Tuning hydrothermal pretreatment severity of wheat straw to match energy application scenarios

2022 ◽  
Vol 176 ◽  
pp. 114326
Author(s):  
Chenjun He ◽  
Jinguang Hu ◽  
Fei Shen ◽  
Mei Huang ◽  
Li Zhao ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Hydrothermal Pretreatment ◽  
Energy Application ◽  
Pretreatment Severity

scholarly journals Hydrothermal Pretreatment of Wheat Straw: Effects of Temperature and Acidity on Byproduct Formation and Inhibition of Enzymatic Hydrolysis and Ethanolic Fermentation

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 487
Author(s):  
Dimitrios Ilanidis ◽  
Stefan Stagge ◽  
Leif J. Jönsson ◽  
Carlos Martín
Keyword(s):  
Mass Spectrometry ◽  
Sulfuric Acid ◽  
Wheat Straw ◽  
High Performance ◽  
Enzymatic Saccharification ◽  
Hydrothermal Pretreatment ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzymatic Digestibility ◽  
Acid Catalyzed ◽  
Pretreatment Conditions

Biochemical conversion of wheat straw was investigated using hydrothermal pretreatment, enzymatic saccharification, and microbial fermentation. Pretreatment conditions that were compared included autocatalyzed hydrothermal pretreatment at 160, 175, 190, and 205 °C and sulfuric-acid-catalyzed hydrothermal pretreatment at 160 and 190 °C. The effects of using different pretreatment conditions were investigated with regard to (i) chemical composition and enzymatic digestibility of pretreated solids, (ii) carbohydrate composition of pretreatment liquids, (iii) inhibitory byproducts in pretreatment liquids, (iv) furfural in condensates, and (v) fermentability using yeast. The methods used included two-step analytical acid hydrolysis combined with high-performance anion-exchange chromatography (HPAEC), HPLC, ultra-high performance liquid chromatography-electrospray ionization-triple quadrupole-mass spectrometry (UHPLC-ESI-QqQ-MS), and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). Lignin recoveries in the range of 108–119% for autocatalyzed hydrothermal pretreatment at 205 °C and sulfuric-acid-catalyzed hydrothermal pretreatment were attributed to pseudolignin formation. Xylose concentration in the pretreatment liquid increased with temperature up to 190 °C and then decreased. Enzymatic digestibility was correlated with the removal of hemicelluloses, which was almost quantitative for the autocatalyzed hydrothermal pretreatment at 205 °C. Except for the pretreatment liquid from the autocatalyzed hydrothermal pretreatment at 205 °C, the inhibitory effects on Saccharomyces cerevisiae yeast were low. The highest combined yield of glucose and xylose was achieved for autocatalyzed hydrothermal pretreatment at 190 °C and the subsequent enzymatic saccharification that resulted in approximately 480 kg/ton (dry weight) raw wheat straw.

Effect of pretreatment severity on xylan solubility and enzymatic breakdown of the remaining cellulose from wheat straw

2007 ◽  
Vol 98 (10) ◽  
pp. 2034-2042 ◽  
Author(s):  
Mirjam A. Kabel ◽  
Gijs Bos ◽  
Jan Zeevalking ◽  
Alphons G.J. Voragen ◽  
Henk A. Schols
Keyword(s):  
Wheat Straw ◽  
Pretreatment Severity

scholarly journals Ensiling of wheat straw decreases the required temperature in hydrothermal pretreatment

2013 ◽  
Vol 6 (1) ◽  
pp. 116 ◽  
Author(s):  
Morten Ambye-Jensen ◽  
Sune Thomsen ◽  
Zsófia Kádár ◽  
Anne S Meyer
Keyword(s):  
Wheat Straw ◽  
Hydrothermal Pretreatment

Pressure drop, mechanic deformation, stabilization and scale-up of wheat straw fixed-beds during hydrothermal pretreatment: Experiments and modeling

