Preparation of anisotropic multiscale micro-hydrogels via two-photon continuous flow lithography

2022 ◽  
Vol 608 ◽  
pp. 622-633
Author(s):  
Purnima N. Manghnani ◽  
Valentina Di Francesco ◽  
Carlo Panella La Capria ◽  
Michele Schlich ◽  
Marco Elvino Miali ◽  
...  
Keyword(s):  
2012 ◽  
Vol 24 (10) ◽  
pp. 1303-1303
Author(s):  
Simona C. Laza ◽  
Marco Polo ◽  
Antonio A. R. Neves ◽  
Roberto Cingolani ◽  
Andrea Camposeo ◽  
...  
Keyword(s):  

2012 ◽  
Vol 24 (10) ◽  
pp. 1304-1308 ◽  
Author(s):  
Simona C. Laza ◽  
Marco Polo ◽  
Antonio A. R. Neves ◽  
Roberto Cingolani ◽  
Andrea Camposeo ◽  
...  
Keyword(s):  

2020 ◽  
Vol 28 (26) ◽  
pp. 40088
Author(s):  
Samira Chizari ◽  
Shreya Udani ◽  
Amin Farzaneh ◽  
Daniel Stoecklein ◽  
Dino Di Carlo ◽  
...  
Keyword(s):  

2018 ◽  
Vol 26 (11) ◽  
pp. 14718 ◽  
Author(s):  
Lucas A. Shaw ◽  
Samira Chizari ◽  
Maxim Shusteff ◽  
Hamed Naghsh-Nilchi ◽  
Dino Di Carlo ◽  
...  

2018 ◽  
Vol 26 (10) ◽  
pp. 13543 ◽  
Author(s):  
Lucas A. Shaw ◽  
Samira Chizari ◽  
Maxim Shusteff ◽  
Hamed Naghsh-Nilchi ◽  
Dino Di Carlo ◽  
...  

Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.


Author(s):  
David W. Piston

Two-photon excitation fluorescence microscopy provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In our fluorescence experiments, the final excited state is the same singlet state that is populated during a conventional fluorescence experiment. Thus, the fluorophore exhibits the same emission properties (e.g. wavelength shifts, environmental sensitivity) used in typical biological microscopy studies. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10−5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.


2020 ◽  
Vol 22 (19) ◽  
pp. 6437-6443
Author(s):  
Cheng-Kou Liu ◽  
Meng-Yi Chen ◽  
Xin-Xin Lin ◽  
Zheng Fang ◽  
Kai Guo

A catalyst-, oxidant-, acidic solvent- and quaternary ammonium salt-free electrochemical para-selective hydroxylation of N-arylamides at rt in batch and continuous-flow was developed.


1996 ◽  
Vol 43 (9) ◽  
pp. 1765-1771 ◽  
Author(s):  
M. W. HAMILTON and D. S. ELLIOTT

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