Invasive potential of sub-lethally injured Campylobacter jejuni and Salmonella Typhimurium during storage in chicken meat juice

Food Control ◽  
2022 ◽  
pp. 108823
Author(s):  
Gayani Weerasooriya ◽  
Samiullah Khan ◽  
Kapil K. Chousalkar ◽  
Andrea R. McWhorter
Keyword(s):  
Salmonella Typhimurium ◽  
Campylobacter Jejuni ◽  
Chicken Meat ◽  
Invasive Potential ◽  
Meat Juice

scholarly journals The Interplay between Campylobacter and the Caecal Microbial Community of Commercial Broiler Chickens over Time

2021 ◽  
Vol 9 (2) ◽  
pp. 221
Author(s):  
Ilaria Patuzzi ◽  
Massimiliano Orsini ◽  
Veronica Cibin ◽  
Sara Petrin ◽  
Eleonora Mastrorilli ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Control Strategies ◽  
Chicken Meat ◽  
Control Measures ◽  
Microbial Network ◽  
Gut Colonization ◽  
Commercial Broiler ◽  
Zoonotic Bacteria ◽  
Human Infections

Campylobacter is the most frequent foodborne zoonotic bacteria worldwide, with chicken meat being overwhelmingly the most important reservoir for human infections. Control measures implemented at the farm level (i.e., biosecurity or vaccination), which have been successfully applied to limit other pathogens, such as Salmonella, have not been effective in reducing Campylobacter occurrence. Thus, new approaches are needed to fully understand the ecological interactions of Campylobacter with host animals to effectively comprehend its epidemiology. The objective of this study was to analyse longitudinally the gut microbiota composition of Campylobacter-infected and non-infected farms to identify any difference that could potentially be indicative of gut colonization by Campylobacter spp. Differences in the colonization rate and timing were observed at the farms that became positive for Campylobacter jejuni over the investigated time points, even though in positive tests, the occurrence of Campylobacter jejuni gut colonization was not observed before the second week of the life of the birds. Significant differences were observed in the abundances of specific bacterial taxa between the microbiota of individuals belonging to farms that became Campylobacter positive during the study and those who remained negative with particular reference to Bacteroidales and Clostridiales, respectively. Moreover, Campylobacter colonization dramatically influenced the microbiota richness, although to a different extent depending on the infection timing. Finally, a key role of Faecalibacterium and Lactobacillus genera on the Campylobacter microbial network was observed. Understanding the ecology of the Campylobacter interaction with host microbiota during infection could support novel approaches for broiler microbial barrier restoration. Therefore, evidence obtained through this study can be used to identify options to reduce the incidence of infection at a primary production level based on the targeted influence of the intestinal microbiota, thus helping develop new control strategies in order to mitigate the risk of human exposure to Campylobacter by chicken meat consumption.

scholarly journals Infection and cross-infection in a Paediatric Gastro-enteritis unit

Curationis ◽  
1989 ◽  
Vol 12 (3/4) ◽  
Author(s):  
Jean Bowen Jones
Keyword(s):  
Nosocomial Infection ◽  
Salmonella Typhimurium ◽  
Campylobacter Jejuni ◽  
Cross Infection ◽  
Enteric Pathogens ◽  
Academic Hospital ◽  
Viral Pathogens ◽  
Contributory Factors ◽  
Shigella Spp ◽  
The Mean

A two month study to investigate the incidence o f nosocomial infection was conducted in a paediatric gastroenteritis ward o f a black academic hospital. Enteric pathogens were identified on admission in 61 (47,2%) o f 129 patients; 56 bacterial and 25 viral. Six per cent o f patients had a combination o f bacterial and viral pathogens. Enteric pathogens most frequently identified on admission were Campylobacter jejuni in 22%, Rotavirus in 19,3%, EPEC in 10,8% and Shigella spp. in 6,9% patients. Twenty six (20%) patients had more than 1 enteric pathogen. The nosocomial infection rate was recorded at 17,1%. EPEC occurred most commonly in 5,3% patients, Salmonella typhimurium in 4,6% and Shigella spp. in 2,3%. Nosocomial infections increased the mean length o f hospital stay from 7,2- 20,2 days. Contributory factors to the spread o f nosocomial infection were the unsatisfactory methods o f bathing patients and giving naso-gastric feeds.

scholarly journals Correlation between Genotypic Diversity, Lipooligosaccharide Gene Locus Class Variation, and Caco-2 Cell Invasion Potential of Campylobacter jejuni Isolates from Chicken Meat and Humans: Contribution to Virulotyping

2009 ◽  
Vol 75 (13) ◽  
pp. 4277-4288 ◽  
Author(s):  
Ihab Habib ◽  
Rogier Louwen ◽  
Mieke Uyttendaele ◽  
Kurt Houf ◽  
Olivier Vandenberg ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Genotypic Diversity ◽  
Chicken Meat ◽  
Pcr Screening ◽  
Class A ◽  
Invasion Potential ◽  
Cell Surface Carbohydrate ◽  
Significant Difference ◽  
Class B ◽  
Class C

