The evolution and functional characterization of miiuy croaker cytosolic gene LGP2 involved in immune response

2016 ◽  
Vol 58 ◽  
pp. 193-202 ◽  
Author(s):  
Jingjing Han ◽  
Yanjin Wang ◽  
Qing Chu ◽  
Tianjun Xu
2022 ◽  
Vol 127 ◽  
pp. 104293
Author(s):  
Zishu Huang ◽  
Yueling Zhang ◽  
Xiaoyu Zheng ◽  
Zhuoyan Liu ◽  
Defu Yao ◽  
...  

2012 ◽  
Vol 189 (2) ◽  
pp. 935-945 ◽  
Author(s):  
Larissa N. A. Longhi ◽  
Rosiane M. da Silva ◽  
Márcia C. Fornazim ◽  
Maria C. Spago ◽  
Rômulo T. D. de Oliveira ◽  
...  

2016 ◽  
Vol 40 (4) ◽  
pp. 491-508 ◽  
Author(s):  
Claire Rosnoblet ◽  
Hervé Bègue ◽  
Cécile Blanchard ◽  
Carole Pichereaux ◽  
Angélique Besson‐Bard ◽  
...  

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei-Chen Lin ◽  
Chia-Yun Tsai ◽  
Jian-Ming Huang ◽  
Shang-Rung Wu ◽  
Lichieh Julie Chu ◽  
...  

Abstract Background Pathogenic protozoans use extracellular vesicles (EVs) for intercellular communication and host manipulation. Acanthamoeba castellanii is a free-living protozoan that may cause severe keratitis and fatal granulomatous encephalitis. Although several secreted molecules have been shown to play crucial roles in the pathogenesis of Acanthamoeba, the functions and components of parasite-derived EVs are far from understood. Methods Purified EVs from A. castellanii were confirmed by electron microscopy and nanoparticle tracking analysis. The functional roles of parasite-derived EVs in the cytotoxicity to and immune response of host cells were examined. The protein composition in EVs from A. castellanii was identified and quantified by LC-MS/MS analysis. Results EVs from A. castellanii fused with rat glioma C6 cells. The parasite-derived EVs induced an immune response from human THP-1 cells and a cytotoxic effect in C6 cells. Quantitative proteomic analysis identified a total of 130 proteins in EVs. Among the identified proteins, hydrolases (50.2%) and oxidoreductases (31.7%) were the largest protein families in EVs. Furthermore, aminopeptidase activities were confirmed in EVs from A. castellanii. Conclusions The proteomic profiling and functional characterization of EVs from A. castellanii provide an in-depth understanding of the molecules packaged into EVs and their potential mechanisms mediating the pathogenesis of this parasite.


Gene ◽  
2019 ◽  
Vol 690 ◽  
pp. 21-29 ◽  
Author(s):  
Xinfang Mao ◽  
Xin Xu ◽  
Xiaoxia Yang ◽  
Zaixin Li ◽  
Jianhua Yang ◽  
...  

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