Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm
in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic
infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of
isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic
evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of
P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey
under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to
azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected
by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear
result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass
Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic
compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents
which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability
to reduce the production of biofilm formation rate.