Comparison of two highly sensitive methods to detect potato spindle tuber viroid in Dahlia using quantitative-reverse transcription-polymerase chain reaction assays

2022 ◽  
Vol 300 ◽  
pp. 114401
Author(s):  
Daiki Tsushima ◽  
Shin-ichi Fuji
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Potato Spindle Tuber Viroid ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain

scholarly journals Detection of prostate-specific antigen mRNA and protein in breast tumors

1996 ◽  
Vol 42 (3) ◽  
pp. 361-366 ◽  
Author(s):  
N Zarghami ◽  
E P Diamandis
Keyword(s):  
Polymerase Chain Reaction ◽  
Prostate Specific Antigen ◽  
Reverse Transcription ◽  
Specific Antigen ◽  
Breast Tumors ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Good Agreement

Abstract We have developed reverse transcription-polymerase chain reaction (RT- PCR) methods for detecting prostate-specific antigen (PSA) mRNA. Using these methods, and a highly sensitive immunofluorometric assay for measuring PSA protein, we have assessed the concentrations of PSA mRNA and PSA protein in 30 primary breast tumors and a few other control tissues. We found good agreement between presence of PSA protein and PSA mRNA in breast tumors. We thus propose that, in women, detection of PSA protein or PSA mRNA in tissues and tumors offers equivalent information. Because PSA protein is present in male blood and thus could contaminate extracts from tumors and tissues from men, we propose that the RT-PCR methods we describe be used to assess nonprostatic expression of the PSA gene in men.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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