Rapid HPLC/MS/MS analysis of phenolic content and profile for mead quality assessment

Food Control ◽  
2021 ◽  
pp. 108737
Author(s):  
Lenka Česlová ◽  
Kateřina Pravcová ◽  
Miroslava Juričová ◽  
Jan Fischer
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.


Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1569
Author(s):  
Gabriele Carullo ◽  
Francesca Scarpelli ◽  
Emilia Lucia Belsito ◽  
Paolino Caputo ◽  
Cesare Oliviero Rossi ◽  
...  

The aim of this investigation was to prepare two solid mixtures containing a soluble polymorph of (+)-catechin and mucic (MUC) or tartaric (TAR) acids as new leavening agents. The solid mixtures were based on a polymorph of (+)-catechin, characterized through Powder X-ray Diffraction (PXRD) analysis and assayed in in vitro antioxidant and solubility assays. The dough samples were studied by dynamic rheological tests, while muffins were studied through Headspace Solid-Phase Microextraction (HS-SPME)/Gas Chromatography-Mass Spectrometry (GC-MS) analysis to identify volatile compounds, in vitro tests to evaluate antioxidant properties, and sensory analyses. TAR powder showed a solubility in water almost one order of magnitude increased with respect to commercial (+)-catechin (40.0 against 4.6 mg mL−1) and increased antioxidant performances. In particular, TAR showed total phenolic content (TPC) and total antioxidant capacity (TAC) values of 0.0298 ± 0.021 and 0.0081 ± 0.0009 meq CT/g, while MUC showed better results in terms of 2,2-diphenyl-1-picrylhydrazyl) acid (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 0.518 ± 0.015 and 0.112 ± 0.010 mg/mL, respectively. MS analysis identified different compounds derived from the lipid oxidation process. Muffins obtained using both powders showed interesting outcomes regarding dough process and appreciable appearance/olfactory/taste/texture profiles. Muffins obtained from TAR-based mixture showed also a total phenolic content of 0.00175 meq CT/g muffin, and almost two times improved TAC and scavenger activity against DPPH radical. The formulated powders could be used as suitable health-promoting ingredients in the food industry.


PROTEOMICS ◽  
2011 ◽  
Vol 11 (6) ◽  
pp. 1114-1124 ◽  
Author(s):  
Marianne Sandin ◽  
Morten Krogh ◽  
Karin Hansson ◽  
Fredrik Levander

OENO One ◽  
2020 ◽  
Vol 54 ◽  
Author(s):  
Enrico Serni ◽  
Ulrich Pedri ◽  
Josep Valls ◽  
Christoff Sanoll ◽  
Nikola Dordevic ◽  
...  

Aim: this work gives a chemical description and sensory evaluation of several Pinot noir wines from different parts of Italy. For three subsequent years (2016-2018) the wine samples were submitted for in an Italian annual national Pinot noir competition aiming to define the best Pinot noir red wine from Italy. All of the wine samples were 3-years old (from vinification) at the moment they were analysed and evaluated; they were also registered for the competition the same year they were put on the market.Methods and results: all the wines were evaluated by a tasting panel composed of oenologists and wine experts, using the overall sensory quality as the descriptor. For the chemical screening, standard oenological chemical parameters (total acidity, colour, alcohol degree, total phenolic content, tannin indexes, etc.) and the content of the most abundant phenolic molecules by means of LC-MS analysis (triple quadrupole with internal standards) were determined. Pinot noir red wines produced from different parts of Italy showed a high variability for most standard wine chemical parameters considered, while the content of most single phenolic constituents was more retained and consistent with data from literature; except for t-resveratrol, which was significantly higher in our analyses, and delphinidin-3-glucoside, which was lower. Moreover, changes regarding the corresponding wines from the three vintages were noted. A correlation between the chemical parameters and the tasting panel results was also attempted. The results from a statistical analysis confirmed that alcoholic content, malvidin-3-glucoside and total anthocyanins had the highest positive impact on quality scores, while gallic acid, color tonality and total phenolic content had the highest negative.Conclusions: our results indicate that most wine producers have a conservative attitude with very slight differences found in the corresponding wines over the three years of investigation. The strong effects of agronomical, winemaking and ageing processes on chemical and sensorial features of Pinot noir red wines from Italy were also clearly shown. Compared to other monovarietal Pinot noir red wines from the same temperate area, single polyphenol content tended to be more retained than most standard chemical parameters.Significance and impact of the study: an overall quality assessment of a monovarietal wine, with its typicity as the main goal of a sensorial investigation, appears to be different from an objective quality assessment carried out by trained professional personnel using single standardised descriptors. Positive and negative correlations exist between sensorial judgement and chemical parameters, and the multiple linear regression model revealed relationships between the wine score and the set of the most important wine score description parameters.


2021 ◽  
Vol 9 (5) ◽  
pp. 671-680
Author(s):  
Mohd Sadiq S. Zarger ◽  
◽  
Nida Akhtar ◽  
Fehmeeda Khatoon ◽  
◽  
...  

India is one of the richest known in terms of biodiversity, especially the northern India Jammu and Kashmir one of the beautiful geographical regions of the world is hub of medicinal plants. Two medicinal plants namely Glycyrrhiza glabra L. and Malwa sylvestris L. were selected for the study. The aim of this research was to investigate presence of preliminary phytochemicals and to determine the total flavonoid and phenolic content. Isolation of phytoconstituents was analyzed through GC-MS. The solvent used was methanol and for the organic solvent extraction Soxhlet apparatus was used. The extract was then separated by GC-MS through Shimadzu (2010) model. Preliminary phytochemical screening of the methanolic leaves extracts of G. glabra L. and M. sylvestris L. was carried out using standard methods which showed alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, proteins, phenols and anthraquinones respectively. Total phenolic content derived were 15.5mg/gm, 16.2mg/gm, 13.6mg/gm, 18.3gm/gm, 14.4mg/gm 13.6mg/gm, 24.3mg/gm, 19.5mg/gm, 21.3mg/gm and total flavonoid content derived were 10.2mg/gm, 23.4mg/gm, 18.5mg/gm, 19.3mg/gm, 24.3mg/gm, 19.8mg/gm, 22.4mg/gm, 25.6mg/gm, 16.2mg/gm respectively. GC-MS analysis showed forty two chemicals compounds from G. glabra L. and forty from M. sylvestris L. there were some major compounds noticed in both extracts which provided the evidence that these plants contain medicinally important bioactive compounds which can be used traditionally for the treatment of different kinds of fungal and bacterial diseases.


1997 ◽  
Vol 24 (7) ◽  
pp. 496-505 ◽  
Author(s):  
E. S. GROSSMAN ◽  
J. M. MATEJKA
Keyword(s):  

PsycCRITIQUES ◽  
2006 ◽  
Vol 51 (14) ◽  
Author(s):  
Howard N. Garb
Keyword(s):  

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