Overexpressing hemp salt stress induced transcription factor genes enhances tobacco salt tolerance

2022 ◽  
Vol 177 ◽  
pp. 114497
Author(s):  
Hao Liu ◽  
Huaran Hu ◽  
Kailei Tang ◽  
Muzammal Rehman ◽  
Guanghui Du ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Transcription Factor Genes

scholarly journals Association of transcription factor WRKY56 gene from Populus simonii × P. nigra with salt tolerance in Arabidopsis thaliana

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7291 ◽  
Author(s):  
Lei Wang ◽  
Wenjing Yao ◽  
Yao Sun ◽  
Jiying Wang ◽  
Tingbo Jiang
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Germination Rate ◽  
Wrky Transcription Factor ◽  
Biotic And Abiotic Stress ◽  
Populus Simonii ◽  
Reading Frame

The WRKY transcription factor family is one of the largest groups of transcription factor in plants, playing important roles in growth, development, and biotic and abiotic stress responses. Many WRKY genes have been cloned from a variety of plant species and their functions have been analyzed. However, the studies on WRKY transcription factors in tree species under abiotic stress are still not well characterized. To understand the effects of the WRKY gene in response to abiotic stress, mRNA abundances of 102 WRKY genes in Populus simonii × P. nigra were identified by RNA sequencing under normal and salt stress conditions. The expression of 23 WRKY genes varied remarkably, in a tissue-specific manner, under salt stress. Since the WRKY56 was one of the genes significantly induced by NaCl treatment, its cDNA fragment containing an open reading frame from P. simonii × P. nigra was then cloned and transferred into Arabidopsis using the floral dip method. Under salt stress, the transgenic Arabidopsis over-expressed the WRKY56 gene, showing an increase in fresh weight, germination rate, proline content, and peroxidase and superoxide dismutase activity, when compared with the wild type. In contrast, transgenic Arabidopsis displayed a decrease in malondialdehyde content under salt stress. Overall, these results indicated that the WRKY56 gene played an important role in regulating salt tolerance in transgenic Arabidopsis.

scholarly journals ThCOL2 Improves the Salt Stress Tolerance of Tamarix hispida

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaojin Lei ◽  
Bing Tan ◽  
Zhongyuan Liu ◽  
Jing Wu ◽  
Jiaxin Lv ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Cell Damage ◽  
Expression Patterns ◽  
Nacl Stress ◽  
Tamarix Hispida ◽  
Resistance Response ◽  
Expression Levels ◽  
Qrt Pcr

The CONSTANS-LIKE (COL) transcription factor has been reported to play important roles in regulating plant flowering and the response to abiotic stress. To clone and screen COL genes with excellent salt tolerance from the woody halophyte Tamarix hispida, 8 ThCOL genes were identified in this study. The expression patterns of these genes under different abiotic stresses (high salt, osmotic, and heavy metal) and abscisic acid (ABA) treatment were detected using quantitative real-time PCR (qRT-PCR). The expression levels of 8 ThCOL genes changed significantly after exposure to one or more stresses, indicating that these genes were all stress-responsive genes and may be involved in the stress resistance response of T. hispida. In particular, the expression level of ThCOL2 changed significantly at most time points in the roots and leaves of T. hispida under salt stress and after ABA treatments, which may play an important role in the response process of salt stress through a mechanism dependent on the ABA pathway. The recombinant vectors pROKII–ThCOL2 and pFGC5941–ThCOL2 were constructed for the transient transformation of T. hispida, and the transient infection of T. hispida with the pROKII empty vector was used as the control to further verify whether the ThCOL2 gene was involved in the regulation of the salt tolerance response of T. hispida. Overexpression of the ThCOL2 gene in plants under 150 mM NaCl stress increased the ability of transgenic T. hispida cells to remove reactive oxygen species (ROS) by regulating the activity of protective enzymes and promoting a decrease in the accumulation of O2– and H2O2, thereby reducing cell damage or cell death and enhancing salt tolerance. The ThCOL2 gene may be a candidate gene associated with excellent salt tolerance. Furthermore, the expression levels of some genes related to the ABA pathway were analyzed using qRT-PCR. The results showed that the expressions of ThNCED1 and ThNCED4 were significantly higher, and the expressions of ThNCED3, ThZEP, and ThAAO3 were not significantly altered in OE compared with CON under normal conditions. But after 24 h of salt stress, the expressions of all five studied genes all were lower than the normal condition. In the future, the downstream genes directly regulated by the ThCOL2 transcription factor will be searched and identified to analyze the salt tolerance regulatory network of ThCOL2.

scholarly journals AtCIPK16 Mediates Salt Stress Through Phytohormones and Transcription Factors

