sandra Boiteux ◽  
Abu Hasanat Md Zulfiker ◽  
Ming Q. Wei ◽  
Milton J. Kiefel ◽  
...  

Author(s):  
Silvia Franchi ◽  
Mara Castelli ◽  
Sarah Moretti ◽  
Alberto Panerai ◽  
Paola Sacerdote

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