Galangin alleviates vascular dysfunction and remodelling through modulation of the TNF-R1, p-NF-κB and VCAM-1 pathways in hypertensive rats

Life Sciences ◽  
2021 ◽  
pp. 119965
Author(s):  
Nisita Chaihongsa ◽  
Putcharawipa Maneesai ◽  
Weerapon Sangartit ◽  
Prapassorn Potue ◽  
Sarawoot Bunbupha ◽  
...  
2009 ◽  
Vol 118 (4) ◽  
pp. 291-301 ◽  
Author(s):  
Ahmed A. Elmarakby ◽  
John D. Imig

Obesity and hypertension are the two major risk factors that contribute to the progression of end-stage renal disease. To examine whether hypertension further exacerbates oxidative stress and vascular dysfunction and inflammation in obese rats, four groups of male Sprague–Dawley rats were fed either a normal (7% fat) or high-fat (36% fat) diet for 6 weeks and osmotic pumps were implanted to deliver ANG (angiotensin II) or vehicle for an additional 4 weeks. Treatment with the high-fat diet did not alter ANG-induced hypertension compared with the normal diet (174±6 compared with 170±5 mmHg respectively). Treatment with the high-fat diet increased body weight gain and plasma leptin levels and induced insulin resistance in normotensive and ANG-induced hypertensive rats. Plasma TBARS (thiobarbituric acid-reacting substances), a measure of oxidative stress, were elevated in high-fat diet-fed rats compared with controls (11.2±1 compared with 8.4±1 nmol/ml respectively) and was increased further in ANG-induced hypertensive rats fed a high-fat diet (18.8±2.2 nmol/ml). Urinary nitrite excretion was also decreased in rats fed a high-fat diet without or with ANG infusion compared with controls. Afferent arteriolar relaxation to acetylcholine was impaired in rats fed the high-fat diet without or with ANG infusion. Renal cortical TNF-α (tumour necrosis factor-α), COX-2 (cyclo-oxygenase-2) and phospho-IKK (inhibitor of nuclear factor κB kinase) expression increased in high-fat diet-fed rats compared with normal diet-fed rats. The increases in phospho-IKK and COX-2 expression were elevated further in ANG-induced hypertensive rats fed the high-fat diet. These results suggest that ANG-induced hypertension exacerbates oxidative stress and renal inflammation without further impairment in vascular dysfunction in high-fat diet-induced obesity.


2008 ◽  
Vol 591 (1-3) ◽  
pp. 224-230 ◽  
Author(s):  
Marcio L.L. Martinez ◽  
Michele M. Castro ◽  
Elen Rizzi ◽  
Karla Fernandes ◽  
Caroline Demacq ◽  
...  

1998 ◽  
Vol 274 (6) ◽  
pp. R1613-R1618 ◽  
Author(s):  
Ararat D. Giulumian ◽  
David M. Pollock ◽  
Natalie Clarke ◽  
Leslie C. Fuchs

Endothelin-1 (ET-1) is thought to play an important role in the development of deoxycorticosterone acetate (DOCA)-salt hypertension. Because hypertension is associated with an increased incidence of coronary artery disease, this study was designed to determine if coronary vascular contraction to ET-1 is altered in DOCA-salt hypertensive rats and to determine the effect of chronic treatment of DOCA-salt rats with the selective ETA receptor antagonist A-127722. Male Sprague-Dawley rats were divided into four groups: DOCA, Placebo, DOCA + A-127722, and Placebo + A-127722. A-127722 was administered in drinking water at a concentration of 8 mg/100 ml. After 3 wk, mean arterial pressure (MAP) was significantly enhanced in DOCA-salt compared with Placebo rats. A-127722 significantly inhibited the increase in MAP. Contraction to ET-1 (10−11 to 3 × 10−8 M) was measured in isolated coronary and mesenteric small arteries (200–300 μm, intraluminal diameter) maintained at a constant intraluminal pressure of 40 mmHg and was significantly impaired in vessels from DOCA-salt compared with Placebo rats. Dose-dependent contractions to KCl were also inhibited in coronary, but only minimally impaired in mesenteric, arteries of DOCA-salt rats. Inhibition of nitric oxide synthase activity did not restore contraction to ET-1 in coronary small arteries. However contractions to ET-1 were enhanced in mesenteric small arteries. Chronic treatment with A-127722 significantly restored contraction to ET-1 in coronary, but not in mesenteric, arteries of DOCA-salt rats. Because ETAreceptor blockade impairs the development of hypertension and improves coronary vascular reactivity, these data indicate that ET-1 plays an important role in coronary vascular dysfunction associated with DOCA-salt hypertension.


2000 ◽  
Vol 398 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Markus Lassila ◽  
Piet Finckenberg ◽  
Anna-Kaisa Pere ◽  
Heikki Vapaatalo ◽  
Marja-Leena Nurminen

2008 ◽  
Vol 198 (2) ◽  
pp. 320-331 ◽  
Author(s):  
Michele M. Castro ◽  
Elen Rizzi ◽  
Lívia Figueiredo-Lopes ◽  
Karla Fernandes ◽  
Lusiane M. Bendhack ◽  
...  

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