Corrigendum to “Differentiation of Adipose-Derived Stem Cells Promotes Regeneration of Smooth Muscle for Ureteral Tissue Engineering” [Journal of Surgical Research 178 (2012) 55-62]

Tissue engineering has shown great promise in generating vascular grafts with properties similar to that of native blood vessels. Vascular Smooth Muscle Cells (VSMC) are the main component of the vasculature tunica media. Recreation of this layer represents a major challenge in tissue engineering due to difficulties in harvesting and culturing autologous VSMC. The use of stem cells and their inherent ability to differentiate into diverse cell types, including vascular lineages, have been proposed

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Differentiation of Adipose-Derived Stem Cells into Vascular Smooth Muscle Cells for Tissue Engineering Applications

Biomedicines ◽

10.3390/biomedicines9070797 ◽

2021 ◽

Vol 9 (7) ◽

pp. 797

Author(s):

Alvaro Yogi ◽

Marina Rukhlova ◽

Claudie Charlebois ◽

Ganghong Tian ◽

Danica B. Stanimirovic ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Smooth Muscle ◽

Smooth Muscle Cells ◽

Neointimal Hyperplasia ◽

Muscle Cells ◽

Regular Growth ◽

Growth Media ◽

Adipose Derived Stem Cells ◽

Vascular Bypass

Synthetic grafts have been developed for vascular bypass surgery, however, the risks of thrombosis and neointimal hyperplasia still limit their use. Tissue engineering with the use of adipose-derived stem cells (ASCs) has shown promise in addressing these limitations. Here we further characterized and optimized the ASC differentiation into smooth muscle cells (VSMCs) induced by TGF-β and BMP-4. TGF-β and BMP-4 induced a time-dependent expression of SMC markers in ASC. Shortening the differentiation period from 7 to 4 days did not impair the functional property of contraction in these cells. Stability of the process was demonstrated by switching cells to regular growth media for up to 14 days. The role of IGFBP7, a downstream effector of TGF-β, was also examined. Finally, topographic and surface patterning of a substrate is recognized as a powerful tool for regulating cell differentiation. Here we provide evidence that a non-woven PET structure does not affect the differentiation of ASC. Taken together, our results indicate that VSMCs differentiated from ASCs are a suitable candidate to populate a PET-based vascular scaffolds. By employing an autologous source of cells we provide a novel alternative to address major issues that reduces long-term patency of currently vascular grafts.

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Differentiation of adipose-derived stem cells promotes regeneration of smooth muscle for ureteral tissue engineering

Journal of Surgical Research ◽

10.1016/j.jss.2012.01.047 ◽

2012 ◽

Vol 178 (1) ◽

pp. 55-62 ◽

Cited By ~ 36

Author(s):

Zhankui Zhao ◽

Honglian Yu ◽

Fei Xiao ◽

Xinghuan Wang ◽

Sixing Yang ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Smooth Muscle ◽

Adipose Derived Stem Cells

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The role of adipose derived stem cells, smooth muscle cells and low intensity laser irradiation (LILI) in tissue engineering and regenerative medicine

Open Life Sciences ◽

10.2478/s11535-013-0145-x ◽

2013 ◽

Vol 8 (4) ◽

pp. 331-336

Author(s):

Bernard Mvula ◽

Heidi Abrahamse

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Smooth Muscle ◽

Regenerative Medicine ◽

Smooth Muscle Cells ◽

Muscle Cells ◽

Adipose Derived Stem Cells ◽

Low Intensity ◽

Intensity Laser

AbstractTissue engineering and regenerative medicine has become the treatment of choice for several degenerative diseases. It involves the repairing or replacing of diseased or damaged cells or tissues. Stem cells have a key role to play in this multidisciplinary science because of their capacity to differentiate into several lineages. Adipose derived stem cells (ADSCs) are adult mesenchymal stem cells that are easily harvested and have the capacity to differentiate into cartilage, bone, smooth muscle, fat, liver and nerve cells. ADSCs have been found to differentiate into smooth muscle cells which play major roles in diseases such as asthma, hypertension, cancer and arteriosclerosis. Low Intensity Laser Irradiation (LILI), which involves the application of monochromatic light, has been found to increase viability, proliferation and differentiation in several types of cells including ADSCs. This review discusses the role of ADSCs, smooth muscle cells and LILI in the science of tissue engineering and regenerative medicine.

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In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

Revista de Chimie ◽

10.37358/rc.17.6.5670 ◽

2017 ◽

Vol 68 (6) ◽

pp. 1341-1344

Author(s):

Grigore Berea ◽

Gheorghe Gh. Balan ◽

Vasile Sandru ◽

Paul Dan Sirbu

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Endothelial Cells ◽

Single Cell ◽

Cell Line ◽

Bone Repair ◽

Umbilical Vein ◽

Human Fibroblasts ◽

Three Dimensional ◽

Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

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