2020 ◽  
Author(s):  
Shanika L. Amarasinghe ◽  
Wenmian Huang ◽  
Nathan S. Watson-Haigh ◽  
Matthew Gilliham ◽  
Stuart J. Roy ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Food Production ◽  
Rna Seq ◽  
Productivity Losses ◽  
Global Food

AbstractSoil salinity causes large productivity losses for agriculture worldwide. “Next-generation crops” that can tolerate salt stress are required for the sustainability of global food production. Previous research in Arabidopsis thaliana aimed at uncovering novel factors underpinning improved plant salinity tolerance identified the protein kinase AtCIPK16. Overexpression of AtCIPK16 enhanced shoot Na+ exclusion and increased biomass in both Arabidopsis and barley. Here, a comparative transcriptomic study on Arabidopsis lines expressing AtCIPK16 was conducted in the presence and absence of salt stress, using an RNA-Seq approach, complemented by AtCIPK16 interaction and localisation studies. We are now able to provide evidence for AtCIPK16 activity in the nucleus. Moreover, the results manifest the involvement of a transcription factor, AtTZF1, phytohormones and the ability to quickly reach homeostasis as components important for improving salinity tolerance in transgenics overexpressing AtCIPK16. Furthermore, we suggest the possibility of both biotic and abiotic tolerance through AtCIPK16, and propose a model for the salt tolerance pathway elicited through AtCIPK16.

scholarly journals Transcription Factor CaSBP12 Negatively Regulates Salt Stress Tolerance in Pepper (Capsicum annuum L.)

2020 ◽  
Vol 21 (2) ◽  
pp. 444
Author(s):  
Huai-Xia Zhang ◽  
Wen-Chao Zhu ◽  
Xiao-Hui Feng ◽  
Jing-Hao Jin ◽  
Ai-Min Wei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Stress Tolerance ◽  
Severity Index ◽  
Pepper Plant ◽  
Plant Tolerance ◽  
Salt Stress Tolerance ◽  
Genome Database ◽  
Transcription Factor Genes ◽  
Pepper Genome

SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction, and stress response. However, little is known about the role of pepper SBP-box transcription factor genes in response to abiotic stress. Here, one of the pepper SBP-box gene, CaSBP12, was selected and isolated from pepper genome database in our previous study. The CaSBP12 gene was induced under salt stress. Silencing the CaSBP12 gene enhanced pepper plant tolerance to salt stress. The accumulation of reactive oxygen species (ROS) of the detached leaves of CaSBP12-silenced plants was significantly lower than that of control plants. Besides, the Na+, malondialdehyde content, and conductivity were significantly increased in control plants than that in the CaSBP12-silenced plants. In addition, the CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to salt stress with higher damage severity index percentage and accumulation of ROS as compared to the wild-type. These results indicated that CaSBP12 negatively regulates salt stress tolerance in pepper may relate to ROS signaling cascades.

scholarly journals Improved salt tolerance in transgenic tobacco by over-expression of poplar NAC13 gene

2019 ◽  
Author(s):  
Xuemei Zhang ◽  
Zihan Cheng ◽  
Kai Zhao ◽  
Renhua Li ◽  
Boru Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Transgenic Tobacco ◽  
Stress Responses ◽  
Gene Networks ◽  
Molecular Mechanisms ◽  
Physiological Characterization ◽  
Protein Functions ◽  
Forest Genetic

Background: NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. Methods: In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both tobacco and Arabidopsis. Results: Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that the transgenic tobacco enhanced salt tolerance, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress. Furthermore, evidence from yeast two-hybrid screening demonstrated that NAC13 protein functions as a transcriptional activator, with an activation domain located in the C-terminal region. Discussion: NAC13 gene plays an important role in response to salt stress in tobacco. Future studies are needed to shed light on molecular mechanisms of gene regulation and gene networks related to NAC13 gene in response to salt stress, which will provide a valuable theoretical basis for forest genetic breeding and resistant breeding.

scholarly journals BpTCP3 Transcription Factor Improves Salt Tolerance of Betula platyphylla by Reducing Reactive Oxygen Species Damage

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1633
Author(s):  
Li Ren ◽  
Fangrui Li ◽  
Jing Jiang ◽  
Huiyu Li
Keyword(s):  
Reactive Oxygen Species ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Reactive Oxygen ◽  
Betula Platyphylla ◽  
Oxygen Species ◽  
Salt Stress Response