ABSTRACT Significant interest in studying the lipooligosaccharide (LOS) of Campylobacter jejuni has stemmed from its potential role in postinfection paralytic disorders. In this study we present the results of PCR screening of five LOS locus classes (A, B, C, D, and E) for a collection of 116 C. jejuni isolates from chicken meat (n = 76) and sporadic human cases of diarrhea (n = 40). We correlated LOS classes with clonal complexes (CC) assigned by multilocus sequence typing (MLST). Finally, we evaluated the invasion potential of a panel of 52 of these C. jejuni isolates for Caco-2 cells. PCR screening showed that 87.1% (101/116) of isolates could be assigned to LOS class A, B, C, D, or E. Concordance between LOS classes and certain MLST CC was revealed. The majority (85.7% [24/28]) of C. jejuni isolates grouped in CC-21 were shown to express LOS locus class C. The invasion potential of C. jejuni isolates possessing sialylated LOS (n = 29; classes A, B, and C) for Caco-2 cells was significantly higher (P < 0.0001) than that of C. jejuni isolates with nonsialylated LOS (n = 23; classes D and E). There was no significant difference in invasiveness between chicken meat and human isolates. However, C. jejuni isolates assigned to CC-206 (correlated with LOS class B) or CC-21 (correlated with LOS class C) showed statistically significantly higher levels of invasion than isolates from other CC. Correlation between LOS classes and CC was further confirmed by pulsed-field gel electrophoresis. The present study reveals a correlation between genotypic diversity and LOS locus classes of C. jejuni. We showed that simple PCR screening for C. jejuni LOS classes could reliably predict certain MLST CC and add to the interpretation of molecular-typing results. Our study corroborates that sialylation of LOS is advantageous for C. jejuni fitness and virulence in different hosts. The modulation of cell surface carbohydrate structure could enhance the ability of C. jejuni to adapt to or survive in a host.

scholarly journals Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

2013 ◽  
Vol 79 (12) ◽  
pp. 3875-3878 ◽  
Author(s):  
Sonja Kittl ◽  
Bożena M. Korczak ◽  
Lilian Niederer ◽  
Andreas Baumgartner ◽  
Sabina Buettner ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Human Infection ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Production Chain ◽  
Content Type ◽  
Resistance Patterns ◽  
Retail Meat ◽  
Retail Chicken Meat ◽  
Antibiotic Resistance Patterns

ABSTRACTMultilocus sequence typing (MLST) and antibiotic resistance patterns ofCampylobacter jejuniandCampylobacter colifrom retail chicken meat showed high overlap with isolates collected at slaughterhouses, indicating little selection along the production chain. They also showed significant common sequence types with human clinical isolates, revealing chicken meat as a likely source for human infection.

scholarly journals Epidemiological investigations onCampylobacter jejuniin households with a primary infection

1984 ◽  
Vol 93 (2) ◽  
pp. 325-332 ◽  
Author(s):  
J. Oosterom ◽  
C. H. den Uyl ◽  
J. R. J. Bänffer ◽  
J. Huisman
Keyword(s):  
Campylobacter Jejuni ◽  
Primary Infection ◽  
Index Patient ◽  
Chicken Meat ◽  
Circumstantial Evidence ◽  
Common Source ◽  
Household Contacts ◽  
Control Subjects ◽  
Source Of Infection ◽  
Spread Of Infection

SummaryFifty-four Rotterdam patients in which a primary infection withCampylobacter jejunihad been detected (index patients) were compared with 54 control subjects with regard to the consumption and preparation of foods 7 days before onset of illness and the keeping of pet animals. Significantly more index patients than controls had eaten chicken meat (47v. 29;P= 0·0002), particularly at barbecues (14v. 2;P= 0·0015). Marginally more index patients had eaten pork (47v. 39;P= 0·048) or inadequately heated meat (13v. 8), though in the last case numbers were too small to be statistically significant. The consumption of beef or mutton and outdoor eating (other than at barbecues) were essentially the same in both groups. There was no significant association with the keeping of pet animals, although a few more index patients had cage birds than controls (18v. 12).Twenty-one (15%) of 130 household contacts of index patients also suffered from diarrhoea during the same period. Circumstantial evidence pointed to a common source of infection with the index patient in 13 instances (nine households) and probable intrafamilial spread of infection in six instances.Campylobacters were isolated from one of 110 swabs of kitchen work surfaces and eight of 107 swabs taken from lavatory bowls in index households.

scholarly journals Prevalence and susceptibility to antibiotics from Campylobacter jejuni and Campylobacter coli isolated from chicken meat in southern Benin, West Africa

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Sylvain Daton Kouglenou ◽  
Alidehou Jerrold Agbankpe ◽  
Victorien Dougnon ◽  
Armando Djiyou Djeuda ◽  
Esther Deguenon ◽  
...  
Keyword(s):  
West Africa ◽  
Campylobacter Jejuni ◽  
Chicken Meat ◽  
Campylobacter Coli ◽  
Southern Benin

