Single-staining flow cytometry approach using SYTOX™ green to describe electroporation effects on Escherichia coli

Food Control ◽  
2022 ◽  
Vol 132 ◽  
pp. 108488
Author(s):  
Elena Zand ◽  
Felix Schottroff ◽  
Christoph Schoenher ◽  
Katharina S. Zimmermann ◽  
Marija Zunabovic-Pichler ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry

DNA replication in Escherichia coli gyrB(Ts) mutants analysed by flow cytometry

1991 ◽  
Vol 142 (2-3) ◽  
pp. 223-227 ◽  
Author(s):  
U. von Freiesleben ◽  
K.V. Rasmussen
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Dna Replication

scholarly journals Occurrence of Escherichia coli O157:H7 in river water determined by flow cytometry.

1998 ◽  
Vol 13 (2) ◽  
pp. 77-83 ◽  
Author(s):  
YASUNOBU TANAKA ◽  
MASATO YOSHIMITSU ◽  
NOBUYASU YAMAGUCHI ◽  
KATSUJI TANI ◽  
MASAO NASU
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
River Water ◽  
Escherichia Coli O157

Determination of Internalization of Chromium Oxide Nano-Particles in Escherichia coli by Flow Cytometry

2011 ◽  
Vol 7 (1) ◽  
pp. 168-169 ◽  
Author(s):  
Imrana Khatoon ◽  
Poornima Vajpayee ◽  
Gulshan Singh ◽  
AlokK. Pandey ◽  
Alok Dhawan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Chromium Oxide ◽  
Nano Particles

scholarly journals Development and Characterization of a Fluorescent-Bacteriophage Assay for Detection of Escherichia coli O157:H7

1999 ◽  
Vol 65 (4) ◽  
pp. 1397-1404 ◽  
Author(s):  
Lawrence Goodridge ◽  
Jinru Chen ◽  
Mansel Griffiths
Keyword(s):  
Escherichia Coli ◽  
Flow Cytometry ◽  
Immunomagnetic Separation ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Lower Detection ◽  
Unreliable Method ◽  
Direct Epifluorescent Filter Technique ◽  
Bacterial Concentrations

ABSTRACT In this paper we describe evaluation and characterization of a novel assay that combines immunomagnetic separation and a fluorescently stained bacteriophage for detection of Escherichia coliO157:H7 in broth. When it was combined with flow cytometry, the fluorescent-bacteriophage assay (FBA) was capable of detecting 104 cells/ml. A modified direct epifluorescent-filter technique (DEFT) was employed in an attempt to estimate bacterial concentrations. Using regression analysis, we calculated that the lower detection limit was between 102 and 103cells/ml; however, the modified DEFT was found to be an unreliable method for determining bacterial concentrations. The results of this study show that the FBA, when combined with flow cytometry, is a sensitive technique for presumptive detection of E. coliO157:H7 in broth cultures.

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