Colonization characteristics of pioneer surface-associated eukaryotes during natural biofilm formation on PDMS-based composites via 18S rRNA gene sequencing methods

Monitoring phytoplankton community underpins our understanding of water quality and ecological functions. In this study, we approached phytoplankton abundance, community composition, and diversity by both microscopy and 18S rRNA gene sequencing. Environmental variances influencing the phytoplankton were evaluated as well. There were 6 phyla and 62 species identified by microscopy, and the diversity index Shannon-Wiener and evenness index Pielou index indicated phytoplankton community had high diversity; however, the high density of dominance genus suggested that our research region had potential red tide effects. The canonical correspondence analysis illustrated that suspended solids, phosphate and temperature were three major factors that affected the distribution and components of phytoplankton community. The DNA barcoding sequencing of 18S rRNA gene supported the main results via microscopic methods while providing more identified community components, which implied that 18S rRNA gene sequencing can be used as a supplemental method for fast ecological assessment of phytoplankton community.

Download Full-text

Association between diet and rumen microbiota in wild roe deer

FEMS Microbiology Letters ◽

10.1093/femsle/fnz060 ◽

2019 ◽

Vol 366 (6) ◽

Cited By ~ 1

Author(s):

Robert Wilson ◽

Kjartan Østbye ◽

Inga Leena Angell ◽

Knut Rudi

Keyword(s):

18S Rrna ◽

Roe Deer ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

18S Rrna Gene ◽

Rrna Gene ◽

Rumen Microbiota ◽

Dietary Components ◽

Rrna Gene Sequencing ◽

Insight Into

ABSTRACT The association between diet and the rumen microbiota for wild animals remains largely unexplored. Here, we explored this association using a combination of 16S rRNA gene sequencing to determine the prokaryote microbiota and 18S rRNA gene sequencing to determine the dietary components for wild roe deer. These analyses revealed a wide diversity of dietary components, with over-representation of Bacteroidetes for the diet-correlating bacteria. Ruminococcus, on the other hand, dominated the stable diet-independent part of the microbiota. Taken together, the combination of 16S and 18S rRNA gene analyses provide novel insight into rumen microbiota ecology.

Download Full-text

Diversity and distribution of nematodes associated with terrestrial slugs in the Western Cape Province of South Africa

Journal of Helminthology ◽

10.1017/s0022149x11000277 ◽

2011 ◽

Vol 86 (2) ◽

pp. 215-221 ◽

Cited By ~ 16

Author(s):

J.L. Ross ◽

E.S. Ivanova ◽

W.F. Sirgel ◽

A.P. Malan ◽

M.J. Wilson

Keyword(s):

South Africa ◽

18S Rrna ◽

Parasitic Nematode ◽

Gene Sequencing ◽

18S Rrna Gene ◽

Rrna Gene ◽

Western Cape ◽

Western Cape Province ◽

Rrna Gene Sequencing ◽

Three New Species

AbstractA survey of nematodes associated with native and introduced species of terrestrial slugs was conducted in the Western Cape Province of South Africa, in order to gather new data regarding diversity and distribution. A total of 521 terrestrial slugs were collected from 35 localities throughout the Western Cape. All slugs were dissected and examined for the presence of internal nematodes. Extracted nematodes were identified using a combination of molecular (18S rRNA gene sequencing) and morphological techniques. Nematodes were found parasitizing slugs at 14 of the 35 sites examined, amounting to 40% of sample sites. Of all slugs, 6% were infected with nematodes. A total of seven species of nematode were identified in the province, includingAgfa flexilis,Angiostomasp.,Phasmarhabditissp. SA1,Phasmarhabditissp. SA2,Caenorhabditis elegans,Panagrolaimussp. andRhabditissp. Of these species, four were thought to be parasitic to slugs (A. flexilis, Angiostomasp.,Phasmarhabditissp. SA1 andPhasmarhabditissp. SA2), as opposed to forming necromenic or phoretic associations. Three new species of slug-parasitic nematode were identified during this study (Angiostomasp.,Phasmarhabditissp. SA1 andPhasmarhabditissp. SA2).

Download Full-text