A reinforced nanofibrous patch with biomimetic mechanical properties and chondroinductive effect for rotator cuff tissue engineering

Hydrogels, as a class of materials for tissue engineering and drug delivery, have high water content and solid-like mechanical properties. Currently, hydrogels with an antibacterial function are a research hotspot in biomedical field. Many advanced antibacterial hydrogels have been developed, each possessing unique qualities, namely high water swellability, high oxygen permeability, improved biocompatibility, ease of loading and releasing drugs and structural diversity. In this article, an overview is provided on the preparation and applications of various antibacterial hydrogels. Furthermore, the prospects in biomedical researches and clinical applications are predicted.

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Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

International Journal of Molecular Sciences ◽

10.3390/ijms22073391 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3391

Author(s):

Sylwia Grabska-Zielińska ◽

Alina Sionkowska ◽

Ewa Olewnik-Kruszkowska ◽

Katarzyna Reczyńska ◽

Elżbieta Pamuła

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Physicochemical Properties ◽

Silk Fibroin ◽

Three Dimensional ◽

Microscopy Imaging ◽

Maximum Deformation ◽

One Step ◽

Chitosan Blends ◽

Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

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Development of Biopolymeric Hybrid Scaffold-Based on AAc/GO/nHAp/TiO2 Nanocomposite for Bone Tissue Engineering: In-Vitro Analysis

Nanomaterials ◽

10.3390/nano11051319 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1319

Author(s):

Muhammad Umar Aslam Khan ◽

Wafa Shamsan Al-Arjan ◽

Mona Saad Binkadem ◽

Hassan Mehboob ◽

Adnan Haider ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Guar Gum ◽

Porous Scaffolds ◽

Vitro Assay ◽

Vitro Analysis ◽

Nanocomposite Scaffolds

Bone tissue engineering is an advanced field for treatment of fractured bones to restore/regulate biological functions. Biopolymeric/bioceramic-based hybrid nanocomposite scaffolds are potential biomaterials for bone tissue because of biodegradable and biocompatible characteristics. We report synthesis of nanocomposite based on acrylic acid (AAc)/guar gum (GG), nano-hydroxyapatite (HAp NPs), titanium nanoparticles (TiO2 NPs), and optimum graphene oxide (GO) amount via free radical polymerization method. Porous scaffolds were fabricated through freeze-drying technique and coated with silver sulphadiazine. Different techniques were used to investigate functional group, crystal structural properties, morphology/elemental properties, porosity, and mechanical properties of fabricated scaffolds. Results show that increasing amount of TiO2 in combination with optimized GO has improved physicochemical and microstructural properties, mechanical properties (compressive strength (2.96 to 13.31 MPa) and Young’s modulus (39.56 to 300.81 MPa)), and porous properties (pore size (256.11 to 107.42 μm) and porosity (79.97 to 44.32%)). After 150 min, silver sulfadiazine release was found to be ~94.1%. In vitro assay of scaffolds also exhibited promising results against mouse pre-osteoblast (MC3T3-E1) cell lines. Hence, these fabricated scaffolds would be potential biomaterials for bone tissue engineering in biomedical engineering.

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Protein Hydrogels: The Swiss Army Knife for Enhanced Mechanical and Bioactive Properties of Biomaterials

Nanomaterials ◽

10.3390/nano11071656 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1656

Author(s):

Carla Huerta-López ◽

Jorge Alegre-Cebollada

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Polymer Networks ◽

Biological Tissues ◽

Modern Medicine ◽

Body Parts ◽

Biomedical Sciences ◽

Protein Hydrogels ◽

Large Water ◽

Design Options

Biomaterials are dynamic tools with many applications: from the primitive use of bone and wood in the replacement of lost limbs and body parts, to the refined involvement of smart and responsive biomaterials in modern medicine and biomedical sciences. Hydrogels constitute a subtype of biomaterials built from water-swollen polymer networks. Their large water content and soft mechanical properties are highly similar to most biological tissues, making them ideal for tissue engineering and biomedical applications. The mechanical properties of hydrogels and their modulation have attracted a lot of attention from the field of mechanobiology. Protein-based hydrogels are becoming increasingly attractive due to their endless design options and array of functionalities, as well as their responsiveness to stimuli. Furthermore, just like the extracellular matrix, they are inherently viscoelastic in part due to mechanical unfolding/refolding transitions of folded protein domains. This review summarizes different natural and engineered protein hydrogels focusing on different strategies followed to modulate their mechanical properties. Applications of mechanically tunable protein-based hydrogels in drug delivery, tissue engineering and mechanobiology are discussed.

