Oleic acid increases mitochondrial reactive oxygen species production and decreases endothelial nitric oxide synthase activity in cultured endothelial cells

2015 ◽  
Vol 751 ◽  
pp. 67-72 ◽  
Author(s):  
Hendrik Gremmels ◽  
Lonneke M. Bevers ◽  
Joost O. Fledderus ◽  
Branko Braam ◽  
Anton Jan van Zonneveld ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Endothelial Nitric Oxide Synthase ◽  
Reactive Oxygen Species Production ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species ◽  
Species Production ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide ◽  
Nitric Oxide Synthase Activity

Danshensu Attenuates Hypoxia-Induced Reactive Oxygen Species Production and Inducible Nitric Oxide Synthase Expression in RAW 264.7 Cells

2019 ◽  
Vol 18 (1) ◽  
pp. 89-95
Author(s):  
Lin Chih-Hung ◽  
Lan Chou-Chin ◽  
Chiu Valeria ◽  
Hsieh Po-Chun ◽  
Kuo Chan-Yen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Oxygen Species ◽  
Species Production ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Danshensu, isolated from Salvia miltiorrhiza (Danshen), is known to have anti-inflammatory properties. Therefore danshen is extensively used in many nutraceutical formulations. Reactive oxygen species are essential for the development of hypoxia-induced inflammation. Generation of reactive oxygen species by infiltrating macrophages is common in various diseases such as cardiovascular disease, neurodegenerative disease, tumor, and aging. To explore the mechanism underlying the attenuation of inflammation, we used RAW 264.7 cells as a model and hypoxia as an inducer of inflammation. The results showed the protective mechanism of danshensu on reactive oxygen species production, hypoxia-inducible factor 1-alpha expression, c-Jun N-terminal kinase phosphorylation, nuclear translocation of nuclear factor kappa B, and inducible nitric oxide synthase expression following hypoxia in RAW 264.7 cells.

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