Green synthesis of antimicrobial silver nanoparticles using fruit extract of Glycosmis pentaphylla and its theoretical explanations

Abstract The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this study for synthesis of silver nanoparticles different ratios of 1mM silver nitrate and Moringa oleifera leaf extract i.e, 95:5, 90:10 and 85: 15 was taken in conical flask and kept for one 1 hr at 25 0 c on magnetic stirrer, out of which 90:10 ratio was selected for further study based on highest peak, good size and stability. Tamarindus indica fruit extract was added to silver nitrate solution till the colour of the solution changes from light brown to chocolate brownish colour. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy, Zeta potential, size distribution by intensity. The absorption spectrum of the silver nano solution prepared by using Moringa oleifera and Tamarindus indica fruit extract showed a surface plasmon absorption band with maximum of 420 nm and 430 nm respectively indicating the presence of silver nanoparticles. The zeta value of silver nanoparticles synthesized from Moringa oleifera and Tamarindus indica fruit extract was -12.5 mV and -15.5 mV, size of 110.2 nm and 130.2 nm respectively. The antibacterial efficacy of nanosilver was checked by agar well diffusion method, and the silver nanoparticles showed effective antibacterial activity against Staphylococcus aureus.

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A Novel ‘Green’ Synthesis of Antimicrobial Silver Nanoparticles (AgNPs) by using Garcinia morella (Gaertn) Desr. Fruit Extract

Nanoscience &Nanotechnology-Asia ◽

10.2174/2210681205666150601215303 ◽

2015 ◽

Vol 5 (1) ◽

pp. 25-31 ◽

Cited By ~ 2

Author(s):

Debajit Borah ◽

Abhishek Kumar Yadav

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Fruit Extract

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Green synthesis of silver nanoparticles using Carica Papaya fruit extract under sunlight irradiation and their colorimetric detection of mercury ions

Journal of Physics Conference Series ◽

10.1088/1742-6596/817/1/012029 ◽

2017 ◽

Vol 817 ◽

pp. 012029 ◽

Cited By ~ 3

Author(s):

M Firdaus ◽

S Andriana ◽

Elvinawati ◽

W Alwi ◽

E Swistoro ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Carica Papaya ◽

Colorimetric Detection ◽

Mercury Ions ◽

Fruit Extract ◽

Sunlight Irradiation

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Green Synthesis of Gold and Silver Nanoparticles UsingAverrhoa bilimbiFruit Extract

Journal of Nanotechnology ◽

10.1155/2013/906592 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 39

Author(s):

R. S. Rimal Isaac ◽

G. Sakthivel ◽

Ch. Murthy

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Absorption Spectroscopy ◽

Metal Ion ◽

Cost Effective ◽

Average Diameter ◽

Ion Concentration ◽

Fruit Extract ◽

Gold And Silver Nanoparticles ◽

One Step

We report on rapid one-step green synthesis of gold and silver nanoparticles using fruit extract ofAverrhoa bilimbi Linn. UV-Vis absorption spectroscopy was used to monitor the quantitative formation of gold and silver nanoparticles. The characteristics of the obtained gold and silver nanoparticles were studied using UV-Vis absorption spectroscopy (UV/Vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and Energy-dispersive spectroscopy (EDX). UV/Vis spectrum showed Surface Plasmon Resonance (SPR) for both gold and silver nanoparticles at 540 and 420 nm. The EDX spectrum of the solution containing gold and silver nanoparticles confirmed the presence of elemental gold and silver signals. The average diameter of the prepared nanoparticles in solution was about 50–150 nm. Synthesized particles were either hexagonal or rhomboidal in shape. This synthesis approach of gold and silver nanoparticles is cost effective and can be widely used in biological systems. The effect of fruit extract and metal ion concentration was also studied.

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Green Synthesis of Silver Nanoparticles Using Diospyros malabarica Fruit Extract and Assessments of Their Antimicrobial, Anticancer and Catalytic Reduction of 4-Nitrophenol (4-NP)

Nanomaterials ◽

10.3390/nano11081999 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1999

Author(s):

Kaushik Kumar Bharadwaj ◽

Bijuli Rabha ◽

Siddhartha Pati ◽

Bhabesh Kumar Choudhury ◽

Tanmay Sarkar ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Environmental Protection Agency ◽

Catalytic Reduction ◽

The United States ◽

Single Step ◽

Diffusion Method ◽

Fruit Extract ◽

Primary Glioblastoma ◽

Pharmaceutical Industries

The green synthesis of silver nanoparticles (AgNPs) has currently been gaining wide applications in the medical field of nanomedicine. Green synthesis is one of the most effective procedures for the production of AgNPs. The Diospyros malabarica tree grown throughout India has been reported to have antioxidant and various therapeutic applications. In the context of this, we have investigated the fruit of Diospyros malabarica for the potential of forming AgNPs and analyzed its antibacterial and anticancer activity. We have developed a rapid, single-step, cost-effective and eco-friendly method for the synthesis of AgNPs using Diospyros malabarica aqueous fruit extract at room temperature. The AgNPs began to form just after the reaction was initiated. The formation and characterization of AgNPs were confirmed by UV-Vis spectrophotometry, XRD, FTIR, DLS, Zeta potential, FESEM, EDX, TEM and photoluminescence (PL) methods. The average size of AgNPs, in accordance with TEM results, was found to be 17.4 nm. The antibacterial activity of the silver nanoparticles against pathogenic microorganism strains of Staphylococcus aureus and Escherichia coli was confirmed by the well diffusion method and was found to inhibit the growth of the bacteria with an average zone of inhibition size of (8.4 ± 0.3 mm and 12.1 ± 0.5 mm) and (6.1 ± 0.7 mm and 13.1 ± 0.5 mm) at 500 and 1000 µg/mL concentrations of AgNPs, respectively. The anticancer effect of the AgNPs was confirmed by MTT assay using the U87-MG (human primary glioblastoma) cell line. The IC50 value was found to be 58.63 ± 5.74 μg/mL. The results showed that green synthesized AgNPs exhibited significant antimicrobial and anticancer potency. In addition, nitrophenols, which are regarded as priority pollutants by the United States Environmental Protection Agency (USEPA), can also be catalytically reduced to less toxic aminophenols by utilizing synthesized AgNPs. As a model reaction, AgNPs are employed as a catalyst in the reduction of 4-nitrophenol to 4-aminophenol, which is an intermediate for numerous analgesics and antipyretic drugs. Thus, the study is expected to help immensely in the pharmaceutical industries in developing antimicrobial drugs and/or as an anticancer drug, as well as in the cosmetic and food industries.

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