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Rotator cuff anchor repair: Histological changes associated with the recovering mechanical properties in a rabbit model

Journal of Tissue Engineering and Regenerative Medicine ◽

10.1002/term.3195 ◽

2021 ◽

Author(s):

T. Mark Campbell ◽

Le Gao ◽

Odette Laneuville ◽

Hakim Louati ◽

Hans K. Uhthoff ◽

...

Keyword(s):

Mechanical Properties ◽

Rotator Cuff ◽

Rabbit Model ◽

Histological Changes

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Stress relaxation in tunable gels

Soft Matter ◽

10.1039/d1sm00091h ◽

2021 ◽

Author(s):

Chiara Raffaelli ◽

Wouter G Ellenbroek

Keyword(s):

Mechanical Properties ◽

Drug Delivery ◽

Tissue Engineering ◽

Stress Relaxation

Hydrogels are a staple of biomaterials development. Optimizing their use in e.g. drug delivery or tissue engineering requires a solid understanding of how to adjust their mechanical properties. Here, we...

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Role of Interfacial Interactions on Mechanical Properties of Biomimetic Composites for Bone Tissue Engineering

MRS Proceedings ◽

10.1557/proc-0898-l08-03 ◽

2005 ◽

Vol 898 ◽

Cited By ~ 1

Author(s):

Devendra Verma ◽

Rahul Bhowmik ◽

Bedabibhas Mohanty ◽

Dinesh R Katti ◽

Kalpana S Katti

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Mechanical Response ◽

Density Ratio ◽

Interfacial Interactions ◽

Porous Composites ◽

Properties Of Composites

AbstractInterfaces play an important role in controlling the mechanical properties of composites. Optimum mechanical strength of scaffolds is of prime importance for bone tissue engineering. In the present work, molecular dynamics simulations and experimental studies have been conducted to study effect of interfacial interactions on mechanical properties of composites for bone replacement. In order to mimic biological processes, hydroxyapatite (HAP) is mineralized in presence of polyacrylic acid (PAAc) (in situ HAP). Further, solid and porous composites of in situ HAP with polycaprolactone (PCL) are made. Mechanical tests of composites of in situ HAP with PAAc have shown improved strain recovery, higher modulus/density ratio and also improved mechanical response in simulated body fluid (SBF). Simulation studies indicate potential for calcium bridging between –COO− of PAAc and surface calcium of HAP. This fact is also supported by infrared spectroscopic studies. PAAc modified surfaces of in situ HAP offer means to control the microstructure and mechanical response of porous composites. Nanoindentation experiments indicate that apatite grown on in situ HAP/PCL composites from SBF has improved elastic modulus and hardness. This work gives insight into the interfacial mechanisms responsible for mechanical response as well as bioactivity in biomaterials.

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Hybrid nanostructured hydroxyapatite–chitosan composite scaffold: bioinspired fabrication, mechanical properties and biological properties

Journal of Materials Chemistry B ◽

10.1039/c5tb00175g ◽

2015 ◽

Vol 3 (23) ◽

pp. 4679-4689 ◽

Cited By ~ 50

Author(s):

Ya-Ping Guo ◽

Jun-Jie Guan ◽

Jun Yang ◽

Yang Wang ◽

Chang-Qing Zhang ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Composite Scaffold ◽

Biological Properties ◽

Chitosan Composite

A bioinspired strategy has been developed to fabricate a hybrid nanostructured hydroxyapatite–chitosan composite scaffold for bone tissue engineering.

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Additive manufacturing of PLA-based scaffolds intended for bone regeneration and strategies to improve their biological properties

e-Polymers ◽

10.1515/epoly-2020-0046 ◽

2020 ◽

Vol 20 (1) ◽

pp. 571-599

Author(s):

Ricardo Donate ◽

Mario Monzón ◽

María Elena Alemán-Domínguez

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Additive Manufacturing ◽

Bone Regeneration ◽

Polylactic Acid ◽

State Of The Art ◽

Biological Properties ◽

Design Stage ◽

Bone Scaffolds ◽

Regeneration Of Bone

AbstractPolylactic acid (PLA) is one of the most commonly used materials in the biomedical sector because of its processability, mechanical properties and biocompatibility. Among the different techniques that are feasible to process this biomaterial, additive manufacturing (AM) has gained attention recently, as it provides the possibility of tuning the design of the structures. This flexibility in the design stage allows the customization of the parts in order to optimize their use in the tissue engineering field. In the recent years, the application of PLA for the manufacture of bone scaffolds has been especially relevant, since numerous studies have proven the potential of this biomaterial for bone regeneration. This review contains a description of the specific requirements in the regeneration of bone and how the state of the art have tried to address them with different strategies to develop PLA-based scaffolds by AM techniques and with improved biofunctionality.

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