To analyze the formation of fatty acid (FA) and trans fatty acids (TFAs) in camellia oil, the method of quantitative analysis of FA was investigated by gas chromatography (GC). 15 kinds of fatty acids methyl esters (FAMEs) standards have been referred; the database used for external and internal standard analysis method was established. The correlation coefficient (r2) of all the linear regression equations both external and internal standard curves were more than 0.99, the recovery of all FAMEs standards from 92.01% to 106.7%, the coefficient of variation (CV) of the intra-day from 2.966% to 6.302%. Five FA, i.e. C16:0, C18:0, C18:1-9c, C18:2-9c12c, C18:3-9c12c15c, have been discovered in raw camellia oil. The content was 8.732, 2.168, 81.44, 8.925 and 0.7299 g/100g, respectively. Six kinds of FA have been formatted, i.e. C16:0, C18:0, C18:1-9t, C18:1-9c, C18:2-9c12c, C18:3-9c12c15c in heated camellia oil (270oC, 1 h) with the content of 8.341, 2.101, 1.931, 71.72, 4.636, 0.4680 g/100g, respectively. These values suggested that an extensive change has been occurred in the formation of FA of camellia oil after heated.