The plant-specific transcription factors TEOSINTE BRANCHED1/CYCLO IDEA/PROLIFERATING CELL FACTOR1 (TCP) act as developmental regulators that have many roles in the growth and development processes throughout the entire life span of plants. TCP transcription factors are responsive to endogenous and environmental signals, such as salt stress. However, studies on the role of the TCP genes in salt stress response have rarely focused on woody plants, especially forest trees. In this study, the BpTCP3 gene, a CYC/TB1 subfamily member, isolated from Betula platyphylla Sukaczev, was significantly influenced by salt stress. The β-glucuronidase (GUS) staining analysis of transgenic B. platyphylla harboring the BpTCP3 promoter fused to the reporter gene GUS (pBpTCP3::GUS) further confirmed that the BpTCP3 gene acts a positive regulatory position in salt stress. Under salt stress, we found that the BpTCP3 overexpressed lines had increased relative/absolute high growth but decreased salt damage index, hydrogen peroxide (H2O2), and malondialdehyde (MDA) levels versus wild-type (WT) plants. Conversely, the BpTCP3 suppressed lines exhibited sensitivity to salt stress. These results indicate that the BpTCP3 transcription factor improves the salt tolerance of B. platyphylla by reducing reactive oxygen species damage, which provides useful clues for the functions of the CYC/TB1 subfamily gene in the salt stress response of B. platyphylla.

scholarly journals Improved salt tolerance in transgenic tobacco by over-expression of poplar NAC13 gene

2019 ◽  
Author(s):  
Xuemei Zhang ◽  
Zihan Cheng ◽  
Kai Zhao ◽  
Renhua Li ◽  
Boru Zhou ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Transgenic Tobacco ◽  
Stress Responses ◽  
Gene Networks ◽  
Molecular Mechanisms ◽  
Physiological Characterization ◽  
Protein Functions ◽  
Forest Genetic

Background: NACs are one of the major transcription factor families in plants which play an important role in plant growth and development, as well as in adverse stress responses. Methods: In this study, we cloned a salt-inducible NAC transcription factor gene (NAC13) from a poplar variety 84K, followed by transforming it into both tobacco and Arabidopsis. Results: Stable expression analysis of 35S::NAC13-GFP fusion protein in Arabidopsis indicated that NAC13 was localized to the nucleus. We also obtained five transgenic tobacco lines. Evidence from morphological and physiological characterization and salt treatment analyses indicated that the transgenic tobacco enhanced salt tolerance, suggesting that NAC13 gene may function as a positive regulator in tobacco responses to salt stress. Furthermore, evidence from yeast two-hybrid screening demonstrated that NAC13 protein functions as a transcriptional activator, with an activation domain located in the C-terminal region. Discussion: NAC13 gene plays an important role in response to salt stress in tobacco. Future studies are needed to shed light on molecular mechanisms of gene regulation and gene networks related to NAC13 gene in response to salt stress, which will provide a valuable theoretical basis for forest genetic breeding and resistant breeding.

Tobacco transcription factor bHLH123 improves salt tolerance by activating NADPH oxidase NtRbohE expression

2021 ◽  
Author(s):  
Dan Liu ◽  
Yang-Yang Li ◽  
Zhi-Cheng Zhou ◽  
Xiaohua Xiang ◽  
Xin Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Signaling Pathway ◽  
Stress Responses ◽  
Critical Role ◽  
Bhlh Transcription Factor ◽  
Ros Scavenging ◽  
Stress Related Genes ◽  
Respiratory Burst Oxidase

ABSTRACT In plants, reactive oxygen species (ROS) produced following the expression of the respiratory burst oxidase homolog (Rboh) gene are important regulators of stress responses. However, little is known about how plants acclimate to salt stress through the Rboh-derived ROS signaling pathway. Here, we showed that a 400-bp fragment of the tobacco (Nicotiana tabacum) NtRbohE promoter played a critical role in the salt response. Using yeast one-hybrid (Y1H) screens, NtbHLH123, a bHLH transcription factor, was identified as an upstream partner of the NtRbohE promoter. These interactions were confirmed by Y1H, electrophoretic mobility assay, and chromatin immunoprecipitation assays. Overexpression of NtbHLH123 resulted in greater resistance to salt stress, while NtbHLH123-silenced plants had reduced resistance to salt stress. We also found that NtbHLH123 positively regulates the expression of NtRbohE and ROS production soon after salt stress treatment. Moreover, knockout of NtRbohE in the 35S::NtbHLH123 background resulted in reduced expression of ROS-scavenging and salt stress-related genes and salt tolerance, suggesting that NtbHLH123-regulated salt tolerance is dependent on the NtbHLH123-NtRbohE signaling pathway. Our data show that NtbHLH123 is a positive regulator and acts as a molecular switch to control a Rboh-dependent mechanism in response to salt stress in plants.

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