Gene and oligonucleotide delivery via micro- and nanobubbles by ultrasound exposure

The purpose. In the study we investigated the impact of the partial blood flow shutdown on structural changes in the rabbit vena cava posterior wall after exposure to high-intensity focused ultrasound (HIFU). Methods. Ultrasound Exposure: frequency of 1.65 MHz, the ultrasound intensity in the focus of 13.6 kW/cm, the area of the focal spot 1 mm, continuous ultrasound, exposure for 3 seconds. Results. Immediately after HIFU exposure all layers of the vein wall showed characteristic signs of thermal damage. A week after exposure structural changes in the intima, media and adventitia was minimal in the part of vessel with preserved blood flow, and after 4 weeks the changes were not revealed. A week after HIFU exposure partial endothelium destruction, destruction of myocytes, disorganization and consolidation of collagen fibers of the adventitia were observed in an isolated segment of the vessel, and in 4 weeks endothelium restored and signs of damage in media and adventitia persisted, but were less obvious than in a week after exposure. Conclusion. The shutdown of blood flow after exposure to HIFU promotes persistent changes in the vein wall. Vein compression appears to be necessary for the obliteration of the vessel, when using HIFU-technology.

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On the safety of ultrasound exposure

Choonpa Igaku ◽

10.3179/jjmu.35.623 ◽

2008 ◽

Vol 35 (6) ◽

pp. 623-629 ◽

Cited By ~ 3

Author(s):

Nobuki KUDO ◽

Katsuyuki YAMAMOTO

Keyword(s):

Ultrasound Exposure

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Characterize ultrasound exposure used for experimentsPart 1. How to report ultrasound exposure conditions

Choonpa Igaku ◽

10.3179/jjmu.jjmu.t.1 ◽

2016 ◽

Vol 43 (4) ◽

pp. 573-575

Author(s):

Miwa NAITO

Keyword(s):

Ultrasound Exposure ◽

Exposure Conditions

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Researches on the Synthesis and Biological Activity of 1,2,4-tiadizoles-3,5-disubstituted using Ditiazolium Salts as Precursors

Revista de Chimie ◽

10.37358/rc.08.1.1716 ◽

2008 ◽

Vol 59 (1) ◽

pp. 101-105

Author(s):

Irina Zarafu ◽

Lucia Veronica Ivan ◽

Iuliana Harasim

Keyword(s):

Biological Activity ◽

Comparative Study ◽

Anticancer Activity ◽

Reaction Process ◽

Physical Analysis ◽

Ultrasound Exposure ◽

Medium Activity ◽

Antibacterial And Anticancer Activity

3,5-disubstituted-1,2,4-tiadiazoles with substituted-styril and heterocycle-vinyl were obtained by extending the method which implies the use of 3,5-disubstituted-1,2,4-ditiazolium salts as precursors [1-4]. A comparative study of the reaction process in the case of perchlorates, diacide phosphates, tribromides and 3,5-distyrile-dithiazolium triiodides, taken as etalon, was perfomed. Good yields were obtained when using perchlorates, phosphates and triiodides. The reaction was made by heating the reaction mixture and by ultrasound exposure. The structure of the compounds was confirmed by chemical and physical analysis and the data obtained were identical to those of 3,5-disubstituted-1,2,4-tiadiazoles obtained by another methods [5,6]. The biological (antibacterial and anticancer) activity of the synthesized compounds was tested and the results indicated a medium activity.

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