2019 ◽  
Vol 360 ◽  
pp. 1587-1600 ◽  
Author(s):  
Wienke Reynolds ◽  
Marc Conrad ◽  
Sarah Mbeukem ◽  
Rainer Stank ◽  
Irina Smirnova
Keyword(s):  
Pressure Drop ◽  
Wheat Straw ◽  
Scale Up ◽  
Hydrothermal Pretreatment ◽  
Fixed Beds

scholarly journals Enzyme affinity to cell types in wheat straw (Triticum aestivum L.) before and after hydrothermal pretreatment

2013 ◽  
Vol 6 (1) ◽  
pp. 54 ◽  
Author(s):  
Mads AT Hansen ◽  
Budi J Hidayat ◽  
Kit K Mogensen ◽  
Martin D Jeppesen ◽  
Bodil Jørgensen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Wheat Straw ◽  
Cell Types ◽  
Triticum Aestivum L ◽  
Hydrothermal Pretreatment ◽  
Before And After

scholarly journals Effect of hydrothermal pretreatment on the structural changes of alkaline ethanol lignin from wheat straw

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Xue Chen ◽  
Hanyin Li ◽  
Shaoni Sun ◽  
Xuefei Cao ◽  
Runcang Sun
Keyword(s):  
Wheat Straw ◽  
Structural Changes ◽  
Hydrothermal Pretreatment

Effect of hydrothermal pretreatment severity on the pretreatment characteristics and anaerobic digestion performance of corn stover

2019 ◽  
Vol 289 ◽  
pp. 121646 ◽  
Author(s):  
Xiaocong Song ◽  
Akiber Chufo Wachemo ◽  
Liang Zhang ◽  
Tianqing Bai ◽  
Xiujin Li ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Corn Stover ◽  
Hydrothermal Pretreatment ◽  
Pretreatment Severity

scholarly journals The liquid fraction from hydrothermal pretreatment of wheat straw provides lytic polysaccharide monooxygenases with both electrons and H2O2 co-substrate

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Riin Kont ◽  
Ville Pihlajaniemi ◽  
Anna S. Borisova ◽  
Nina Aro ◽  
Kaisa Marjamaa ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Liquid Fraction ◽  
Reducing Power ◽  
Hydrothermal Pretreatment ◽  
Enzymatic Reactions ◽  
Lignocellulosic Substrate ◽  
Lytic Polysaccharide Monooxygenases ◽  
Polysaccharide Monooxygenases ◽  
Transient Activity ◽  
High Concentrations

Abstract Background Enzyme-aided valorization of lignocellulose represents a green and sustainable alternative to the traditional chemical industry. The recently discovered lytic polysaccharide monooxygenases (LPMOs) are important components of the state-of-the art enzyme cocktails for cellulose conversion. Yet, these monocopper enzymes are poorly characterized in terms of their kinetics, as exemplified by the growing evidence for that H2O2 may be a more efficient co-substrate for LPMOs than O2. LPMOs need external electron donors and one key question of relevance for bioprocess development is whether the required reducing power may be provided by the lignocellulosic substrate. Results Here, we show that the liquid fraction (LF) resulting from hydrothermal pretreatment of wheat straw supports LPMO activity on both chitin and cellulose. The initial, transient activity burst of the LPMO reaction was caused by the H2O2 present in the LF before addition of LPMO, while the steady-state rate of LPMO reaction was limited by the LPMO-independent production of H2O2 in the LF. H2O2 is an intermediate of LF oxidation as evidenced by a slow H2O2 accumulation in LF, despite high H2O2 production rates. This H2O2 scavenging ability of LF is important since high concentrations of H2O2 may lead to irreversible inactivation of LPMOs. Conclusions Our results support the growing understanding that fine-tuned control over the rates of H2O2 production and consumption in different, enzymatic and non-enzymatic reactions is essential for harnessing the full catalytic potential of LPMOs in lignocellulose valorization.

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