scholarly journals First events in the coil-to-globule transition of PVME in water: an ultrafast temperature jump – time-resolved elastic light scattering study

Author(s):  
Marcin Pastorczak ◽  
Michał Nejbauer ◽  
Naoki Shinyashiki ◽  
Masanobu Takatsuka ◽  
Gonzalo Angulo ◽  
...  
2016 ◽  
Vol 113 (40) ◽  
pp. 11152-11157 ◽  
Author(s):  
Carlos G. Lopez ◽  
Oliva Saldanha ◽  
Klaus Huber ◽  
Sarah Köster

Vimentin intermediate filaments (IFs) are part of a family of proteins that constitute one of the three filament systems in the cytoskeleton, a major contributor to cell mechanics. One property that distinguishes IFs from the other cytoskeletal filament types, actin filaments and microtubules, is their highly hierarchical assembly pathway, where a lateral association step is followed by elongation. Here we present an innovative technique to follow the elongation reaction in solution and in situ by time-resolved static and dynamic light scattering, thereby precisely capturing the relevant time and length scales of seconds to minutes and 60–600 nm, respectively. We apply a quantitative model to our data and succeed in consistently describing the entire set of data, including particle mass, radius of gyration, and hydrodynamic radius during longitudinal association.


Polymer ◽  
1998 ◽  
Vol 39 (13) ◽  
pp. 2769-2775 ◽  
Author(s):  
Tomohisa Norisuye ◽  
Mitsuhiro Shibayama ◽  
Shunji Nomura

Langmuir ◽  
2010 ◽  
Vol 26 (14) ◽  
pp. 11605-11608 ◽  
Author(s):  
Hiroyuki Mori ◽  
Shoichi Kutsumizu ◽  
Kazuya Saito ◽  
Katsuhiro Yamamoto ◽  
Shinichi Sakurai ◽  
...  

2003 ◽  
Vol 107 (38) ◽  
pp. 10329-10332 ◽  
Author(s):  
Jun Kubota ◽  
Eisuke Yoda ◽  
Naoko Ishizawa ◽  
Akihide Wada ◽  
Kazunari Domen ◽  
...  

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