scholarly journals Nutritionally induced nanoscale variations in spider silk structural and mechanical properties

Author(s):  
Sean J. Blamires ◽  
Madeleine Nobbs ◽  
Jonas O. Wolff ◽  
Celine Heu
Author(s):  
Margret Weissbach ◽  
Marius Neugebauer ◽  
Anna-Christin Joel

AbstractSpider silk attracts researchers from the most diverse fields, such as material science or medicine. However, still little is known about silk aside from its molecular structure and material strength. Spiders produce many different silks and even join several silk types to one functional unit. In cribellate spiders, a complex multi-fibre system with up to six different silks affects the adherence to the prey. The assembly of these cribellate capture threads influences the mechanical properties as each fibre type absorbs forces specifically. For the interplay of fibres, spinnerets have to move spatially and come into contact with each other at specific points in time. However, spinneret kinematics are not well described though highly sophisticated movements are performed which are in no way inferior to the movements of other flexible appendages. We describe here the kinematics for the spinnerets involved in the cribellate spinning process of the grey house spider, Badumna longinqua, as an example of spinneret kinematics in general. With this information, we set a basis for understanding spinneret kinematics in other spinning processes of spiders and additionally provide inspiration for biomimetic multiple fibre spinning.


2005 ◽  
Vol 4 (12) ◽  
pp. 901-905 ◽  
Author(s):  
Yi Liu ◽  
Zhengzhong Shao ◽  
Fritz Vollrath

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 511
Author(s):  
Yu Suzuki ◽  
Takanori Higashi ◽  
Takahiro Yamamoto ◽  
Hideyasu Okamura ◽  
Takehiro K. Sato ◽  
...  

Spider dragline silk is a biopolymer with excellent mechanical properties. The development of recombinant spider silk protein (RSP)-based materials with these properties is desirable. Formic acid (FA) is a spinning solvent for regenerated Bombyx mori silk fiber with excellent mechanical properties. To use FA as a spinning solvent for RSP with the sequence of major ampullate spider silk protein from Araneus diadematus, we determined the conformation of RSP in FA using solution NMR to determine the role of FA as a spinning solvent. We assigned 1H, 13C, and 15N chemical shifts to 32-residue repetitive sequences, including polyAla and Gly-rich regions of RSP. Chemical shift evaluation revealed that RSP is in mainly random coil conformation with partially type II β-turn structure in the Gly-Pro-Gly-X motifs of the Gly-rich region in FA, which was confirmed by the 15N NOE data. In addition, formylation at the Ser OH groups occurred in FA. Furthermore, we evaluated the conformation of the as-cast film of RSP dissolved in FA using solid-state NMR and found that β-sheet structure was predominantly formed.


2013 ◽  
Vol 69 (9) ◽  
pp. P_286-P_292
Author(s):  
MARI INOUE

Polymer ◽  
1999 ◽  
Vol 40 (7) ◽  
pp. 1799-1806 ◽  
Author(s):  
Zhengzhong Shao ◽  
Fritz Vollrath

2011 ◽  
Vol 21 (35) ◽  
pp. 13594 ◽  
Author(s):  
Kristina Spiess ◽  
Roxana Ene ◽  
Caroline D. Keenan ◽  
Jürgen Senker ◽  
Friedrich Kremer ◽  
...  

2012 ◽  
Vol 13 (8) ◽  
pp. 2240-2246 ◽  
Author(s):  
Matthew Hudspeth ◽  
Xu Nie ◽  
Weinong Chen ◽  
Randolph Lewis

2018 ◽  
Vol 19 (9) ◽  
pp. 3853-3860 ◽  
Author(s):  
Christopher H. Bowen ◽  
Bin Dai ◽  
Cameron J. Sargent ◽  
Wenqin Bai ◽  
Pranay Ladiwala ◽  
...  

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