Prevalence of multidrug resistant Salmonella spp. in dairy products with the evaluation of the inhibitory effects of ascorbic acid, pomegranate peel extract, and D-tryptophan against Salmonella growth in cheese

Author(s):  
Mahmoud Elafify ◽  
Wageh Sobhy Darwish ◽  
Marwa El-Toukhy ◽  
Basma M. Badawy ◽  
Rehab E. Mohamed ◽  
...  
2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Maedeh Nejati ◽  
Leila Fozouni ◽  
Mehrali Mahmoodjanloo

Background: Gyms are among the places where various human infections can spread. One of the most important strategies to prevent infection spread among athletes is the use of strong and effective disinfectants. Objectives: The aim of this study was to evaluate and compare the antiseptic properties of chlorhexidine gluconate and pomegranate peel extract on staphylococcal isolates from sports mats. Methods: This descriptive-analytical study was performed on 22 Staphylococcus aureus, and Staphylococcus epidermidis isolates from a total of 105 sports mat samples. After identification of multi-drug resistant (MDR) staphylococcal isolates, the antimicrobial effect of chlorhexidine gluconate 20% and pomegranate peel extract was evaluated by broth microdilution according to the instructions of the Clinical and Laboratory Standards Institute. Results: Overall, 14 (66.7%) S. aureus and 8 (28.6%) S. epidermidis isolates resistant to azithromycin, linezolid, ciprofloxacin, vancomycin, and aztreonam were reported as MDR strains. The minimum inhibitory concentration (MIC) of chlorhexidine gluconate, which inhibited the growth of 90% of MDR S. aureus isolates was 250 μg/mL, which was 4-fold lower than the MIC of pomegranate peel extract (MIC90 ≥ 500μg/mL) and 2-fold lower against MDR S. epidermidis isolates (MIC90 ≤ 125 μg/mL). The MIC of chlorhexidine gluconate and pomegranate peel extract against S. aureus isolates differed significantly (P = 0.037). Conclusions: Compared to pomegranate peel extract, chlorhexidine gluconate has a higher antibacterial effect on MDR staphylococcal isolates from sports mats. Further research is required to verify the efficacy of this biocide for disinfection of gyms and sports clubs.


2019 ◽  
Vol 15 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Ahmed A. Haroun ◽  
Abdel-Tawab H. Mossa ◽  
Samia M.M. Mohafrash

Background: Funcionalized multi-walled carbon nanotubes (ox-MWCNTs) were used for the preparation of therapeutic nanoparticles for delivery of some bioactive compounds. Consequently, this work deals with the preparation of grafted MWCNTs with n-vinyl caprolactam in the presence of pomegranate peel extract (P. granatum), titanium dioxide (TiO2) and/or silver nanoparticeles and their toxic effects on male mice using in vivo biological examination (liver and kidney dysfunction biomarkers) and the histopathological analysis. Methods: P. granatum extract was immobilized onto functionalized MWCNTs using simple adsorption technique. Moreover, The prepared materials were analyzed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM). In vivo examination using liver and kidney dysfunction biomarkers was investigated. In addition, the histopathological study was carried out. Results: The ox-MWCNTs induced significant elevation in the liver enzymes including AST, ALT and ALP relative to the control group. While, the treatment with P. granatum extract only did not induce any change in the liver and kidney biomarkers. In other words, P. granatum extract loaded onto functionalized MWCNTs showed low effects on liver enzymes and kidney function biomarkers in the treated mice in comparison with ox-MWCNTs and extract separately. Moreover, histopathological analysis revealed that the P. granatum extract functionalized MWCNTs exhibited normal renal tissue with no histopathological alteration. Conclusion: The grafted MWCNTs with n-vinyl caprolactam in the presence of pomegranate peel extract (P. granatum), titanium dioxide (TiO2) and/or silver nanoparticeles were successfully prepared. SEM-micrographs showed complete coating of MWCNTs fiber with the extract. The prepared materials resulted in no toxic effects and the histopathological findings were confirmed by inflammation of the liver and kidney tissues.


2021 ◽  
Author(s):  
Sainan Zhou ◽  
Qing Zhang ◽  
Yuan Gao ◽  
Fang Liu ◽  
Wanxiu Cao ◽  
...  

Sargassum fusiforme together with the turmeric extract and pomegranate peel extract may effectively reduce fat accumulation and improve the disorder of intestinal flora in obese mice.


Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 705
Author(s):  
Asmaa M. El-Kady ◽  
Iman A. M. Abdel-Rahman ◽  
Samer S. Fouad ◽  
Khaled S. Allemailem ◽  
Taghrid Istivan ◽  
...  

Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of Giardia lamblia (G. lamblia) to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of Punica granatum (pomegranate) and evaluated its therapeutic efficacy in rats infected with G. lamblia. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing G. lamblia cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of G. lamblia cysts and trophozoites in feces and intestine respectively. Interestingly, the number of G. lamblia trophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controlling G. lamblia infections, which suggests that it could be a viable treatment option for giardiasis.


2021 ◽  
pp. 105145
Author(s):  
Relja Suručić ◽  
Maja Travar ◽  
Miroslav Petković ◽  
BiljanaTubić ◽  
Miloš P. Stojiljković ◽  
...  

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
A. Taherian ◽  
N. Esfandiari ◽  
S. Rouhani

Abstract Background Breast cancer is one of the most challenging cancers among women which is considered one of the most lethal cancers to this date. From the time that cancer has been discovered, finding the best therapeutic method is still an ongoing process. As a novel therapeutic method, nanomedicine has brought a vast number of materials that could versatilely be used as a drug carrier. The purpose of this study is to develop a novel black pomegranate peel extract loaded with chitosan-coated magnetic nanoparticles to treat breast cancer cells. Results The morphology and size distribution of the nanoparticles studied by dynamic light scattering, atomic force microscopy, scanning, and transitional electron microscopy showed the spherical shape of the nanoparticles and their promising size range. Studies by Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometer, and zeta sizer confirmed the synthesis, substantial crystallinity, magnetic potential of the nanoparticles, and their satisfactory stability. The DPPH assay revealed that the obtained black pomegranate peel extract has 60% free radical scavenging activity. The cytotoxicity studies by MTT and LDH assay carried out on NIH/3T3, MBA-MB-231, and 4T1 cells confirmed that the magnetic nanoparticles had no significant cytotoxicity on the cells. However, the drug-loaded nanoparticles could significantly eradicate cancerous cells which had more efficiency comparing to free drug. Furthermore, free drug and drug-loaded nanoparticles had no toxic effect on normal cells. Conclusion Owing to the results achieved from this study, the novel drug-loaded nanoparticles are compatible to be used for breast cancer treatment and could potentially be used for further in vivo studies.


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