scholarly journals The changing face of SDS denaturation: Complexes of Thermomyces lanuginosus lipase with SDS at pH 4.0, 6.0 and 8.0

Author(s):  
Helena Østergaard Rasmussen ◽  
Daniel T. Weltz Wollenberg ◽  
Huabing Wang ◽  
Kell K. Andersen ◽  
Cristiano L. P. Oliveira ◽  
...  
2015 ◽  
Vol 1854 (12) ◽  
pp. 1914-1921 ◽  
Author(s):  
Jens Kvist Madsen ◽  
Thomas Rebsdorf Sørensen ◽  
Jørn Døvling Kaspersen ◽  
Maria Berggård Silow ◽  
Jesper Vind ◽  
...  

Catalysts ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 281 ◽  
Author(s):  
Anna Chojnacka ◽  
Witold Gładkowski

Synthesis of structured phosphatidylcholine (PC) enriched with myristic acid (MA) was conducted by acidolysis and interesterification reactions using immobilized lipases as catalysts and two acyl donors: trimyristin (TMA) isolated from ground nutmeg, and myristic acid obtained by saponification of TMA. Screening experiments indicated that the most effective biocatalyst for interesterification was Rhizomucor miehei lipase (RML), whereas for acidolysis, the most active were Thermomyces lanuginosus lipase (TLL) and RML. The effect of the molar ratio of substrates (egg-yolk PC/acyl donor), enzyme loading, and different solvent on the incorporation of MA into PC and on PC recovery was studied. The maximal incorporation of MA (44 wt%) was achieved after 48 h of RML-catalyzed interesterification in hexane using substrates molar ratio (PC/trimyristin) 1/5 and 30% enzyme load. Comparable results were obtained in toluene with 1/3 substrates molar ratio. Interesterification of PC with trimyristin resulted in significantly higher MA incorporation than acidolysis with myristic acid, particularly in the reactions catalyzed by RML.


2018 ◽  
Vol 38 (2) ◽  
pp. 608-613 ◽  
Author(s):  
Adriana Dantas ◽  
Alexsandra Valério ◽  
Jorge Luiz Ninow ◽  
J. Vladimir de Oliveira ◽  
Débora de Oliveira

2020 ◽  
Vol 24 ◽  
pp. 101197
Author(s):  
Jing Li ◽  
Jiandong Zhang ◽  
Shuguang Shen ◽  
Bing Zhang ◽  
William W. Yu

2017 ◽  
Vol 149 ◽  
pp. 280-287 ◽  
Author(s):  
Marco Muth ◽  
Stefanie Rothkötter ◽  
Steven Paprosch ◽  
Reiner P. Schmid ◽  
Klaus Schnitzlein

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