Effect of chicken meat environment on gene expression of Campylobacter jejuni and its relevance to survival in food

2011 ◽  
Vol 145 ◽  
pp. S111-S115 ◽  
Author(s):  
Małgorzata Ligowska ◽  
Marianne Thorup Cohn ◽  
Richard A. Stabler ◽  
Brendan W. Wren ◽  
Lone Brøndsted
Keyword(s):  
Gene Expression ◽  
Campylobacter Jejuni ◽  
Chicken Meat

scholarly journals Two rapid diagnostic procedures for the identification of Campylobacter jejuni/coli in food matrix

2011 ◽  
Vol 23 (No. 3) ◽  
pp. 121-125
Author(s):  
T. Jeleník ◽  
Z. Šabatková ◽  
K. Demnerová ◽  
J. Pazlarová
Keyword(s):  
Chronic Illness ◽  
Detection Limit ◽  
Campylobacter Jejuni ◽  
Positive Reaction ◽  
Diagnostic Procedures ◽  
Chicken Meat ◽  
Food Matrix ◽  
Selective Enrichment ◽  
Campylobacter Species ◽  
Campylobacter Spp

Campylobacter species, in particular C. jejuni and C. coli, cause infections which vary in symptoms, ranging from asymptomatic to severe chronic illness. The only ISO method for the detection of Campylobacter spp. until now has been the cultivation by selective enrichment and distinct conditions of growth taking several days to complete. We compared the Singlepath<sup>&reg;</sup> Campylobacter test which involved 24 h of enrichment in Bolton broth, with PCR-based identification. Chicken meat salad with mayonnaise was spiked with C. jejuni and C. coli and the detection limit was determined. PCR provided the same detection limit of 10<sup>2</sup> CFU/ml for both strains. The immunotest Singlepath<sup>&reg;</sup> was positive with C. jejuni only, the quantity of cells being 10<sup>3 </sup>CFU/ml. C. coli was undetectable by Singlepath<sup>&reg;</sup>, even the concentration of 10<sup>5 </sup>did not reveal a positive reaction. &nbsp;

scholarly journals Active Packaging of Immobilized Zinc Oxide Nanoparticles Controls Campylobacter jejuni in Raw Chicken Meat

2020 ◽  
Vol 86 (22) ◽  
Author(s):  
Mohammed J. Hakeem ◽  
Jinsong Feng ◽  
Azadeh Nilghaz ◽  
Luyao Ma ◽  
Hwai Chuin Seah ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Shelf Life ◽  
Campylobacter Jejuni ◽  
Zinc Oxide Nanoparticles ◽  
Chicken Meat ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Content Type ◽  
Active Food Packaging

ABSTRACT Zinc oxide nanoparticles (ZnO NPs) are regarded as a safe and stable antimicrobial that can inactivate bacteria by several potential working mechanisms. We aimed to incorporate ZnO NPs into packaging material to control Campylobacter in raw chicken meat. ZnO NPs were first incorporated into three-dimensional (3D) paper tubes to identify the lethal concentration against Campylobacter jejuni, which was selected as the working concentration to develop 2D functionalized absorbing pads by an ultrasound-assisted dipping technique. The functionalized pad was placed underneath raw chicken meat to inactivate C. jejuni and the predominant chicken microbiota at 4°C within 8 days of storage. Immobilized ZnO NPs at 0.856 mg/cm2 reduced C. jejuni from ∼4 log CFU/25 g raw chicken meat to an undetectable level after 3 days of storage. Analysis by inductively coupled plasma-optical emission spectroscopy showed that the Zn level increased from 0.02 to 0.17 mg/cm2 in treated raw chicken meat. Scanning electron microscopy validated the absence of nanoparticle migration onto raw chicken meat after treatment. Inactivation of C. jejuni was associated with the increase of lactic acid produced by Lactobacillus in raw chicken meat in a pH-dependent manner. Less than 5% of Zn2+ was released from ZnO NPs at neutral pH, while up to 88% was released when the pH was <3.5 within 2 days. Whole-transcriptome sequencing (RNA-Seq) analysis demonstrated a broad effect of ZnO NPs on genes involved in various cellular developmental processes as annotated by gene ontology. Taken together, the results indicate that functionalized absorbing pads inactivated C. jejuni in raw chicken meat by immobilized ZnO NPs along with the controllable released Zn2+. IMPORTANCE Prevalence of Campylobacter in raw poultry remains a major food microbiological safety challenge. Novel mitigation strategies are required to ensure the safety and quality of poultry products. Active food packaging can control pathogens without directly adding antimicrobials into the food matrix and extend the food’s shelf life. The functionalized absorbing pad with ZnO NPs developed in this study was able to inactivate C. jejuni in raw chicken meat and keep the meat free from C. jejuni contamination during shelf life without any observed migration of nanoparticles. The controllable conversion of immobilized ZnO NPs to free Zn2+ makes this approach safe and eco-friendly and paves the way for developing a novel intervention strategy for other high-risk foods. Our study applied nanotechnology to exploit an effective approach for Campylobacter control in raw chicken meat products.

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