Philippine Pili: Composition of the lipid molecular species

2015 ◽  
Vol 2 (4) ◽  
pp. 147-153 ◽  
Laura J. Pham ◽  
Nico G. Dumandan
1974 ◽  
Vol 38 (7) ◽  
pp. 1371-1376 ◽  
Tetsuya Suzuki ◽  
Kiyozo Hasegawa

2000 ◽  
Vol 352 (1) ◽  
pp. 79 ◽  
Xianlin HAN ◽  
John G. KELLEY ◽  
Richard W. GROSS

1998 ◽  
Vol 47 (7) ◽  
pp. 1195-1200 ◽  
Fatiha Aid ◽  
Ghouziel Kesri-Benhassaine ◽  
Chantal Demandre ◽  
Paul Mazliak

1999 ◽  
Vol 146 (4) ◽  
pp. 741-754 ◽  
Roger Schneiter ◽  
Britta Brügger ◽  
Roger Sandhoff ◽  
Günther Zellnig ◽  
Andrea Leber ◽  

Nano-electrospray ionization tandem mass spectrometry (nano-ESI-MS/MS) was employed to determine qualitative differences in the lipid molecular species composition of a comprehensive set of organellar membranes, isolated from a single culture of Saccharomyces cerevisiae cells. Remarkable differences in the acyl chain composition of biosynthetically related phospholipid classes were observed. Acyl chain saturation was lowest in phosphatidylcholine (15.4%) and phosphatidylethanolamine (PE; 16.2%), followed by phosphatidylserine (PS; 29.4%), and highest in phosphatidylinositol (53.1%). The lipid molecular species profiles of the various membranes were generally similar, with a deviation from a calculated average profile of ∼± 20%. Nevertheless, clear distinctions between the molecular species profiles of different membranes were observed, suggesting that lipid sorting mechanisms are operating at the level of individual molecular species to maintain the specific lipid composition of a given membrane. Most notably, the plasma membrane is enriched in saturated species of PS and PE. The nature of the sorting mechanism that determines the lipid composition of the plasma membrane was investigated further. The accumulation of monounsaturated species of PS at the expense of diunsaturated species in the plasma membrane of wild-type cells was reversed in elo3Δ mutant cells, which synthesize C24 fatty acid-substituted sphingolipids instead of the normal C26 fatty acid-substituted species. This observation suggests that acyl chain-based sorting and/or remodeling mechanisms are operating to maintain the specific lipid molecular species composition of the yeast plasma membrane.

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