Impact of sodium hypochlorite on organic tissue dissolution in the periapical region of immature permanent teeth: an ex vivo study

2022 ◽  
Author(s):  
José Ricardo Claudino Ribeiro ◽  
Carlos Eduardo da Silveira Bueno ◽  
Kely Firmino Bruno ◽  
Samuel dos Reis ◽  
Alexandre Sigrist de Martin ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Ex Vivo ◽  
Permanent Teeth ◽  
Organic Tissue ◽  
Ex Vivo Study

scholarly journals Root Canal Penetration of a Sodium Hypochlorite Mixture Using Sonic or Ultrasonic Activation

2014 ◽  
Vol 25 (6) ◽  
pp. 489-493 ◽  
Author(s):  
Marta Sáinz-Pardo ◽  
Roberto Estevez ◽  
Óliver Valencia de Pablo ◽  
Giampiero Rossi-Fedele ◽  
Rafael Cisneros
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Ex Vivo ◽  
Positive Pressure ◽  
Ultrasonic Activation ◽  
Penetration Length ◽  
Working Length ◽  
Closed Systems ◽  
Canal Shaping ◽  
Ex Vivo Study

The purpose of this ex vivo study was to determine, in "open" and "closed" systems, whether the design has an influence on the penetration length of sodium hypochlorite mixed with a radiopaque contrast medium, measured in millimeters, when delivered using positive pressure (PP) and using sonic (SI) or passive ultrasonic (PUI) activation. Sixty single-rooted teeth were divided into two groups: open and closed systems (n=30). Root canal shaping was performed to a working length of 17 mm. The samples were divided into three sub-groups (n=10) according to irrigant delivery and activation: PP, and SI or PUI activation. By using radiographs, penetration length was measured, and vapor lock was assessed. For the closed group, the penetration distance means were: PP 15.715 (±0.898) mm, SI 16.299 (±0.738) mm and PUI 16.813 (±0.465) mm, with vapor lock occurring in 53.3% of the specimens. In the open group, penetration to 17 mm occurred in 97.6% of the samples, and no vapor lock occurred. Irrigant penetration and distribution evaluation using open and closed systems provide significantly different results. For closed systems, PUI is the most effective in delivering the irrigant to working length, followed by SI.

Can the sodium hypochlorite tissue dissolution ability during endodontic treatment really be trusted? An in vitro and ex vivo study

2013 ◽  
Vol 3 (2) ◽  
pp. 24-29
Author(s):  
Alexandre Augusto Zaia ◽  
Emmanuel João Nogueira Leal Silva ◽  
Aline Cristine Gomes ◽  
Danna Mota Moreira
Keyword(s):  
Sodium Hypochlorite ◽  
Ex Vivo ◽  
Endodontic Treatment ◽  
Ex Vivo Study

Physical Effects of Sodium Hypochlorite on Bone: An Ex Vivo Study

2012 ◽  
Vol 38 (3) ◽  
pp. 357-359 ◽  
Author(s):  
Frank M. Kerbl ◽  
Patricia DeVilliers ◽  
Mark Litaker ◽  
Paul D. Eleazer
Keyword(s):  
Sodium Hypochlorite ◽  
Ex Vivo ◽  
Physical Effects ◽  
Ex Vivo Study

scholarly journals Effect of Different Instrumentation and Irrigation Methods on Apical Microbial Extrusion: An Ex Vivo Study

2021 ◽  
Vol 14 (4) ◽  
pp. 2173-2182
Author(s):  
Sachin Metkari ◽  
Dinesh Rao ◽  
Kulvindersingh Banga
Keyword(s):  
Ex Vivo ◽  
Irrigation System ◽  
Positive Control ◽  
Permanent Teeth ◽  
Contributing Factors ◽  
Irrigation Methods ◽  
Colony Forming Units ◽  
Group 3 ◽  
Group 2 ◽  
Ex Vivo Study

Background: Periapical extrusion of debris, irrigating solution and microorganism are the major contributing factors for flare-ups during root canal therapy. The aim of this ex vivo study was to evaluate the effect of different types of instrumentation in combination with different irrigation methods on apical bacterial expulsion. Material and Methods: Three hundred and ten extracted human permanent teeth were infected with Enterococcus faecalis. After incubation at 37°C for 24 h, three hundred teeth were instrumented with three different instrumentations using two irrigation methods. The remaining ten teeth were used as negative and positive control groups, in which no inoculation was done and no instrumentation was carried out respectively. Three hundred teeth were equally divided in three groups (n = 100), in which instrumentation was performed using a protaper universal rotary file (group 1), WaveOne reciprocating file (group 2) and a flexiCON rotary file (group 3). In each group, 50 samples were irrigated with conventional needle irrigation, and 50 samples were irrigated with the endoVac irrigation method. During instrumentation, apically extruded bacteria were collected in an Eppendorf tube. Microbiological samples were taken from the Eppendorf tube and incubated for 24h, and colony-forming units were counted. The data collected were statistically analysed. Results: The group 2 showed highest bacterial extrusion using conventional irrigation while group 3 showed lowest using endovac irrigation system. Conclusion: FlexiCON rotary instrumentation with the endoVac irrigation system produced significantly less bacterial extrusion than the other techniques.

