Glutamate mediates cell death and increases the Bax to Bcl-2 ratio in a differentiated neuronal cell line

2004 ◽  
Vol 128 (2) ◽  
pp. 160-169 ◽  
Author(s):  
William R. Schelman ◽  
Robert D. Andres ◽  
Kimberly J. Sipe ◽  
Evan Kang ◽  
James A. Weyhenmeyer
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Neuronal Cell ◽  
Neuronal Cell Line

scholarly journals Deletions in mitochondrial DNA (mt-DNA) during hypoxic cell death in the human NT2-N neuronal cell line

1997 ◽  
Vol 42 (3) ◽  
pp. 387-387
Author(s):  
Terje Rootwelt ◽  
Usha Reddy ◽  
Bernard Fromenty ◽  
David Pleasure
Keyword(s):  
Cell Death ◽  
Mitochondrial Dna ◽  
Cell Line ◽  
Neuronal Cell ◽  
Hypoxic Cell ◽  
Mt Dna ◽  
Neuronal Cell Line

scholarly journals Hypoxic cell death in the human NT2-N neuronal cell line

1997 ◽  
Vol 42 (3) ◽  
pp. 387-387
Author(s):  
Terje Rootwelt ◽  
Michelle Dunn ◽  
Marc Yudkoff ◽  
Takayuki Itoh ◽  
David Pleasure
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Neuronal Cell ◽  
Hypoxic Cell ◽  
Neuronal Cell Line

scholarly journals Rhes, a striatal-enriched protein, promotes mitophagy via Nix

2019 ◽  
Vol 116 (47) ◽  
pp. 23760-23771 ◽  
Author(s):  
Manish Sharma ◽  
Uri Nimrod Ramírez-Jarquín ◽  
Oscar Rivera ◽  
Melissa Kazantzis ◽  
Mehdi Eshraghi ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Neuronal Cell ◽  
Dependent Manner ◽  
Systemic Injection ◽  
Neuronal Cell Line ◽  
In The Wild ◽  
Striatal Lesion ◽  
The Brain

Elimination of dysfunctional mitochondria via mitophagy is essential for cell survival and neuronal functions. But, how impaired mitophagy participates in tissue-specific vulnerability in the brain remains unclear. Here, we find that striatal-enriched protein, Rhes, is a critical regulator of mitophagy and striatal vulnerability in brain. In vivo interactome and density fractionation reveal that Rhes coimmunoprecipitates and cosediments with mitochondrial and lysosomal proteins. Live-cell imaging of cultured striatal neuronal cell line shows Rhes surrounds globular mitochondria, recruits lysosomes, and ultimately degrades mitochondria. In the presence of 3-nitropropionic acid (3-NP), an inhibitor of succinate dehydrogenase, Rhes disrupts mitochondrial membrane potential (ΔΨm) and promotes excessive mitophagy and cell death. Ultrastructural analysis reveals that systemic injection of 3-NP in mice promotes globular mitochondria, accumulation of mitophagosomes, and striatal lesion only in the wild-type (WT), but not in the Rhes knockout (KO), striatum, suggesting that Rhes is critical for mitophagy and neuronal death in vivo. Mechanistically, Rhes requires Nix (BNIP3L), a known receptor of mitophagy, to disrupt ΔΨm and promote mitophagy and cell death. Rhes interacts with Nix via SUMO E3-ligase domain, and Nix depletion totally abrogates Rhes-mediated mitophagy and cell death in the cultured striatal neuronal cell line. Finally, we find that Rhes, which travels from cell to cell via tunneling nanotube (TNT)-like cellular protrusions, interacts with dysfunctional mitochondria in the neighboring cell in a Nix-dependent manner. Collectively, Rhes is a major regulator of mitophagy via Nix, which may determine striatal vulnerability in the brain.

ELEVATED CYTOSOLIC Ca2+DOES NOT MEDIATE CELL DEATH FROM CHEMICAL ANOXIA IN A NEURONAL CELL LINE

1991 ◽  
Vol 75 (3) ◽  
pp. A612-A612
Author(s):  
M. E. Johnson ◽  
C. B. Uhl ◽  
GJ. Gores ◽  
J. C. Sill
Keyword(s):  
Cell Death ◽  
Cell Line ◽  
Neuronal Cell ◽  
Neuronal Cell Line ◽  
Mediate Cell ◽  
Chemical Anoxia

scholarly journals Hypoxic cell death in the human NT2-N neuronal cell line. Involvement of NMDA and non-NMDA receptors 1758

1997 ◽  
Vol 41 ◽  
pp. 295-295
Author(s):  
Terje Rootwelt ◽  
Michelle Dunn ◽  
Marc Yudkoff ◽  
Takayuki Itoh ◽  
David Pleasure
Keyword(s):  
Cell Death ◽  
Nmda Receptors ◽  
Cell Line ◽  
Neuronal Cell ◽  
Hypoxic Cell ◽  
Neuronal Cell Line
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