Background:
This study reports an easy method of a veterinary drug investigation in
raw milk, based on QuECHERS extraction followed by RP-HPLC-UV analysis. Use of this
benchtop system was motivated by its availability and moderate cost relatively to other sophisticated
methods such as LC-MS which are more efficient.
Methods:
This developed method has been optimized and then after validation according to EU
legislation, it demonstrated good linearity with R²>0.997, acceptable peak resolution within a short
time (<9.5 min) and good recovery of the analyzed drugs (OXY, ALZ and IVR, respectively
87.08, 99.02 and 92.01 %). Additionally, we applied the method to the analysis of cow milk, collected
in Nouakchott, capital of Mauritania.
Results:
The obtained results indicated a mixed level of drug use according to targeted molecules.
In 42% of sampled farms, the anti-parasitics IVR and ALZ were detected whereas the antibiotic
OXY was detected in 50%.
Conclusion:
This investigation shows that 17% of the sampled farms exceeded European standards
for IVR drug.
Extended liquid chromatography high resolution mass spectrometry screening method for veterinary drug, pesticide and human pharmaceutical residues in aquaculture fish