scholarly journals Evaluation of the effect of irrigants on the accuracy and repeatability of three electronic apex locators: an ex vivo study

2021 ◽  
Vol 10 (13) ◽  
pp. e599101321636
Author(s):  
Fábio Luiz Cunha D'Assunção ◽  
Juan Ramon Salazar Silva ◽  
Karen Christine Lacerda Arnaud ◽  
Thiago Bezerra Leite ◽  
Jozemar Pereira dos Santos ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Intraclass Correlation Coefficient ◽  
Sodium Hypochlorite ◽  
Ex Vivo ◽  
Intraclass Correlation ◽  
Repeatability Coefficient ◽  
Human Teeth ◽  
Apical Constriction ◽  
Accuracy And Repeatability ◽  
Ex Vivo Study

Objective: To evaluate the effect of 2% chlorhexidine (CHX) and 2.5% sodium hypochlorite (NaOCl) on the accuracy and repeatability of three Electronic Apex Locators (EALs). Methodology: Thirty one human teeth were connected to a platform, irrigated with 2.0% CHX, and obtained two readings, again irrigated with 2.5% NaOCl and obtained two more readings. The distance between the tip of the file and the apical constriction was measured. Repeatability was calculated using the intraclass correlation coefficient (ICC) and repeatability coefficient (CR). Results: For CHX 2%, with the Mini Apex Locator, 25 of 31 electronic measurements were accurate (80.6%), 28 of 31 measurements for iPex (90.3%) and for Root ZX II 26 (83, 9%). For 2.5% NaOCl, with the Mini Apex Locator, 25 of the 31 measurements were accurate (80.6%), 28 of the 31 measurements for the iPex (90.3%) and for the Root ZX II, 24 (77, 4%). There were no differences between the two irrigants (p>0.05). The values ​​(ICC) were 0.975 and 0.994 for the Mini Apex Locator, 0.981 and 0.971 for the i Pex and 0.994 and 0.995 for the Root ZX II. When using CHX, the (CR) was 0.196 for the Mini Apex Locator, 0.152 for the iPex and 0.088 for the Root ZX II. When using 2.5% NaOCl, the (CR) was 0.088 for the Mini Apex Locator, 0.196 for the iPex and 0.088 for the Root ZX II. Conclusions: The use of 2% CHX and 2.5% NaOCl did not affect the accuracy and repeatability of the three devices.

scholarly journals Root canal debridement efficacy of heated sodium hypochlorite in conjunction with passive ultrasonic agitation: An ex vivo study

2020 ◽  
Vol 14 (4) ◽  
pp. 235-238
Author(s):  
Yogesh Damade ◽  
Ramchandra Kabir ◽  
Sunanda Gaddalay ◽  
Sharvaree Deshpande ◽  
Sonali Gite ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Ex Vivo ◽  
Canal System ◽  
Ultrasonic Agitation ◽  
Root Canal System ◽  
Post Hoc ◽  
Root Canal Instrumentation ◽  
Ex Vivo Study

Background. This study aimed to investigate the endodontic debridement efficacy of different sodium hypochlorite (NaOCl) irrigation regimens with and without ultrasonic agitation, followed by ethylenediaminetetraacetic acid (EDTA) via scanning electron microscopy (SEM) after using a rotary instrumentation system. Methods. Mandibular premolars (n=50) were randomly divided into five experimental groups (n=10) for root canal instrumentation with ProTaper Universal rotary system up to F3. The root canal system was treated with intracanal-heated NaOCl (100°C) or preheated NaOCl (55°C), followed by ultrasonic agitation and EDTA treatment. Samples irrigated with conventional needle irrigation (CNI) using normal saline solution were used as controls. Debridement efficacy was analyzed by SEM. A five-point scale was used to estimate the presence/absence of debris for each canal segment (coronal, middle, and apical). The results were analyzed using one-way ANOVA and post hoc Tukey tests (P<0.05). Results. The experimental groups exhibited less debris compared to CNI with saline (P<0.05). The amount of debris decreased significantly for the group with NaOCl intracanal heating compared to extraoral heating. Ultrasonic agitation further enhanced the root canal debridement efficacy of NaOCl. Conclusion. In summary, intracanal heating of NaOCl with and without ultrasonic agitation followed by EDTA appears to be a promising method to flush debris from the root canal system.

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