Assembly of functional microbial communities in paddy soil with long-term application of pig manure under rice-rape cropping system

2022 ◽  
Vol 305 ◽  
pp. 114374
Author(s):  
Qiong Hou ◽  
Sen Lin ◽  
Yuemin Ni ◽  
Longren Yao ◽  
Shan Huang ◽  
...  
2015 ◽  
Vol 61 (3-4) ◽  
pp. 146-156 ◽  
Author(s):  
Yun Wu ◽  
Chuan-Hai Li ◽  
Juan Zhao ◽  
Yong-Liang Xiao ◽  
Hui Cao

The soil microbial community research conducted in the field has focused on the genetic diversity of these organisms. In this study, we assessed the proteins expressed in soil microbial communities following the long-term application of mineral fertilizer (NPK) and organic manure (M) to paddy soil, indirect extraction method and separated via two-dimensional (2D) gel electrophoresis and identified using a matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) approach. We found that the number of cells in the primary soil in the M treatment was significantly greater than in the NPK and CK treatments. The numbers of cells extracted were consistent with the total cell numbers and the concentration of extracted proteins (CK < NPK < M). 303 and 306 protein spots being detected in the CK map and NPK map, respectively. Eleven spots of interest were identified in the 2D gels, including 8 different protein spots and 3 unique protein spots. Three common proteins involved in protein hydrolysis and glutamate synthesis and metabolism. Eight differentially expressed proteins involved in DNA replication, transcription, protein folding and energy metabolism, the processes of cofactor and vitamin metabolism, transcriptional regulation, recombination and xenobiotic compound biodegradation and metabolism. The long-term application of fertilization resulted in significant changes in the microbial community structure and function, and the long-term application of pig manure significantly increased the microbial biomass and the functional and structural diversity in the soil. It is very interesting to address the MS identification of intracellular proteins from microbial communities under different fertilizer treatments in a paddy soil.


2020 ◽  
Vol 11 ◽  
Author(s):  
Ali Y. Srour ◽  
Hala A. Ammar ◽  
Arjun Subedi ◽  
Mirian Pimentel ◽  
Rachel L. Cook ◽  
...  

2015 ◽  
Vol 14 (12) ◽  
pp. 2512-2520 ◽  
Author(s):  
Song-juan GAO ◽  
Ren-gang ZHANG ◽  
Wei-dong CAO ◽  
Yuan-yuan FAN ◽  
Ju-sheng GAO ◽  
...  

Author(s):  
Jessica Cuartero ◽  
Onurcan Özbolat ◽  
Virginia Sánchez-Navarro ◽  
Marcos Egea-Cortines ◽  
Raúl Zornoza ◽  
...  

Microbial communities play a key role in sustainable agriculture. However, we still need more in-formation, to understand the complex response of the microbial community to long-term organic farming, which aims to reduce synthetic fertilizer and pesticide use in order to produce sustainably and improve soil quality. We have assessed the long-term effect of two organic cropping systems and a conventional system on the microbial soil community structure using high-throughput se-quencing analysis. We analyzed the link between these communities and changes in soil properties and crop yield. Results showed that the crop yield was similar among the three cropping systems. Soil properties, such as total organic carbon, nitrogen, ammonium, magnesium and boron, influ-enced changes in the bacterial community structure. A linear discriminant analysis effect size (LEfSe) showed different bacteria and fungi as key microorganism of each of the three different cropping systems, in addition, our results reflected that fungal community were more sensitive than bacteria to cropping system. This research provides an insight about changes occurred in soils, especially in microbial communities considering the effect of that changes in crop yield which were remained stable among the different cropping systems.


2012 ◽  
Vol 11 (4) ◽  
pp. 655-664 ◽  
Author(s):  
Zeng-ping YANG ◽  
Ming-gang XU ◽  
Sheng-xian ZHENG ◽  
Jun NIE ◽  
Ju-sheng GAO ◽  
...  

2021 ◽  
Vol 166 ◽  
pp. 104063
Author(s):  
Muhammad Ahmed Waqas ◽  
Yu’e Li ◽  
Muhammad Nadeem Ashraf ◽  
Waqas Ahmed ◽  
Bin Wang ◽  
...  

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